Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABA45_RS04595 Genome accession   NZ_CP011494
Coordinates   1014868..1016583 (-) Length   571 a.a.
NCBI ID   WP_048384502.1    Uniprot ID   A0A0H4HYK1
Organism   Marinobacter psychrophilus strain 20041     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1009868..1021583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABA45_RS04565 (ABA45_04565) yacG 1009985..1010173 (-) 189 WP_048384497.1 DNA gyrase inhibitor YacG -
  ABA45_RS04570 (ABA45_04570) tsaA 1010247..1011014 (-) 768 WP_048384498.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ABA45_RS04575 (ABA45_04575) - 1011020..1012129 (-) 1110 WP_198147056.1 hypothetical protein -
  ABA45_RS04580 (ABA45_04580) coaE 1012101..1012709 (-) 609 WP_048384499.1 dephospho-CoA kinase -
  ABA45_RS04585 (ABA45_04585) pilD 1012766..1013641 (-) 876 WP_048384500.1 A24 family peptidase Machinery gene
  ABA45_RS04590 (ABA45_04590) pilC 1013645..1014865 (-) 1221 WP_048384501.1 type II secretion system F family protein Machinery gene
  ABA45_RS04595 (ABA45_04595) pilB 1014868..1016583 (-) 1716 WP_048384502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABA45_RS04600 (ABA45_04600) - 1017159..1017602 (-) 444 WP_048384503.1 pilin -
  ABA45_RS04605 (ABA45_04605) - 1017671..1019461 (-) 1791 WP_048384504.1 PglL family O-oligosaccharyltransferase -
  ABA45_RS04610 (ABA45_04610) - 1019536..1020000 (-) 465 WP_048384505.1 pilin -
  ABA45_RS19230 (ABA45_04615) - 1020571..1020941 (+) 371 Protein_910 antibiotic biosynthesis monooxygenase family protein -
  ABA45_RS04620 (ABA45_04620) - 1020910..1021500 (+) 591 WP_227506127.1 DUF2784 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62407.55 Da        Isoelectric Point: 5.4695

>NTDB_id=146338 ABA45_RS04595 WP_048384502.1 1014868..1016583(-) (pilB) [Marinobacter psychrophilus strain 20041]
MTTNTSNITLTGLARRFVSDGLLDESTAKDAFFQASQNRIPLITYLTQHKLANSSALAFSAAMEFGISVLDLDAFLPEAM
PDKLVDEKLLRKHNALPLYKRGNRLFIAVSDPTNVQALDEIKFNTGLSTDAILVDDSKLRAAIEKYMASSESSMGDLADT
DLEGIETEGADGNEDDGAVVVAEIDDAPIVKYVNKMLLDAIRGGSSDVHFEPYEKTYRVRFRTDGILKEMSRPSIKLAPK
ISARIKIMAQLDISERRLPQDGRIKMRLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTDERNISTAEDPAEINLEGVNQVNVNNKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQAGSIPKDVLLAE
GFSTEQIATGFTLYHPKGCDKCTNGYKGRVGIYEVVKVTEQLANMIMEEASSIKIAKAAQAEGFRTLRQSALLKVIEGLT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=146338 ABA45_RS04595 WP_048384502.1 1014868..1016583(-) (pilB) [Marinobacter psychrophilus strain 20041]
ATGACAACAAACACAAGCAACATCACCTTAACGGGTCTGGCACGGCGCTTTGTAAGCGACGGCCTGCTGGATGAATCCAC
GGCCAAAGACGCCTTTTTTCAGGCTTCTCAGAACCGCATTCCGCTGATCACCTACCTGACACAGCACAAGTTGGCAAACA
GCTCGGCGTTGGCGTTTTCGGCGGCTATGGAGTTTGGTATTTCAGTGCTGGACCTGGATGCGTTTTTGCCAGAGGCGATG
CCAGACAAGCTGGTGGATGAAAAACTGCTGCGCAAGCACAACGCCCTGCCCCTGTATAAGCGTGGCAACCGGCTGTTTAT
TGCGGTGAGCGACCCCACCAACGTTCAGGCTCTGGACGAGATCAAGTTCAACACCGGCTTAAGTACCGATGCGATTCTGG
TGGACGACTCCAAACTGCGCGCAGCTATCGAAAAGTATATGGCGTCCAGTGAAAGCTCCATGGGCGACTTGGCCGATACA
GACCTTGAAGGTATAGAAACAGAAGGCGCGGATGGAAACGAGGATGACGGCGCTGTTGTTGTTGCAGAGATAGACGATGC
GCCCATCGTGAAGTATGTGAATAAGATGCTGCTGGATGCCATTCGCGGAGGCTCGTCAGACGTTCACTTTGAACCCTACG
AGAAAACTTACCGCGTACGCTTTCGCACCGACGGCATTTTGAAAGAAATGTCGCGACCCTCTATAAAGCTGGCACCGAAA
ATATCTGCGCGAATAAAGATCATGGCACAGTTGGACATTTCCGAGCGACGTTTGCCACAAGACGGCCGCATAAAGATGAG
GCTATCCAAAACCAAGGCCATCGACTTTCGGGTGAACACACTGCCCACGCTGTGGGGGGAGAAAATCGTACTGCGAATTC
TGGACCCCAGCCAAGCCAAGCTGGGCATTGATGCGCTTGGGTATGAAGAAGATCAGAAGAAGCTGTATATGGACGCCTTG
GCGCAGCCCCAAGGTATGATTCTGGTGACGGGCCCCACCGGTTCCGGTAAAACCGTATCGTTGTATACTGGCTTGAATAT
TCTAAACACTGACGAGCGCAATATTTCCACGGCGGAAGACCCGGCGGAAATTAACCTGGAAGGCGTAAATCAGGTCAACG
TGAACAACAAGGTAGGGCTTGGCTTTGCCGAGGCGCTAAGAGCGTTTTTGCGGCAAGACCCTGACGTTATCATGGTGGGC
GAGATTCGAGACCTGGAAACCGCTAACATTGCCATCAAAGCGGCACAAACCGGTCACCTTGTGTTGTCCACGTTGCACAC
CAACAGCGCCGCCGAAACCTTAACCCGCATGATGAACATGGGCGTGCCGGCGTTTAACATTGCCACGTCTGTTAGCCTGA
TTATTGCCCAGCGCCTGGGCCGGCGACTTTGCAGCTGCAAACAGGCTGGAAGCATCCCGAAAGACGTCCTTTTAGCCGAA
GGGTTCTCAACTGAACAAATTGCAACCGGGTTCACGTTGTACCACCCGAAGGGCTGTGATAAATGCACTAATGGGTATAA
AGGCCGCGTCGGCATTTACGAGGTGGTTAAAGTGACCGAGCAGCTGGCCAACATGATTATGGAAGAAGCCAGTTCTATCA
AGATTGCAAAAGCAGCTCAGGCTGAAGGCTTTCGGACCCTGCGCCAATCCGCCCTGTTAAAGGTGATTGAGGGTTTGACC
AGCCTTGAAGAAGCCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H4HYK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.923

100

0.562
  pilB Acinetobacter baylyi ADP1

54.625

100

0.548
  pilB Legionella pneumophila strain ERS1305867

53.206

100

0.538
  pilB Vibrio campbellii strain DS40M4

50.973

98.949

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

96.848

0.501
  pilB Vibrio cholerae strain A1552

53.95

90.893

0.49
  pilF Neisseria gonorrhoeae MS11

47.08

98.949

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

41.779

90.543

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

92.995

0.363

Multiple sequence alignment