Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACG10_RS04935 Genome accession   NZ_CP011835
Coordinates   1065542..1067242 (-) Length   566 a.a.
NCBI ID   WP_089167360.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain B3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1060542..1072242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG10_RS04905 (ACG10_04810) - 1060904..1061371 (+) 468 WP_089167355.1 FAD/FMN-containing dehydrogenase -
  ACG10_RS04910 (ACG10_04815) - 1061368..1062054 (+) 687 WP_089167356.1 energy-coupling factor ABC transporter permease -
  ACG10_RS04915 (ACG10_04820) yacG 1062075..1062275 (-) 201 WP_089167357.1 DNA gyrase inhibitor YacG -
  ACG10_RS04920 (ACG10_04825) coaE 1062272..1062880 (-) 609 WP_089167358.1 dephospho-CoA kinase -
  ACG10_RS04925 (ACG10_04830) pilD 1063029..1063898 (-) 870 WP_089167359.1 A24 family peptidase Machinery gene
  ACG10_RS04930 (ACG10_04835) pilC 1063901..1065118 (-) 1218 WP_089169794.1 type II secretion system F family protein Machinery gene
  ACG10_RS04935 (ACG10_04840) pilB 1065542..1067242 (-) 1701 WP_089167360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACG10_RS04940 (ACG10_04845) - 1067801..1068337 (+) 537 WP_089167361.1 pilin -
  ACG10_RS04945 (ACG10_04850) - 1068476..1069171 (+) 696 WP_157731375.1 hypothetical protein -
  ACG10_RS04950 (ACG10_04855) - 1069392..1069943 (+) 552 WP_089167363.1 REP-associated tyrosine transposase -
  ACG10_RS04955 (ACG10_04860) nadC 1070065..1070913 (-) 849 WP_089167364.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACG10_RS04960 (ACG10_04865) ampD 1071066..1071629 (+) 564 WP_089167365.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62473.55 Da        Isoelectric Point: 5.6466

>NTDB_id=148912 ACG10_RS04935 WP_089167360.1 1065542..1067242(-) (pilB) [Azotobacter chroococcum strain B3]
MTNDISLPGLARQMVLAGLIDEKTAQQAQQQAQRNQTPLVTWLVQNKLVKSRALAELAAEQFGIALFDLKTLDRESQPRD
LLSEKLIRQHRALPLWRRGNRLFVAISDPTNHEAIREIRFGTGLNTEAILVEDDRLGEAIEKYFEGAGTGLDDLADADLE
GLDVEASGQQDDEVNPAGDAEDAPVVRFINKMLLDAIRRGSSDLHFEPYEKSYRVRFRTDGILHEAARPPIQLAPKIAAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKTVMRILDPSSAQMGIDALGYEAEQKALYLAALSQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCACKKAVDIPREALLEEGFPE
ARIGTFKVYTPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEDGNSIEIARQMRADGFNDLRTSALLKAMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=148912 ACG10_RS04935 WP_089167360.1 1065542..1067242(-) (pilB) [Azotobacter chroococcum strain B3]
ATGACCAACGATATTTCCCTCCCCGGCCTGGCACGGCAGATGGTGCTGGCCGGGCTGATCGACGAGAAGACCGCCCAGCA
GGCCCAACAGCAGGCACAGCGCAACCAGACTCCCTTGGTCACCTGGCTGGTGCAGAACAAGCTGGTCAAGAGCCGGGCGC
TGGCAGAGCTGGCTGCCGAGCAGTTCGGCATCGCCCTGTTCGACCTCAAGACGCTGGACCGGGAGAGCCAGCCCCGGGAT
CTGCTCAGCGAGAAGCTGATCCGCCAGCACCGCGCCCTGCCGCTGTGGCGGCGCGGCAACCGGCTGTTCGTGGCGATTTC
CGACCCGACCAACCACGAGGCGATCCGCGAAATCCGCTTCGGCACCGGCTTGAACACCGAGGCCATCCTCGTCGAGGACG
ACCGGCTGGGCGAGGCCATCGAGAAATACTTCGAGGGCGCCGGCACCGGCCTGGACGACCTCGCCGATGCCGATCTGGAA
GGGCTCGACGTCGAGGCAAGCGGCCAGCAGGACGACGAAGTCAACCCGGCCGGCGATGCCGAGGATGCGCCGGTGGTGCG
TTTCATCAACAAGATGCTGCTGGATGCGATCCGCCGCGGCTCCTCGGACCTGCACTTCGAGCCCTACGAGAAGAGCTACC
GGGTGCGCTTCCGCACCGATGGCATCCTGCATGAGGCGGCCCGGCCGCCGATCCAGCTGGCGCCGAAGATCGCCGCGCGC
CTGAAGGTGATGGCCGGACTGGACATCTCCGAGCGGCGCAAGCCACAGGACGGCCGGATCAAGATGAAACTGTCGAAGAC
CAAGGCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGACGGTGATGCGGATTCTCGACCCGTCCA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGGCGGAACAGAAGGCGCTCTACCTGGCAGCGCTGAGCCAGCCGCAG
GGGATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCCCTGTATACCGGCCTGAACATCCTCAACACCCC
GGAGGTGAACATCTCCACCGCGGAGGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCACAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACTCTGACCCGCCTGCGCAACATGGGCGTGCCCTCGTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTGTGCGCCTGCAAGAAGGCGGTGGACATTCCCCGCGAGGCGCTGCTCGAGGAGGGCTTTCCGGAG
GCGCGCATCGGCACCTTCAAGGTCTACACCCCGGTCGGTTGCGAGAACTGCAACGGCGGCTACAAGGGCCGGGTCGGCAT
TTATGAAGTGGTTAAAATCACCCCGGCCCTGCAGCGCATTATCATGGAAGACGGCAACTCCATCGAAATTGCCCGGCAGA
TGCGCGCCGACGGCTTCAACGACCTGCGCACATCCGCCCTGCTGAAAGCCATGCAGGGTGTCACCAGCCTGGAAGAAGTC
AACCGCGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.702

99.647

0.615
  pilB Acinetobacter baylyi ADP1

58.596

100

0.59
  pilB Legionella pneumophila strain ERS1305867

54.386

100

0.548
  pilB Vibrio cholerae strain A1552

51.444

97.88

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.18

98.233

0.493
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Vibrio campbellii strain DS40M4

48.763

100

0.488
  pilB/pilB1 Synechocystis sp. PCC 6803

37.357

100

0.405
  pilF Thermus thermophilus HB27

37.943

99.647

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.544

92.933

0.367

Multiple sequence alignment