Detailed information NTG0146171

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IMCC21906_RS09405
• Genome accession: NZ_CP011477
• Coordinates: 2044607..2045773 (-)
• Length: 388 a.a.
• NCBI ID: WP_047011954.1
• Uniprot ID: A0A0F7M2A7
• Organism: Spongiibacter sp. IMCC21906
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2039607..2050773

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IMCC21906_RS09390 (IMCC21906_01903)	fdxA	2040114..2040437 (-)	324	WP_047011951.1	ferredoxin FdxA	-
IMCC21906_RS09395 (IMCC21906_01904)	mutS	2040579..2043149 (-)	2571	WP_047011952.1	DNA mismatch repair protein MutS	-
IMCC21906_RS09400 (IMCC21906_01905)	recA	2043355..2044383 (+)	1029	WP_047011953.1	recombinase RecA	Machinery gene
IMCC21906_RS09405 (IMCC21906_01906)	pilU	2044607..2045773 (-)	1167	WP_047011954.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IMCC21906_RS09415 (IMCC21906_01908)	-	2046430..2048505 (-)	2076	WP_082117431.1	pilus assembly protein TadG-related protein	-
IMCC21906_RS09420 (IMCC21906_01909)	-	2048517..2048771 (-)	255	WP_047011957.1	DUF3613 domain-containing protein	-
IMCC21906_RS09425 (IMCC21906_01910)	-	2048768..2049469 (-)	702	WP_047011958.1	hypothetical protein	-
IMCC21906_RS09430 (IMCC21906_01911)	-	2049515..2050390 (-)	876	WP_047011959.1	type II secretion system F family protein	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42990.17 Da
• Isoelectric Point: 6.2396

>NTDB_id=146171 IMCC21906_RS09405 WP_047011954.1 2044607..2045773(-) (pilU) [Spongiibacter sp. IMCC21906]
MTFNDYLTILAKNAGSDLFLSTGAPPCAKFHGQLKPLNKEPFSSGDIKKIAYEIMDSEQIKEFELELEMNLAYSIAKVGR
FRVNIFKQRNEISIVARNIVTDIPKVDDLGLPPILKDVVMTKRGLVLFVGATGSGKSTSLAALIDHRNTSSSGHIITIED
PIEFIHRHKKSIINQREIGVDTRSFHQALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCISTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKCFVSQRLVPTVDGKRAAAIEVLLGTPTVAQAIHKGEIEGIKEIMAKSENLGMQTFDAAL
FKLYEDGKISLEDALKNADSANNLRLRIKLHSSRGLPVGAHQEAESEATNTGFSLTIEEPDDGDEQIF

Nucleotide

• Length: 1167 bp

>NTDB_id=146171 IMCC21906_RS09405 WP_047011954.1 2044607..2045773(-) (pilU) [Spongiibacter sp. IMCC21906]
ATGACCTTCAACGACTACCTTACCATCCTTGCCAAAAACGCTGGCTCCGACCTGTTCCTCAGCACCGGCGCCCCACCCTG
CGCCAAGTTTCACGGCCAGCTTAAGCCGCTGAATAAAGAGCCTTTTTCTAGCGGCGATATTAAAAAGATTGCTTATGAAA
TTATGGATAGTGAGCAGATTAAGGAGTTTGAGCTAGAGCTGGAAATGAACCTAGCCTATAGCATCGCCAAGGTCGGACGC
TTCCGGGTCAACATTTTCAAACAACGCAATGAAATTTCTATTGTGGCTCGTAATATTGTTACTGACATCCCCAAAGTTGA
TGATTTGGGCTTACCGCCAATACTTAAAGATGTGGTGATGACAAAGCGTGGCTTAGTACTTTTTGTTGGCGCTACCGGAT
CGGGTAAATCTACTTCTCTTGCTGCTCTGATCGATCATCGCAATACCAGCAGTAGCGGTCATATTATTACTATTGAAGAT
CCCATTGAATTTATTCACCGCCACAAGAAAAGCATTATCAACCAGCGTGAGATTGGCGTTGATACAAGGAGTTTTCACCA
AGCATTGAAAAACACGTTGAGGCAAGCACCGGACGTCATTTTGATCGGTGAAATACGCGATAGAGAGACAATGGAGCATG
CAATCGCCTTTGCCGAGACGGGTCACCTTTGTATCTCAACATTGCACGCTAACAATGCTAACCAAGCCTTGGATAGAATT
ATTAACTTCTTTCCGGAAGAGCGCAGAAATCAATTATTATTAGACTTGTCACTGAATTTAAAATGCTTTGTTTCTCAGCG
TTTAGTACCCACTGTAGATGGGAAACGCGCTGCCGCGATTGAAGTTTTACTAGGTACCCCGACAGTCGCTCAAGCGATTC
ATAAAGGAGAAATCGAAGGCATTAAAGAAATTATGGCCAAGTCTGAAAACTTAGGCATGCAAACCTTTGACGCCGCCTTA
TTCAAACTTTATGAAGACGGGAAAATCAGTCTTGAAGACGCATTAAAAAATGCCGACTCTGCTAACAATCTCCGCCTTAG
AATCAAGCTGCACAGCAGTAGAGGCTTGCCCGTCGGCGCCCACCAAGAAGCTGAATCCGAAGCAACAAATACAGGGTTTA
GCTTAACAATAGAAGAACCGGACGATGGCGACGAACAAATTTTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-270)

3D structure

• AlphaFold DB: A0A0F7M2A7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.18	98.711	0.564
pilU	Acinetobacter baylyi ADP1	56.667	92.784	0.526
pilU	Vibrio cholerae strain A1552	50.97	93.041	0.474
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.732	98.196	0.41
pilT	Legionella pneumophila strain Lp02	43.03	85.052	0.366
pilT	Legionella pneumophila strain ERS1305867	43.03	85.052	0.366