Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MOVS_RS09770 Genome accession   NZ_CP011158
Coordinates   2061437..2063092 (+) Length   551 a.a.
NCBI ID   WP_063514741.1    Uniprot ID   A0A378PMJ9
Organism   Moraxella ovis strain 199/55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2056437..2068092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOVS_RS09740 (MOVS_09650) nusA 2057549..2059030 (-) 1482 WP_063514738.1 transcription termination factor NusA -
  MOVS_RS09745 (MOVS_09655) rimP 2059079..2059576 (-) 498 WP_063514739.1 ribosome maturation factor RimP -
  MOVS_RS09760 (MOVS_09670) secG 2060112..2060438 (-) 327 WP_063514740.1 preprotein translocase subunit SecG -
  MOVS_RS09765 (MOVS_09675) tpiA 2060482..2061219 (-) 738 WP_063515049.1 triose-phosphate isomerase -
  MOVS_RS09770 (MOVS_09680) pilB 2061437..2063092 (+) 1656 WP_063514741.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MOVS_RS09775 (MOVS_09685) - 2063085..2064332 (+) 1248 WP_084260702.1 type II secretion system F family protein -
  MOVS_RS09780 (MOVS_09690) - 2064329..2065231 (+) 903 WP_063514742.1 A24 family peptidase -
  MOVS_RS09785 (MOVS_09695) coaE 2065275..2065913 (+) 639 WP_063514743.1 dephospho-CoA kinase -
  MOVS_RS09790 (MOVS_09700) - 2065910..2066465 (-) 556 Protein_1952 DUF924 family protein -
  MOVS_RS09795 (MOVS_09705) - 2066479..2067030 (-) 552 WP_084260703.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60259.69 Da        Isoelectric Point: 6.4631

>NTDB_id=143029 MOVS_RS09770 WP_063514741.1 2061437..2063092(+) (pilB) [Moraxella ovis strain 199/55]
MNHTTTGLARIILDKALINHDVLGAAIDDASAAQISLARHLVRHGVLSSDVIAKTLSESLGIDLVDLDKFNIKELPLHLL
DDALIARHEVLPLGQRDGQIFIATSDPTRTQAMSELGFATNAHITPVLVDENKLVRIMQSLHLGAVLELGEDDHELPDND
GETMLDAPTVKFVHKTLSDAIIMGASDIHFEPFDKHYRVRFRIDGIMQTIANPPKASGNKISTRLKVMARLDIAERRKPQ
DGRIKFPLHEGKSVDFRVSTTPTLFGEKIVLRLLDSAQALIGIDTLGMNDAQKQLFLDALNRPQGMIVITGPTGSGKTIS
LYTALGILNRPEINIQTVEDPVEIHLDGINQVNINPKIGLDFADVLRAFLRQDPDVIMVGEIRDAQTAEIAIKAAQTGHL
VLSTLHTNSAIDTIIRLKNMGVATFNIASSLSLIIAQRLVRRLCGDCKQPLDIPAQSLIELGFSPDQIGTAKLYKPVGCH
QCKDGYKGRIGIYEMLPITPKLATLIMSDAPTQALSEFAKEHGMMTLRQSAMHQVISGITSLQEMERLTGA

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=143029 MOVS_RS09770 WP_063514741.1 2061437..2063092(+) (pilB) [Moraxella ovis strain 199/55]
ATGAACCACACCACCACAGGACTTGCTCGAATTATACTAGATAAAGCACTCATCAATCATGACGTGCTTGGGGCTGCCAT
TGATGACGCTAGTGCCGCGCAGATTAGCCTTGCTCGCCATCTAGTGCGACATGGCGTACTGTCCTCCGATGTGATCGCCA
AGACTTTATCTGAGAGTCTGGGCATAGACTTGGTGGATTTGGATAAATTTAACATCAAAGAGCTGCCCTTGCATCTGCTT
GATGATGCGCTCATTGCACGCCACGAGGTTTTACCCCTTGGTCAGCGCGATGGGCAAATTTTTATCGCCACCAGTGACCC
GACACGCACGCAAGCAATGAGCGAGCTTGGCTTTGCAACCAATGCGCACATCACGCCCGTTCTGGTCGATGAGAATAAAC
TCGTACGCATCATGCAAAGCCTGCATCTAGGCGCGGTGCTAGAGCTTGGCGAGGACGATCATGAACTGCCCGATAATGAC
GGTGAGACAATGCTTGATGCGCCGACCGTTAAATTCGTCCACAAGACATTAAGTGATGCCATCATCATGGGTGCATCAGA
CATTCATTTTGAACCGTTCGATAAGCATTATCGGGTAAGATTTCGCATTGATGGCATCATGCAGACCATCGCAAACCCTC
CCAAGGCTTCAGGCAATAAAATCAGTACCCGCCTAAAAGTCATGGCACGTCTAGACATTGCCGAACGACGAAAGCCGCAA
GATGGACGCATCAAATTTCCCCTGCATGAAGGCAAATCGGTTGATTTTCGTGTCAGTACCACGCCCACACTCTTTGGCGA
GAAGATCGTCTTAAGGCTACTAGATTCTGCCCAAGCCCTAATCGGCATTGATACGCTCGGCATGAATGATGCCCAAAAAC
AGCTGTTCCTTGATGCACTAAACCGTCCTCAAGGCATGATCGTCATCACAGGCCCGACAGGCTCTGGTAAGACGATCTCG
CTATACACCGCGCTTGGCATCCTAAATCGACCCGAGATTAACATCCAAACCGTCGAAGACCCTGTGGAAATTCACCTAGA
TGGCATCAATCAGGTCAATATCAACCCAAAGATTGGGCTTGACTTTGCTGATGTGCTAAGAGCTTTCTTGCGTCAAGATC
CCGACGTCATCATGGTGGGCGAGATTCGCGACGCACAGACCGCAGAGATTGCCATTAAGGCGGCTCAGACAGGCCACTTG
GTGCTGTCCACATTACACACCAATTCTGCCATCGACACCATCATTCGCCTAAAGAACATGGGCGTGGCAACCTTTAATAT
CGCAAGCTCTCTAAGTCTCATCATCGCCCAAAGGCTCGTCCGCCGTCTGTGCGGTGACTGTAAGCAGCCCTTAGACATCC
CTGCCCAAAGCCTGATTGAGCTTGGCTTTAGCCCAGATCAAATCGGCACAGCCAAGCTATATAAGCCCGTCGGTTGCCAT
CAATGCAAAGACGGCTACAAAGGACGCATAGGCATCTATGAGATGCTGCCCATCACCCCTAAGCTCGCCACGCTCATCAT
GAGCGACGCGCCGACTCAGGCACTTAGCGAATTTGCCAAAGAACACGGCATGATGACCCTTAGACAATCTGCCATGCATC
AGGTCATCAGCGGCATCACAAGCCTACAAGAGATGGAGCGTCTCACAGGTGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-139)

Type II/IV secretion system protein (T2SSE)

(175-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378PMJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

48.319

100

0.495
  pilB Acinetobacter baylyi ADP1

48.128

100

0.49
  pilB Legionella pneumophila strain ERS1305867

49.907

97.822

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

100

0.486
  pilB Vibrio campbellii strain DS40M4

46.797

100

0.477
  pilB Vibrio cholerae strain A1552

46.702

100

0.475
  pilF Neisseria gonorrhoeae MS11

44.485

100

0.446
  pilF Thermus thermophilus HB27

37.138

100

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.977

95.1

0.361

Multiple sequence alignment