Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB691_RS05105 Genome accession   NZ_CP011854
Coordinates   1089437..1091140 (+) Length   567 a.a.
NCBI ID   WP_048328642.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain SLG510A3-8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1084437..1096140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB691_RS05095 (AB691_1054) cysN 1085204..1087102 (+) 1899 WP_048328640.1 sulfate adenylyltransferase subunit CysN -
  AB691_RS21540 - 1087388..1088326 (-) 939 WP_230678778.1 O-antigen ligase family protein -
  AB691_RS22220 - 1088326..1088688 (+) 363 WP_230678780.1 hypothetical protein -
  AB691_RS05100 pilA2 1088690..1089115 (-) 426 WP_048328641.1 pilin Machinery gene
  AB691_RS05105 (AB691_1056) pilB 1089437..1091140 (+) 1704 WP_048328642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB691_RS05110 (AB691_1057) pilC 1091143..1092360 (+) 1218 WP_048328643.1 type II secretion system F family protein Machinery gene
  AB691_RS05115 (AB691_1058) pilD 1092365..1093231 (+) 867 WP_048328644.1 prepilin peptidase Machinery gene
  AB691_RS05120 (AB691_1059) coaE 1093363..1093971 (+) 609 WP_014595960.1 dephospho-CoA kinase -
  AB691_RS05125 yacG 1093968..1094165 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  AB691_RS05130 (AB691_1060) - 1094178..1094858 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  AB691_RS05135 (AB691_1061) - 1094915..1095955 (-) 1041 WP_048328645.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61953.96 Da        Isoelectric Point: 5.3366

>NTDB_id=149013 AB691_RS05105 WP_048328642.1 1089437..1091140(+) (pilB) [Stutzerimonas stutzeri strain SLG510A3-8]
MSDNISLSGLARQMVLAGVLDERAAQQAQVQALRNKSSLVTYVVQNKLAKGGVVAELASEQFGVALLDLAALDKEMLPKD
IVSEKLARQHRVLPLAKRGNKLYVAVSDPANHQAISDIQFSTGLTTEAILVEDDKLGDAIDKFFDNGSTGLEDLGDVDLD
GVDVETVSDDIKGDDGGAEADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVDVPRDVLLREGFP
EQKLGAFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPSLQRIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=149013 AB691_RS05105 WP_048328642.1 1089437..1091140(+) (pilB) [Stutzerimonas stutzeri strain SLG510A3-8]
ATGAGCGACAACATTTCACTTTCCGGCTTGGCCCGTCAGATGGTTCTGGCTGGTGTATTGGATGAGCGTGCTGCACAGCA
GGCGCAGGTGCAGGCGCTGCGAAACAAATCGTCCCTTGTTACTTACGTGGTCCAGAACAAGCTCGCAAAGGGGGGGGTCG
TTGCAGAGCTTGCATCCGAGCAGTTTGGCGTTGCGCTGCTGGATCTGGCGGCTTTGGACAAGGAGATGCTGCCTAAGGAT
ATTGTCAGCGAGAAGCTGGCTCGTCAGCATCGGGTGCTCCCGCTGGCGAAGCGTGGCAATAAGCTCTACGTCGCCGTTTC
CGACCCGGCTAACCATCAGGCGATTTCCGATATTCAGTTCAGTACTGGGTTAACGACCGAGGCCATCCTCGTCGAGGACG
ACAAGCTCGGGGACGCTATCGATAAATTTTTCGATAATGGTTCAACCGGTCTGGAGGATCTGGGCGACGTGGATCTCGAC
GGGGTGGATGTCGAGACGGTTTCGGATGACATAAAAGGAGACGATGGCGGTGCGGAGGCTGACGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATTCGTGGAGGCTCATCAGACCTCCATTTCGAGCCCTATGAAAAGGCCTATC
GAGTTCGGTTGCGTACTGACGGAATTCTGCATGAGGTGGCGCGTCCGCCCATTCAACTGGCGCCTCGAATTTCGGCGCGT
CTAAAGGTCATGGCAGGGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGAAGCTCTCCAAGAG
CAAGGCCATCGATTTCCGGGTCAACACGCTACCGACCCTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGACCCCTCAA
GCGCTCAAATGGGAATTGATGCGCTGGGTTATGAAGAGGATCAGAAAGAGCTGTACATGAATGCTCTGCGGCAACCGCAG
GGTATGATACTCGTCACCGGTCCGACGGGCTCCGGTAAGACAGTATCCCTCTACACGGGGTTGAACATCCTCAACACCGT
CGATGTGAACATTTCGACGGCGGAGGATCCGGTAGAGATCAACCTCGAGGGTATCAACCAAGTCAATGTCAATCCTAAGC
AGGGCATGGACTTTTCTCAGGCCCTCCGTGCGTTTCTACGTCAGGACCCGGACATCATCATGGTGGGGGAGATCCGCGAT
CTGGAGACCGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGTGATGTCGACACTTCATACCAATAGCGCTGC
GGAAACCCTGACGCGTCTGCGCAACATGGGGGTGCCTGCGTTCAACATCGCGACTTCCGTGAACCTGATCATTGCTCAGC
GTCTGGCACGAAAGCTCTGCAATAGCTGCAAGAAAGAGGTCGATGTCCCCCGTGACGTTTTGCTGCGCGAAGGGTTCCCT
GAGCAGAAGCTTGGAGCCTTTAAGATTTATGGTCCGGTAGGTTGTGACAACTGCAAGGGTGGCTACAAGGGGCGCGTCGG
TATTTATGAAGTGGTTAAAAACACGCCATCGTTGCAGCGGATTATCATGGAAGACGGAAACTCTATCGATATTTCAACCC
AGATGCGCAAAGACGGCTTCAACGACCTGCGGACCTCGGCTTTGCTGAAAGCCATGCAGGGCGTTACCAGCCTCGAAGAA
GTCAATCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.461

99.471

0.601
  pilB Acinetobacter baylyi ADP1

59.574

99.471

0.593
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio campbellii strain DS40M4

48.673

99.647

0.485
  pilB Vibrio cholerae strain A1552

53.216

90.476

0.481
  pilF Neisseria gonorrhoeae MS11

51.931

91.358

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

52.039

90.829

0.473
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

93.298

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.658

97.884

0.369

Multiple sequence alignment