Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PARC_RS15525 Genome accession   NZ_CP011025
Coordinates   3424996..3426672 (+) Length   558 a.a.
NCBI ID   WP_010553355.1    Uniprot ID   A0A290S6S5
Organism   Pseudoalteromonas arctica A 37-1-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3419996..3431672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PARC_RS15500 (PARC_a3513) nadC 3420190..3421041 (+) 852 WP_010553350.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PARC_RS15505 (PARC_a3514) - 3421534..3421980 (+) 447 WP_010553351.1 pilin -
  PARC_RS15510 (PARC_a3515) pilC 3422046..3423269 (+) 1224 WP_010553352.1 type II secretion system F family protein Machinery gene
  PARC_RS15515 (PARC_a3516) pilD 3423333..3424226 (+) 894 WP_010553353.1 A24 family peptidase Machinery gene
  PARC_RS15520 (PARC_a3518) coaE 3424228..3424869 (+) 642 WP_010553354.1 dephospho-CoA kinase -
  PARC_RS15525 (PARC_a3519) pilB 3424996..3426672 (+) 1677 WP_010553355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PARC_RS15530 (PARC_a3520) yacG 3426787..3427017 (+) 231 WP_007586146.1 DNA gyrase inhibitor YacG -
  PARC_RS15535 (PARC_a3521) - 3427089..3427577 (-) 489 WP_010553356.1 periplasmic heavy metal sensor -
  PARC_RS15540 (PARC_a3522) - 3427755..3428444 (+) 690 WP_007586150.1 response regulator -
  PARC_RS15545 (PARC_a3523) - 3428422..3429837 (+) 1416 WP_010553357.1 ATP-binding protein -
  PARC_RS15550 (PARC_a3524) - 3429841..3430788 (+) 948 WP_010553358.1 alpha/beta fold hydrolase -
  PARC_RS15555 (PARC_a3525) - 3430748..3431614 (-) 867 WP_021032111.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62199.42 Da        Isoelectric Point: 5.3421

>NTDB_id=141557 PARC_RS15525 WP_010553355.1 3424996..3426672(+) (pilB) [Pseudoalteromonas arctica A 37-1-2]
MNINSPLLRKFITLGRIDADTVKNKQNEFASTAELISKCGKIDSKDLAEQCIDLFRVPYFDLKDFDPTKIPADLVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDTKIEQLLDATGSLNLTDDEFQEFADLDSES
QRDTAPQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEQLPSEELARQGFTAKQIEEMTL
YTPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIADVSLKAGFNNLRLSGLRKAADGLTSLGEINRVTNF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=141557 PARC_RS15525 WP_010553355.1 3424996..3426672(+) (pilB) [Pseudoalteromonas arctica A 37-1-2]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACCCTTGGCCGTATTGATGCCGACACGGTTAAAAACAAACAAAA
TGAATTTGCCTCAACAGCTGAGCTTATTTCTAAATGTGGAAAAATCGATAGTAAAGATTTAGCTGAACAATGTATTGATT
TGTTTCGCGTACCTTATTTTGATTTAAAAGATTTCGACCCTACAAAAATCCCGGCCGATCTTGTAAAAGAAAAACTCATA
CGTAAACATCACATTTTGCCGCTTGTTCAAAAAGATCGAAAAGTTTATATAGCAGCATCAGACCCAACAGATTACGGCGC
ATTCGAAAACTTTGAGTTTAGTACCGGTCTTTCATGCGAAATTGTTGTTGTTGATTATATTCAACTCGATACCAAAATCG
AGCAATTACTAGATGCTACTGGCAGCTTAAATTTAACTGATGACGAGTTTCAGGAATTTGCTGATTTAGACTCAGAAAGC
CAAAGAGATACAGCACCACAAGATGACGATAAAGATGATGCACCTATCATCGTTTACATCAACAAAATTTTAATGGATGC
CATTAAAAAAGGTGCCTCTGATTTACACTTTGAACCGTATGAGCACAAGTACCGTATCCGCTTTCGTATTGATGGCATTT
TGCATGAAGTGGCAAGCCCACCTAATACATTAGCAACCAAGCTTTCTGCACGTATTAAAGTAATGTCGCGACTCGATATT
GCCGAAAAACGCAAACCACAAGATGGTCGTATAAAACTAAAAATTACTGAACGCAAAAGTATCGACTTTCGTGTAAGTAC
TATGCCTACCTTGTGGGGCGAAAAAATAGTAATGCGTATCCTTGATTCATCAAGCGCTATGTTAGGCATTGATGTATTAG
GCTATGAGCCTGAGCAGAAAAAGCTCTATATGGATGCACTTGCCCAGCCTCAAGGGATGATATTAGTAACAGGGCCAACG
GGTTCTGGTAAAACAGTATCGCTATACACAGGTTTAAATATATTAAACCAGCCAGAGCGCAATATTAGTACTGCGGAAGA
TCCGGTTGAGATTAACCTTGAAGGTGTAAACCAAGTACAAATTAACATTAAAGCCGATATGACTTTTGCCAATGCACTGC
GTGCATTTTTACGTCAAGATCCTGATGTAGTTATGGTAGGTGAAATACGTGACCTAGAAACCGCAGAAATATCAATAAAA
GCGGCGCAAACAGGTCACTTGGTTTTATCAACATTGCATACAAACTCAGCGCCGGAAACAATCACTCGTTTATTAAATAT
GGGTGTGCCGGCTTATAACGTAGCAAGTTCAATCAGCTTAATAATAGCCCAACGTCTAGCACGTCGCTTGTGCCCTAAAT
GTAAAACGCCAGAACAACTTCCAAGTGAAGAACTGGCTCGCCAAGGATTTACCGCCAAGCAAATTGAAGAAATGACGTTA
TATACGCCTAAAGGGTGTGATAGCTGTACTGATGGTTACAAAGGGCGTGTGGGTATTTACGAAGTAATGCAAATTACTCC
TGAAATTGCCCAAATTATTATGCGCGGTGGTAACTCGCTTGAAATAGCAGACGTATCACTTAAAGCCGGATTTAATAATT
TACGTTTATCGGGTTTACGCAAAGCAGCCGATGGCCTTACATCCCTTGGTGAAATTAATCGCGTAACTAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A290S6S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.064

91.935

0.543
  pilB Acinetobacter baylyi ADP1

59.01

90.502

0.534
  pilB Legionella pneumophila strain ERS1305867

52.612

96.057

0.505
  pilB Vibrio cholerae strain A1552

52.336

95.878

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

51.498

95.699

0.493
  pilB Vibrio campbellii strain DS40M4

50.936

95.699

0.487
  pilF Neisseria gonorrhoeae MS11

47.491

100

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

37.138

100

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.273

98.566

0.367

Multiple sequence alignment