Detailed information NTG0144871

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AAV29_RS12970
• Genome accession: NZ_CP011346
• Coordinates: 2683724..2684338 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ-D34
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2678724..2689338

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAV29_RS12945 (AAV29_12945)	rpsT	2679024..2679290 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AAV29_RS12950 (AAV29_12950)	holA	2679307..2680348 (-)	1042	Protein_2580	DNA polymerase III subunit delta	-
AAV29_RS21130	-	2680388..2680542 (-)	155	Protein_2581	hypothetical protein	-
AAV29_RS20670 (AAV29_12955)	-	2680583..2680717 (+)	135	WP_003152870.1	YqzM family protein	-
AAV29_RS12960 (AAV29_12960)	comEC	2680736..2683087 (-)	2352	WP_046559887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAV29_RS12965 (AAV29_12965)	-	2683088..2683657 (-)	570	WP_003152868.1	ComE operon protein 2	-
AAV29_RS12970 (AAV29_12970)	comEA	2683724..2684338 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAV29_RS12975 (AAV29_12975)	comER	2684397..2685218 (+)	822	WP_014305449.1	late competence protein ComER	-
AAV29_RS12980 (AAV29_12980)	-	2685287..2686024 (-)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
AAV29_RS12985 (AAV29_12985)	rsfS	2686021..2686377 (-)	357	WP_003152864.1	ribosome silencing factor	-
AAV29_RS12990 (AAV29_12990)	yqeK	2686395..2686955 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAV29_RS12995 (AAV29_12995)	-	2686945..2687514 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
AAV29_RS13000 (AAV29_13000)	yhbY	2687525..2687815 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
AAV29_RS13005 (AAV29_13005)	aroE	2687809..2688651 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=144871 AAV29_RS12970 WP_041482377.1 2683724..2684338(-) (comEA) [Bacillus velezensis strain JJ-D34]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=144871 AAV29_RS12970 WP_041482377.1 2683724..2684338(-) (comEA) [Bacillus velezensis strain JJ-D34]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363