Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TVD_RS02265 Genome accession   NZ_CP011367
Coordinates   481461..483179 (-) Length   572 a.a.
NCBI ID   WP_047250714.1    Uniprot ID   -
Organism   Thioalkalivibrio versutus strain D301     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476461..488179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TVD_RS02240 (TVD_02240) - 476848..477822 (+) 975 WP_047250712.1 Nudix family hydrolase -
  TVD_RS02245 (TVD_02245) zapD 477855..478625 (-) 771 WP_018939032.1 cell division protein ZapD -
  TVD_RS02250 (TVD_02250) coaE 478685..479329 (-) 645 WP_019563203.1 dephospho-CoA kinase -
  TVD_RS02255 (TVD_02255) pilD 479339..480202 (-) 864 WP_047250713.1 prepilin peptidase Machinery gene
  TVD_RS02260 (TVD_02260) pilC 480206..481417 (-) 1212 WP_018144450.1 type II secretion system F family protein Machinery gene
  TVD_RS02265 (TVD_02265) pilB 481461..483179 (-) 1719 WP_047250714.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TVD_RS02285 (TVD_02285) tuf 483800..484990 (+) 1191 WP_018168213.1 elongation factor Tu -
  TVD_RS02295 (TVD_02295) secE 485190..485555 (+) 366 WP_018167854.1 preprotein translocase subunit SecE -
  TVD_RS02300 (TVD_02300) nusG 485573..486106 (+) 534 WP_017940468.1 transcription termination/antitermination protein NusG -
  TVD_RS02305 (TVD_02305) rplK 486172..486603 (+) 432 WP_018650512.1 50S ribosomal protein L11 -
  TVD_RS02310 (TVD_02310) rplA 486605..487300 (+) 696 WP_047250715.1 50S ribosomal protein L1 -
  TVD_RS02315 (TVD_02315) rplJ 487515..488036 (+) 522 WP_018143869.1 50S ribosomal protein L10 -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62334.23 Da        Isoelectric Point: 4.9631

>NTDB_id=145154 TVD_RS02265 WP_047250714.1 481461..483179(-) (pilB) [Thioalkalivibrio versutus strain D301]
MANTTTAPRLPGLAKRLVEAGLIAEDIASQLVEEAQSQQTPLMTLLASKGQVAEARLVEAAAEEFGLPVFDLGSFDAELL
PKDHLNEKLIQKHHALPLMARGNRLYIGVSDPTNLGATDEFKFATGMPVEAVLVEPHKLDRLIDTALSAASSLMDDLGDD
GLDGLAIESGGEDDKESSDTAEGADDAPVVRFVNKVLMDAINRKASDIHLEPFEKDFRVRFRIDGVLHEVAHPPNSLAPR
ITARVKVMSRMDIAERRVPQDGRIKLKLSRTRAIDFRVSSLPTLYGEKIVMRILDPSSAAMGIDQLGYEEHQKEAYLKAL
SRPYGMILVTGPTGSGKTVSLYTGLGILNTADRNISTAEDPSEINMPGVNQVNINPKVGLTFASALRAFLRQDPDVIMVG
EIRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPYNIASSILLIIAQRLARRLCPNCKEPEKLPEEALRK
EGFTDADIQNGVVSYRAVGCDQCTNGYKGRLGIYQVMPISDDMQRGILEGYNSMQLAEQAEKEGIEDLRRAGLVKVMAGL
TSLEEVNRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=145154 TVD_RS02265 WP_047250714.1 481461..483179(-) (pilB) [Thioalkalivibrio versutus strain D301]
ATGGCGAATACCACTACGGCACCGCGGCTACCCGGGCTGGCGAAGCGCCTGGTCGAGGCCGGCCTGATCGCGGAGGACAT
CGCCTCCCAGCTGGTCGAGGAAGCCCAGTCCCAGCAGACGCCGCTGATGACCCTGCTTGCCAGCAAGGGCCAGGTGGCGG
AAGCCCGCCTGGTCGAGGCCGCCGCCGAGGAATTCGGGCTCCCGGTATTCGACCTGGGGAGCTTCGATGCGGAACTGCTG
CCCAAGGATCACCTCAACGAGAAGCTGATCCAGAAGCACCATGCCCTGCCACTGATGGCACGCGGCAATCGCCTCTACAT
CGGCGTGTCCGACCCGACCAACCTCGGTGCCACCGACGAGTTCAAGTTCGCCACCGGCATGCCCGTCGAGGCCGTCCTGG
TCGAGCCACACAAGCTCGACCGCCTGATCGACACGGCACTGAGTGCCGCCTCGTCGCTGATGGACGACCTTGGCGACGAC
GGCCTCGACGGGCTGGCCATCGAATCCGGTGGGGAGGATGACAAGGAGTCCTCCGACACCGCCGAGGGCGCGGATGACGC
CCCGGTGGTGCGCTTCGTCAACAAGGTTCTGATGGACGCCATCAATCGCAAGGCGTCGGATATCCACCTCGAGCCCTTCG
AGAAGGACTTCCGCGTGCGCTTCCGCATTGACGGCGTACTGCACGAGGTCGCCCACCCACCCAATTCCCTGGCGCCGCGC
ATCACTGCGCGCGTCAAGGTGATGTCGCGCATGGACATCGCCGAACGCCGCGTGCCGCAGGACGGGCGCATCAAGCTTAA
ACTGTCGCGTACACGCGCGATCGATTTTCGTGTCTCGTCCCTGCCGACGCTTTACGGCGAGAAGATCGTGATGCGTATCC
TCGATCCCAGCAGCGCGGCGATGGGCATCGATCAGCTGGGGTACGAGGAACATCAGAAAGAGGCCTACCTCAAGGCGCTC
AGCCGCCCGTACGGCATGATCCTGGTCACCGGCCCCACCGGGTCGGGGAAGACCGTGTCGCTGTACACCGGGCTCGGCAT
CCTGAACACCGCCGACCGCAACATCTCCACTGCGGAAGACCCGTCGGAAATCAACATGCCCGGGGTCAACCAGGTCAACA
TCAACCCGAAGGTGGGTCTGACCTTTGCCAGCGCGCTGCGCGCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGG
GAAATCCGCGACCTGGAAACCGCCGAGATCTCCATCAAGGCGGCCCAGACCGGTCACCTTGTGCTTTCCACGCTGCATAC
CAACGACGCCCCGCAGACGCTCACCCGCCTGGCCAACATGGGCGTGCCACCGTACAACATCGCCTCCTCGATCCTGCTGA
TCATTGCCCAGCGGCTGGCGCGGCGGTTGTGCCCGAACTGCAAGGAACCGGAAAAGCTCCCGGAAGAGGCGCTGCGCAAG
GAAGGCTTTACCGACGCCGACATCCAGAACGGTGTGGTCTCCTATCGCGCGGTGGGCTGCGACCAATGCACCAACGGCTA
CAAGGGCCGGCTGGGGATCTACCAGGTCATGCCCATCAGCGACGACATGCAGCGCGGCATCCTGGAGGGCTACAACTCCA
TGCAGCTCGCAGAACAAGCAGAAAAGGAAGGCATCGAGGACCTGCGACGCGCCGGCCTCGTGAAAGTAATGGCGGGGCTG
ACCAGCCTCGAGGAAGTCAATCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.927

100

0.54
  pilB Acinetobacter baumannii D1279779

53.752

100

0.538
  pilB Legionella pneumophila strain ERS1305867

52.332

100

0.53
  pilF Neisseria gonorrhoeae MS11

50

98.601

0.493
  pilB Vibrio campbellii strain DS40M4

48.582

98.601

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

48.663

98.077

0.477
  pilB Vibrio cholerae strain A1552

52.51

90.559

0.476
  pilF Thermus thermophilus HB27

38.811

100

0.388

Multiple sequence alignment