Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PCL1606_RS27495 Genome accession   NZ_CP011110
Coordinates   6134999..6137056 (-) Length   685 a.a.
NCBI ID   WP_045886204.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain PCL1606     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6129999..6142056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCL1606_RS27480 (PCL1606_56310) - 6131628..6133217 (-) 1590 WP_045886201.1 SPOR domain-containing protein -
  PCL1606_RS27485 (PCL1606_56320) aroB 6133228..6134328 (-) 1101 WP_044460778.1 3-dehydroquinate synthase -
  PCL1606_RS27490 (PCL1606_56330) aroK 6134476..6134994 (-) 519 WP_044460777.1 shikimate kinase AroK -
  PCL1606_RS27495 (PCL1606_56340) pilQ 6134999..6137056 (-) 2058 WP_045886204.1 type IV pilus secretin PilQ Machinery gene
  PCL1606_RS27500 (PCL1606_56350) - 6137070..6137597 (-) 528 WP_045886207.1 pilus assembly protein PilP -
  PCL1606_RS27505 (PCL1606_56360) - 6137594..6138211 (-) 618 WP_045886208.1 type 4a pilus biogenesis protein PilO -
  PCL1606_RS27510 (PCL1606_56370) - 6138208..6138774 (-) 567 WP_045886209.1 PilN domain-containing protein -
  PCL1606_RS27515 (PCL1606_56380) comM 6138774..6139826 (-) 1053 WP_045886213.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 685 a.a.        Molecular weight: 74470.60 Da        Isoelectric Point: 5.3090

>NTDB_id=142340 PCL1606_RS27495 WP_045886204.1 6134999..6137056(-) (pilQ) [Pseudomonas chlororaphis strain PCL1606]
MNRIFSALGIALGMALLSPMAMAATLKALDVATLSGDRLELKLTFDGPVPEPRGYSTDQPARIALDLPDVVSGLKSKSRD
LGSGNARSVTVVESGQRTRVVVNLLALAPYSTRIVGNQLFVVIGQGATVAHDVPQRPAASGRVGAGRSIRSVDFQRGERG
EGNVLIDLSDAQMSPDIQERDGKLVIAFARARLPEPLRVRLDVKDFATPVQFVSARAESDKAIITIEPGGTFDYSTYQTD
NKLTVSVRPMSVDDLQKRNAERSTYDGEKLSLNFQDIEVRSVLQLIADFTHLNLVASDTVQGGITLRLQNVPWDQALDLV
LKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDERGSITV
DERTNNIIAYQTRDRLDELRRIVSQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGANGSSTIG
TPGSTSSNAPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAASSG
ATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQSKVVD
KVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2058 bp        

>NTDB_id=142340 PCL1606_RS27495 WP_045886204.1 6134999..6137056(-) (pilQ) [Pseudomonas chlororaphis strain PCL1606]
ATGAACAGGATCTTTTCAGCCCTCGGTATTGCGCTAGGGATGGCGCTGCTTTCGCCGATGGCCATGGCGGCCACTCTCAA
GGCCCTGGATGTCGCGACGCTGTCGGGAGACCGTCTGGAGTTGAAGCTGACCTTCGATGGGCCGGTTCCCGAGCCCCGTG
GCTATAGCACCGACCAGCCGGCACGGATCGCCCTCGACTTGCCGGATGTCGTCAGTGGGCTCAAGAGCAAAAGCCGTGAC
CTGGGGAGCGGCAATGCGCGCAGCGTCACCGTGGTCGAGTCGGGGCAGCGCACCCGTGTGGTGGTCAATCTGCTGGCGCT
GGCGCCCTATAGCACCCGGATCGTGGGGAACCAGCTGTTCGTGGTGATCGGTCAGGGCGCGACGGTGGCGCACGATGTGC
CGCAGCGTCCTGCTGCCTCCGGACGAGTCGGGGCGGGCCGGTCGATTCGCAGCGTGGATTTTCAGCGGGGGGAGCGGGGT
GAAGGCAATGTCCTGATCGACCTTTCCGATGCGCAGATGAGCCCCGATATCCAGGAGCGCGACGGCAAGCTGGTGATCGC
CTTTGCCCGGGCCCGCCTGCCCGAACCCTTGCGCGTCAGGCTGGACGTCAAGGATTTCGCTACGCCTGTGCAGTTTGTCA
GCGCCCGCGCGGAATCGGACAAGGCCATCATTACCATCGAGCCCGGTGGCACCTTCGATTACTCGACCTACCAGACGGAC
AACAAATTGACGGTCAGCGTCAGGCCGATGAGTGTTGACGACCTGCAGAAACGTAATGCCGAGCGCTCGACCTATGACGG
TGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTACTGCAACTGATTGCCGACTTCACCCATCTCAACC
TGGTGGCCAGCGATACGGTCCAGGGCGGGATTACCCTGCGTCTGCAGAATGTGCCGTGGGACCAGGCCCTGGACCTGGTG
CTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTAGTGGCGCCGGCCGATGAAATCGCCGCTCGTGA
ACGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGATCTCGAGCCGTTGCGCCGCGAACTGTTGCAGGTCAATTACGCCA
AGGCTGCGGAGATCGCCAAGCTGTTCCAGTCAGTCACCCGCGCCGAGGATAAATCCGATGAGCGTGGTTCGATCACGGTC
GATGAGCGGACCAACAACATCATTGCCTATCAGACCCGGGATCGCCTGGACGAACTGCGGCGCATCGTCAGCCAGCTGGA
TGTTCCGGTACGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCGAACGTCGACTATGACAAGAGCCTGGGCGTGCGCT
GGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAATGGCGCCAATGGTTCCTCCACCATCGGC
ACGCCGGGCAGCACCAGCAGTAATGCACCGTTCGTCGACTTGGGCACTACCGCCAATACCTCCGGGCTGGGGATCGCCTT
CATCACCGACAACGTGTTGCTGGACCTTGAGCTGACGGCGATGGAAAAGACCGGCAACGGGGAAATCGTCTCCCAGCCCA
AGGTCGTCACTTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACGGAAATCCCCTATCAGGAAGCCGCGTCCAGCGGG
GCGACCTCGGTGTCTTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACGCCGCAGATCACTCCGGACAACCGCATCATCAT
GGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGATGTACCGCCCATCAAGAAGAACGAGGTCA
ACGCCAAGGTCCTGATCAACGACGGAGAGACCATCGTGATCGGTGGGGTTTTCTCCAATACGCAAAGCAAGGTTGTAGAT
AAAGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGAGAAAAAATCCGAGCT
GCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(367-432)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(293-339)

Bacterial type II and III secretion system protein (Secretin)

(520-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.371

100

0.724
  pilQ Acinetobacter baumannii strain A118

37.772

100

0.406
  pilQ Acinetobacter baumannii D1279779

37.772

100

0.406
  comQ Acinetobacter baylyi ADP1

40.348

100

0.406
  pilQ Legionella pneumophila strain Lp02

38.187

98.248

0.375
  pilQ Legionella pneumophila strain ERS1305867

38.152

97.956

0.374

Multiple sequence alignment