Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PNIG_RS02135 Genome accession   NZ_CP011036
Coordinates   460899..462572 (-) Length   557 a.a.
NCBI ID   WP_089367690.1    Uniprot ID   A0AAC9UFL1
Organism   Pseudoalteromonas nigrifaciens strain KMM 661     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455899..467572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNIG_RS02115 (PNIG_a0463) - 456722..458137 (-) 1416 WP_089367688.1 ATP-binding protein -
  PNIG_RS02120 (PNIG_a0464) - 458115..458804 (-) 690 WP_089367689.1 response regulator transcription factor -
  PNIG_RS02125 (PNIG_a0465) - 458969..459454 (+) 486 WP_086996570.1 Spy/CpxP family protein refolding chaperone -
  PNIG_RS02130 (PNIG_a0467) yacG 459996..460226 (-) 231 WP_011327088.1 DNA gyrase inhibitor YacG -
  PNIG_RS02135 (PNIG_a0468) pilB 460899..462572 (-) 1674 WP_089367690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PNIG_RS02140 (PNIG_a0469) coaE 462693..463325 (-) 633 WP_089367691.1 dephospho-CoA kinase -
  PNIG_RS02145 (PNIG_a0470) pilD 463327..464247 (-) 921 WP_089367692.1 A24 family peptidase Machinery gene
  PNIG_RS02150 (PNIG_a0471) - 464705..465088 (-) 384 WP_089367693.1 GxxExxY protein -
  PNIG_RS02155 (PNIG_a0472) pilC 465749..466966 (-) 1218 WP_089367694.1 type II secretion system F family protein Machinery gene
  PNIG_RS20250 (PNIG_a0473) - 466977..467417 (-) 441 WP_089367695.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62069.63 Da        Isoelectric Point: 6.2146

>NTDB_id=141653 PNIG_RS02135 WP_089367690.1 460899..462572(-) (pilB) [Pseudoalteromonas nigrifaciens strain KMM 661]
MNINSPLLRKFITLGRVDADIVKTKQQEFASTAELIAKCGKIESKDLAAQCIDLFRVPFFDLKDFNTALISPELIKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDKKIEQLLDATGSLNLSDDEFKEFAELDADI
QKDTSKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDIA
EKRKPQDGRIKLKITERKSVDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPTG
SGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIKA
AQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPENLPSEELLRQGFSQQQISEMTLY
APKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=141653 PNIG_RS02135 WP_089367690.1 460899..462572(-) (pilB) [Pseudoalteromonas nigrifaciens strain KMM 661]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTTGGCCGTGTTGATGCCGATATAGTAAAAACTAAACAGCA
AGAGTTTGCCTCTACTGCTGAGCTTATTGCTAAATGTGGAAAAATTGAAAGTAAAGATTTAGCGGCGCAATGCATTGATT
TATTTAGGGTGCCTTTTTTTGATTTAAAAGACTTTAATACTGCACTTATTTCTCCTGAGTTAATAAAAGAAAAACTGATC
CGTAAGCATCATATTCTGCCTTTGGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCTGATCCTACCGATTACGGCGC
ATTTGAAAACTTTGAATTTAGTACTGGTTTGTCTTGCGAAGTGGTAGTGGTTGACTACATTCAACTCGATAAAAAAATAG
AACAATTGCTTGATGCCACTGGCAGCTTAAATTTAAGTGATGACGAGTTTAAAGAATTTGCCGAACTAGACGCCGATATA
CAAAAAGACACCTCTAAAGATGATGATAAAGACGATGCACCAATCATTGTTTACATTAATAAAATATTAATGGATGCGAT
TAAAAAAGGTGCGTCTGATTTACACTTTGAACCCTACGAGCACAAATACCGTATTCGCTTTAGAATTGACGGCATTTTAC
ACGAAGTAGCCAGCCCACCAAACACTTTAGCAACTAAGCTTTCGGCGCGTATTAAAGTAATGTCGCGCCTTGATATTGCC
GAAAAACGTAAACCGCAAGATGGCCGCATAAAGCTAAAAATAACCGAACGTAAAAGTGTCGACTTTCGGGTAAGCACCAT
GCCCACTTTATGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCTAGCGCCATGCTTGGCATTGATGTATTAGGTT
ACGAACCCGAGCAAAAAAAGCTGTATATGGATGCGCTTGAGCAACCACAAGGGATGATATTAGTAACTGGGCCTACTGGC
TCGGGTAAAACGGTATCGCTTTACACCGGCTTAAATATATTAAACCAGCCTGAGCGTAATATTAGTACCGCCGAAGATCC
GGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATTAATATAAAAGCCGATATGACCTTTGCCAACGCACTACGGG
CATTTTTACGCCAAGATCCCGATGTAGTAATGGTAGGTGAAATACGTGACCTAGAAACCGCTGAAATATCAATTAAAGCG
GCGCAAACAGGTCACTTAGTGCTTTCGACCTTGCATACTAACTCGGCGCCCGAAACCATTACCCGCTTATTAAACATGGG
CGTACCGGCATATAACGTAGCAAGTTCAATTAGCTTAATTATAGCCCAGCGCCTAGCGCGCCGCTTATGCCCTAAATGTA
AAACGCCTGAAAATTTACCTAGTGAAGAGCTTCTACGCCAAGGTTTTAGCCAGCAGCAAATTAGTGAAATGACCTTGTAT
GCTCCTAAAGGTTGCGAAAACTGTACCGATGGTTATAAAGGTCGTGTAGGTATTTACGAAGTAATGAAAATTACCCCAGA
AATAGCACAAATTATAATGCGCGGTGGTAACTCACTAGAAATAGCCGAAGTGTCGCTTAAAGCCGGATTTAATAACCTGC
GTTTATCGGGCATTCGTAAAGCCGCCGACGGCATGACCTCGTTAGCTGAGATTAATCGGGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)

Type II/IV secretion system protein (T2SSE)

(181-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.541

93.537

0.548
  pilB Acinetobacter baylyi ADP1

58.915

92.639

0.546
  pilB Legionella pneumophila strain ERS1305867

52.919

95.332

0.504
  pilB Vibrio cholerae strain A1552

52.158

95.691

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

95.691

0.497
  pilB Vibrio campbellii strain DS40M4

51.311

95.871

0.492
  pilF Neisseria gonorrhoeae MS11

49.712

93.537

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

37.359

100

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.77

99.82

0.377

Multiple sequence alignment