Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TVD_RS02255 Genome accession   NZ_CP011367
Coordinates   479339..480202 (-) Length   287 a.a.
NCBI ID   WP_047250713.1    Uniprot ID   A0A0G3FZB6
Organism   Thioalkalivibrio versutus strain D301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474339..485202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TVD_RS02235 (TVD_02235) argJ 475656..476867 (+) 1212 WP_047250711.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  TVD_RS02240 (TVD_02240) - 476848..477822 (+) 975 WP_047250712.1 Nudix family hydrolase -
  TVD_RS02245 (TVD_02245) zapD 477855..478625 (-) 771 WP_018939032.1 cell division protein ZapD -
  TVD_RS02250 (TVD_02250) coaE 478685..479329 (-) 645 WP_019563203.1 dephospho-CoA kinase -
  TVD_RS02255 (TVD_02255) pilD 479339..480202 (-) 864 WP_047250713.1 prepilin peptidase Machinery gene
  TVD_RS02260 (TVD_02260) pilC 480206..481417 (-) 1212 WP_018144450.1 type II secretion system F family protein Machinery gene
  TVD_RS02265 (TVD_02265) pilB 481461..483179 (-) 1719 WP_047250714.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TVD_RS02285 (TVD_02285) tuf 483800..484990 (+) 1191 WP_018168213.1 elongation factor Tu -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31062.91 Da        Isoelectric Point: 7.7019

>NTDB_id=145152 TVD_RS02255 WP_047250713.1 479339..480202(-) (pilD) [Thioalkalivibrio versutus strain D301]
MLAEIGAQLGGWALPVAGVFGLLIGSFLNVVIARLPVMLEREWETEARAILEHAPNDERVRFDLIRPRSRCPQCQRPIRA
LENVPILSFLFLRGRCPGCGTRISWQYPAVEALTALLFVVTVWHLGPGSAGLLALVFTAVLIAASGIDARTTLLPDQLTL
PLLWLGLLVNIPGTFTDLQSAVIGAAAGYLTLWLIFHGFRLLTGKEGMGFGDFKLLAALGAWLGWQALPVILLLASLVGA
VVGIALILLRGRDRNIPIPFGPYLAAAGWLALIWGDELLALYFPQGF

Nucleotide


Download         Length: 864 bp        

>NTDB_id=145152 TVD_RS02255 WP_047250713.1 479339..480202(-) (pilD) [Thioalkalivibrio versutus strain D301]
ATGCTGGCCGAGATCGGAGCCCAACTGGGCGGCTGGGCACTGCCGGTTGCCGGGGTGTTCGGGCTGCTGATCGGCAGCTT
CCTGAACGTGGTGATCGCACGCCTCCCGGTGATGCTGGAACGCGAATGGGAGACCGAGGCGCGCGCGATTCTCGAGCACG
CGCCGAACGACGAGCGCGTGCGATTCGACCTGATCCGCCCGCGCTCGCGCTGCCCGCAATGCCAGCGCCCGATCCGGGCG
CTGGAAAACGTGCCCATTCTCAGCTTCCTGTTCCTGCGCGGTCGCTGCCCCGGGTGTGGCACCCGCATCAGTTGGCAGTA
TCCCGCAGTGGAGGCACTCACCGCGCTGCTCTTCGTGGTAACGGTCTGGCACCTGGGGCCCGGTAGCGCCGGGCTACTGG
CGCTGGTCTTCACGGCCGTACTGATCGCCGCCTCCGGGATCGATGCCCGGACGACGCTACTCCCCGATCAGCTCACCCTG
CCGCTTTTGTGGCTCGGGCTGCTGGTCAACATCCCCGGTACCTTCACCGACCTGCAGTCCGCCGTGATCGGTGCTGCGGC
CGGTTACCTGACCTTGTGGCTGATCTTCCACGGCTTCCGCCTGCTGACCGGCAAGGAAGGCATGGGCTTCGGTGACTTCA
AACTGCTGGCGGCGCTGGGCGCGTGGCTCGGCTGGCAGGCACTGCCGGTCATCCTGCTACTGGCCTCACTGGTCGGCGCG
GTGGTCGGGATCGCGCTGATCCTGCTGCGCGGGCGCGATCGCAACATCCCGATCCCATTCGGGCCCTATCTCGCCGCTGC
CGGGTGGCTCGCCTTGATCTGGGGCGACGAGCTGCTGGCCCTCTATTTCCCGCAAGGGTTTTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G3FZB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.818

95.819

0.516
  pilD Neisseria gonorrhoeae MS11

50.185

94.425

0.474
  pilD Vibrio campbellii strain DS40M4

50.186

93.728

0.47
  pilD Acinetobacter baumannii D1279779

50.57

91.638

0.463
  pilD Acinetobacter nosocomialis M2

49.81

91.638

0.456

Multiple sequence alignment