Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PGR6_RS24365 Genome accession   NZ_CP011567
Coordinates   5514676..5516376 (+) Length   566 a.a.
NCBI ID   WP_064620392.1    Uniprot ID   A0A2W7JGQ2
Organism   Pseudomonas sp. GR 6-02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5509676..5521376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGR6_RS24345 (PGR6_49110) - 5509890..5512886 (+) 2997 WP_177343105.1 DUF748 domain-containing protein -
  PGR6_RS24350 (PGR6_49120) - 5512886..5513182 (+) 297 WP_064620386.1 DUF2845 domain-containing protein -
  PGR6_RS24355 (PGR6_49130) - 5513356..5513712 (-) 357 WP_064620389.1 BON domain-containing protein -
  PGR6_RS24360 (PGR6_49140) - 5514039..5514449 (-) 411 WP_019650990.1 pilin -
  PGR6_RS24365 (PGR6_49150) pilB 5514676..5516376 (+) 1701 WP_064620392.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PGR6_RS24370 (PGR6_49160) pilC 5516379..5517596 (+) 1218 WP_018930188.1 type II secretion system F family protein Machinery gene
  PGR6_RS24375 (PGR6_49170) pilD 5517598..5518470 (+) 873 WP_064620394.1 A24 family peptidase Machinery gene
  PGR6_RS24380 (PGR6_49180) coaE 5518467..5519090 (+) 624 WP_026286732.1 dephospho-CoA kinase -
  PGR6_RS24385 (PGR6_49190) yacG 5519087..5519287 (+) 201 WP_007938961.1 DNA gyrase inhibitor YacG -
  PGR6_RS24390 (PGR6_49200) - 5519297..5519512 (-) 216 WP_007938959.1 hypothetical protein -
  PGR6_RS24395 (PGR6_49210) - 5519576..5520265 (-) 690 WP_018930184.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62956.98 Da        Isoelectric Point: 5.6938

>NTDB_id=146941 PGR6_RS24365 WP_064620392.1 5514676..5516376(+) (pilB) [Pseudomonas sp. GR 6-02]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRDRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLEKDTQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPSNQQVINDIQFSTGLNTEAILVEEDKLSDAIERFFDSHATDLQEMADVDLDG
LDIESVDDQKQDAIGGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILHEVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINMEGVNQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCNQCNAGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=146941 PGR6_RS24365 WP_064620392.1 5514676..5516376(+) (pilB) [Pseudomonas sp. GR 6-02]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
GTATCAGCAAGCCCAACGCGATCGAGTCTCACTGGTCAGTTATCTGGTGCAAAACAAATTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGAAAAAGACACCCAACCCAAGGGGCTG
GTCAGCGAAAAACTGATCCGCCAGCACCACGCCCTACCCCTCTGGCGACGCGGTAACAAACTGTTCGTGGGGGTTTCCGA
TCCGAGTAATCAACAGGTCATCAACGACATCCAATTCAGTACCGGGCTGAACACCGAAGCCATCCTGGTCGAAGAGGACA
AGCTTAGCGACGCCATCGAAAGATTCTTCGACAGCCACGCCACCGACCTGCAAGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGTCGACGACCAGAAACAGGACGCCATCGGCGGACAGGATACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGGTCTTCCGACCTGCATTTCGAACCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAAGTTGCCAAGCCACCAACGCAACTGGCCAACCGCATTGCTTCCCGGCTC
AAAGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGAATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTTGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTCGGCTATGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCACAGGGG
ATGATCCTGGTGACCGGGCCCACCGGTTCAGGCAAGACCGTGTCGCTCTATACCGGACTGAACATCCTCAACACCGTCAA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAGGGCGTCAACCAGGTCAACGTCAATCCCAGGCAGG
GAATGGACTTCGCCCAGGCGTTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACTGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCTGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGCTTCAACATTGCGACCTCGGTCAGCCTGATCATTGCCCAGCGCC
TGGCGCGCAAGTTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCATTCACGATCTATGAACCGGTCGGTTGCAATCAATGCAATGCTGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACTCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAGATCGACCTCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACGTCGGGCTTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7JGQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.876

99.823

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.617

100

0.507
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

49.64

98.233

0.488
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilF Thermus thermophilus HB27

36.572

100

0.366

Multiple sequence alignment