Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACP86_RS02415 Genome accession   NZ_CP011929
Coordinates   514726..515601 (+) Length   291 a.a.
NCBI ID   WP_053112654.1    Uniprot ID   A0A0K1U5R2
Organism   Marinobacter sp. CP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509726..520601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACP86_RS02400 (ACP86_02395) - 510833..511330 (-) 498 WP_053112651.1 pilin -
  ACP86_RS02405 (ACP86_02400) pilB 511718..513436 (+) 1719 WP_053112652.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACP86_RS02410 (ACP86_02405) pilC 513439..514659 (+) 1221 WP_053112653.1 type II secretion system F family protein Machinery gene
  ACP86_RS02415 (ACP86_02410) pilD 514726..515601 (+) 876 WP_053112654.1 prepilin peptidase Machinery gene
  ACP86_RS02420 (ACP86_02415) coaE 515605..516204 (+) 600 WP_053112655.1 dephospho-CoA kinase -
  ACP86_RS02425 (ACP86_02420) - 516197..517291 (+) 1095 WP_053112656.1 hypothetical protein -
  ACP86_RS02430 (ACP86_02425) tsaA 517291..518034 (+) 744 WP_053112657.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ACP86_RS02435 (ACP86_02430) - 518078..520429 (+) 2352 WP_053112658.1 response regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32224.33 Da        Isoelectric Point: 6.4640

>NTDB_id=149344 ACP86_RS02415 WP_053112654.1 514726..515601(+) (pilD) [Marinobacter sp. CP1]
MLSLETFLATPWLLFLTVTFVSLCIGSFLNVLILRLPKMMHQDWRCQCEEFLELPETQRKDEERITLSKPASTCPSCGHG
IRAWENIPVVSWLVLRGKCASCKAPISPRYPIIEALTAIFSVVTIAVIGPNESALWALLLVWALVALTVIDFDTQLLPDS
ITLPLMWLGLVLNFFGVLTDFNSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWQLLPAVILLSSV
VGAVVGISLMVFKKHGREVPIPFGPYLATAGLICLWFGSEIQAFWYGFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=149344 ACP86_RS02415 WP_053112654.1 514726..515601(+) (pilD) [Marinobacter sp. CP1]
ATGTTAAGTCTCGAAACCTTTCTTGCCACTCCGTGGCTCCTTTTTCTCACCGTTACCTTCGTGTCCCTCTGCATCGGCAG
TTTCCTGAATGTTTTGATTCTGCGACTGCCAAAAATGATGCATCAGGACTGGCGCTGCCAGTGCGAGGAATTCCTTGAGC
TGCCTGAAACACAGCGAAAAGATGAAGAGCGGATTACCCTGTCCAAACCGGCCTCCACCTGCCCTTCCTGCGGCCATGGC
ATTCGCGCCTGGGAGAACATCCCTGTAGTCAGCTGGCTGGTTCTTCGGGGCAAATGCGCATCCTGCAAGGCGCCTATCTC
GCCACGCTATCCAATCATTGAGGCGCTAACGGCAATTTTCTCGGTGGTGACCATTGCAGTCATCGGGCCGAACGAATCCG
CCCTCTGGGCCTTGTTGCTGGTGTGGGCTCTGGTGGCCCTGACCGTTATCGATTTCGACACCCAGCTCCTACCAGACAGC
ATTACGCTGCCCCTGATGTGGCTGGGCCTCGTGTTGAATTTTTTTGGCGTGCTGACGGACTTCAACAGTGCCTTTTGGGG
TGCGGTGGCAGGTTATCTCTCCTTGTGGTCTGTTTACTGGCTCTTCAAGCTGGTAACCGGCAAGGAGGGCATGGGCCACG
GAGACTTCAAACTGCTTGCCGCACTCGGCGCCTGGCTAGGGTGGCAGTTGTTGCCAGCGGTGATTCTGCTTTCCTCGGTT
GTAGGTGCCGTTGTGGGTATCAGCCTGATGGTGTTCAAGAAGCATGGGCGGGAAGTTCCGATTCCGTTTGGGCCATACCT
CGCGACAGCGGGGTTGATTTGTCTGTGGTTTGGCAGTGAGATTCAGGCATTCTGGTACGGATTCCTGGGAGTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1U5R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.839

94.158

0.526
  pilD Acinetobacter nosocomialis M2

55.109

94.158

0.519
  pilD Vibrio campbellii strain DS40M4

51.579

97.938

0.505
  pilD Vibrio cholerae strain A1552

52.5

96.22

0.505
  pilD Neisseria gonorrhoeae MS11

51.575

87.285

0.45

Multiple sequence alignment