Detailed information NTG0144869

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: AAV29_RS12960
• Genome accession: NZ_CP011346
• Coordinates: 2680736..2683087 (-)
• Length: 783 a.a.
• NCBI ID: WP_046559887.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JJ-D34
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2675736..2688087

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAV29_RS12930 (AAV29_12930)	-	2676099..2676437 (-)	339	WP_003152882.1	YqxA family protein	-
AAV29_RS12935 (AAV29_12935)	spoIIP	2676454..2677647 (-)	1194	WP_014305446.1	stage II sporulation protein P	-
AAV29_RS12940 (AAV29_12940)	gpr	2677715..2678821 (-)	1107	WP_003152878.1	GPR endopeptidase	-
AAV29_RS12945 (AAV29_12945)	rpsT	2679024..2679290 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AAV29_RS12950 (AAV29_12950)	holA	2679307..2680348 (-)	1042	Protein_2580	DNA polymerase III subunit delta	-
AAV29_RS21130	-	2680388..2680542 (-)	155	Protein_2581	hypothetical protein	-
AAV29_RS20670 (AAV29_12955)	-	2680583..2680717 (+)	135	WP_003152870.1	YqzM family protein	-
AAV29_RS12960 (AAV29_12960)	comEC	2680736..2683087 (-)	2352	WP_046559887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAV29_RS12965 (AAV29_12965)	-	2683088..2683657 (-)	570	WP_003152868.1	ComE operon protein 2	-
AAV29_RS12970 (AAV29_12970)	comEA	2683724..2684338 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
AAV29_RS12975 (AAV29_12975)	comER	2684397..2685218 (+)	822	WP_014305449.1	late competence protein ComER	-
AAV29_RS12980 (AAV29_12980)	-	2685287..2686024 (-)	738	WP_032856885.1	class I SAM-dependent DNA methyltransferase	-
AAV29_RS12985 (AAV29_12985)	rsfS	2686021..2686377 (-)	357	WP_003152864.1	ribosome silencing factor	-
AAV29_RS12990 (AAV29_12990)	yqeK	2686395..2686955 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAV29_RS12995 (AAV29_12995)	-	2686945..2687514 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
AAV29_RS13000 (AAV29_13000)	yhbY	2687525..2687815 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86656.66 Da
• Isoelectric Point: 8.9037

>NTDB_id=144869 AAV29_RS12960 WP_046559887.1 2680736..2683087(-) (comEC) [Bacillus velezensis strain JJ-D34]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQPAPVVVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATIPGGFDYKEYLYSQQI
HWLFTVTSIQQCEKSKQPLFKLLSIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGMLLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YLLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVLKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGKSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFEKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=144869 AAV29_RS12960 WP_046559887.1 2680736..2683087(-) (comEC) [Bacillus velezensis strain JJ-D34]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCCTGCTCCGGTTGTTGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGATTCCCGGGGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTACCGTGACTTCTATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAGCATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAAGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGACTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGGATGTTGCTGCTGCT
GTTTTTGCCGGTCTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCTCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACCTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCCTCATTCATCGCAGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTTAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTAGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGCCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCGCTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAGGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAAGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACAGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCACGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTGAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.477	98.595	0.557