Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IMCC21906_RS06045 Genome accession   NZ_CP011477
Coordinates   1319756..1321474 (-) Length   572 a.a.
NCBI ID   WP_047011417.1    Uniprot ID   -
Organism   Spongiibacter sp. IMCC21906     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1314756..1326474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMCC21906_RS06020 (IMCC21906_01227) mutT 1315420..1315833 (+) 414 WP_047011412.1 8-oxo-dGTP diphosphatase MutT -
  IMCC21906_RS06025 (IMCC21906_01228) - 1316749..1316964 (-) 216 WP_047011413.1 DNA gyrase inhibitor YacG -
  IMCC21906_RS06030 (IMCC21906_01229) coaE 1316961..1317566 (-) 606 WP_047011414.1 dephospho-CoA kinase -
  IMCC21906_RS06035 (IMCC21906_01230) pilD 1317614..1318483 (-) 870 WP_047011415.1 A24 family peptidase Machinery gene
  IMCC21906_RS06040 (IMCC21906_01231) pilC 1318538..1319752 (-) 1215 WP_047011416.1 type II secretion system F family protein Machinery gene
  IMCC21906_RS06045 (IMCC21906_01232) pilB 1319756..1321474 (-) 1719 WP_047011417.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IMCC21906_RS06050 (IMCC21906_01233) - 1322189..1322662 (+) 474 WP_047011418.1 pilin -
  IMCC21906_RS06055 (IMCC21906_01234) - 1322666..1324135 (+) 1470 WP_082117378.1 O-antigen ligase -
  IMCC21906_RS06060 (IMCC21906_01235) nadC 1324203..1325081 (-) 879 WP_047011420.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62721.83 Da        Isoelectric Point: 5.0315

>NTDB_id=146164 IMCC21906_RS06045 WP_047011417.1 1319756..1321474(-) (pilB) [Spongiibacter sp. IMCC21906]
MNTALPPLNGLARRLVYDGVLEEQDVRKAMEAAQKEKKTLVSYLVSKNLADGLQIAEAAADEFGVPFLDLRAYLPELAPQ
KLVDSKLVRSQHALPLWKRGKRLFLGMSDPTNLGALDQIKFHTGLNTEPVVVEESALHAAIDKYLASQEESLSDSFGDMD
DIGLENLDIEAVDEDNNAEVDLKGADEAPVVKFINKVLVDAIRGGSSDVHFEPYEKIYRVRFRTDGILHEVARPPAALGY
RLAARLKVMSQMDISERRVPQDGRIKMKLSKTRSIDFRVNTLPTLWGEKVVLRILDPSSAQMGIDALGYEEEQKALYMKC
LNQPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVNAKVGLGFAEALRSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSFNVATSVSLIIAQRLARRLCKDCAAPADIPEQTLL
EEGFTLEQIESASILAPVGCDKCNNGYKGRVGIYEVVRITPEISHIIMENGNSLEIAAKAAEQGFANLRKSALKKCAQGV
TSLEEVNRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=146164 IMCC21906_RS06045 WP_047011417.1 1319756..1321474(-) (pilB) [Spongiibacter sp. IMCC21906]
ATGAATACAGCACTCCCACCGCTAAACGGTCTTGCCAGACGCCTGGTCTACGATGGCGTTCTTGAAGAACAGGATGTTCG
TAAAGCCATGGAGGCTGCCCAAAAAGAAAAGAAAACGCTGGTGTCTTATCTTGTCAGCAAAAACCTGGCAGACGGGCTGC
AGATTGCCGAGGCCGCGGCGGATGAGTTTGGGGTGCCGTTTTTAGACCTCAGGGCTTACCTCCCAGAACTGGCACCGCAA
AAGTTGGTAGATAGCAAGCTGGTACGCAGCCAGCACGCCTTACCGTTATGGAAACGGGGCAAGCGCTTGTTTCTGGGCAT
GTCTGACCCCACCAATCTTGGTGCCCTTGACCAAATCAAGTTTCACACCGGTCTGAATACCGAGCCGGTGGTGGTTGAAG
AATCGGCTCTGCACGCAGCGATAGATAAGTACCTTGCCTCCCAAGAAGAGTCTTTGTCTGACAGCTTTGGCGACATGGAC
GATATCGGCCTTGAAAACCTCGATATAGAAGCGGTCGACGAAGATAACAATGCCGAAGTGGATCTTAAGGGTGCCGACGA
GGCGCCGGTCGTTAAATTTATTAATAAAGTGTTGGTGGATGCCATACGCGGCGGCTCATCGGATGTGCACTTTGAACCCT
ATGAAAAAATCTATCGGGTACGGTTTCGAACTGACGGCATTCTTCATGAAGTGGCCCGCCCGCCCGCAGCATTAGGCTAT
CGTTTGGCAGCGCGTTTAAAAGTCATGTCGCAAATGGATATCTCCGAGCGCCGCGTTCCCCAAGATGGCCGCATTAAGAT
GAAGCTATCCAAAACCCGCTCCATCGATTTTCGGGTCAACACCCTGCCTACGTTGTGGGGCGAAAAAGTGGTGCTACGGA
TTTTGGACCCCAGCTCGGCGCAAATGGGTATTGACGCACTGGGCTATGAGGAAGAACAAAAAGCCCTGTATATGAAGTGC
CTGAATCAACCCCAAGGGATGATATTGGTCACTGGCCCTACCGGCTCGGGTAAAACCGTCTCGCTATACACCGGGCTGAA
TATTCTAAATACTGAGGAGCGCAACATCTCCACCGCCGAAGACCCCGTGGAGATCAACCTGGAAGGGATTAACCAGGTTC
ACGTCAACGCAAAAGTCGGTCTAGGCTTTGCAGAAGCGCTGCGCTCATTCCTGCGTCAAGACCCCGATATTATCATGGTG
GGGGAAATACGCGATTTAGAGACGGCAGAGATTGCCATTAAAGCGGCGCAAACCGGTCATTTAGTGCTATCGACCCTGCA
CACCAATAGCGCTGCCGAAACCCTGACCCGTTTGCTGAATATGGGCGTGCCTTCTTTTAACGTGGCAACGTCGGTGAGCC
TGATTATTGCTCAACGCCTGGCAAGACGCCTGTGTAAAGACTGCGCGGCCCCTGCCGACATCCCAGAACAAACCTTGCTG
GAAGAAGGCTTTACCCTAGAACAAATTGAGTCTGCCAGCATTCTTGCCCCAGTGGGTTGTGATAAGTGTAATAACGGCTA
CAAAGGCCGGGTGGGCATTTACGAGGTTGTGCGTATTACCCCTGAGATTTCGCATATTATTATGGAGAATGGTAACTCTC
TGGAAATTGCCGCTAAAGCGGCCGAACAGGGCTTTGCCAATTTACGTAAATCTGCGCTTAAAAAATGTGCTCAAGGGGTT
ACCAGCCTCGAAGAAGTCAACCGGGTAACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.522

99.825

0.624
  pilB Acinetobacter baylyi ADP1

61.34

100

0.624
  pilB Legionella pneumophila strain ERS1305867

55.203

99.126

0.547
  pilB Vibrio parahaemolyticus RIMD 2210633

53.208

92.657

0.493
  pilB Vibrio campbellii strain DS40M4

49.645

98.601

0.49
  pilB Vibrio cholerae strain A1552

53.488

90.21

0.483
  pilF Neisseria gonorrhoeae MS11

47.972

99.126

0.476
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.94

100

0.378
  pilF Thermus thermophilus HB27

36.713

100

0.367

Multiple sequence alignment