Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B723_RS07475 Genome accession   NZ_CP010945
Coordinates   1616945..1619038 (+) Length   697 a.a.
NCBI ID   WP_017336124.1    Uniprot ID   -
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1611945..1624038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS07455 (B723_07475) comM 1614157..1615221 (+) 1065 WP_017336120.1 pilus assembly protein PilM Machinery gene
  B723_RS07460 (B723_07480) - 1615221..1615787 (+) 567 WP_017336121.1 PilN domain-containing protein -
  B723_RS07465 (B723_07485) pilO 1615784..1616407 (+) 624 WP_017336122.1 type 4a pilus biogenesis protein PilO -
  B723_RS07470 (B723_07490) - 1616404..1616931 (+) 528 WP_017336123.1 pilus assembly protein PilP -
  B723_RS07475 (B723_07495) pilQ 1616945..1619038 (+) 2094 WP_017336124.1 type IV pilus secretin PilQ Machinery gene
  B723_RS07480 (B723_07500) aroK 1619043..1619561 (+) 519 WP_017336125.1 shikimate kinase AroK -
  B723_RS07485 (B723_07505) aroB 1619713..1620813 (+) 1101 WP_017336126.1 3-dehydroquinate synthase -
  B723_RS07490 (B723_07510) - 1620824..1622422 (+) 1599 WP_017336127.1 AAA family ATPase -

Sequence

Protein


Download         Length: 697 a.a.        Molecular weight: 75321.53 Da        Isoelectric Point: 5.5570

>NTDB_id=140802 B723_RS07475 WP_017336124.1 1616945..1619038(+) (pilQ) [Pseudomonas fluorescens NCIMB 11764]
MNRIFATFGVSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGAPPAPHGYTTEQPARIALDLPGVVSQLTSKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYTARVEGNNLFVVVGRGTSNAAPAPATASKPPRVATATPAPAKAYAPVSKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGRIVVGFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEP
SGAFDYSTYQTDNKLTVSVRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSG
VSNGANASSTIGTPGSTSTNSPFVDMGTAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKG
TEIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIG
GVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2094 bp        

>NTDB_id=140802 B723_RS07475 WP_017336124.1 1616945..1619038(+) (pilQ) [Pseudomonas fluorescens NCIMB 11764]
ATGAACAGGATTTTCGCAACCTTCGGAGTTTCGCTATGGATAGCGTTGCTTTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTCGCTGCGCTGCCGGGAGACCGCGTCGAGTTGAAGCTGTCATTCGATGGCGCGCCCCCAGCGCCCCACG
GTTACACGACGGAGCAGCCTGCGCGGATCGCGCTGGACCTGCCTGGTGTGGTCAGCCAGCTAACGAGCAAGACTCGCGAC
CTGGGCGGTGGCAATGCCCGCAGTGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCAACCTGACTCAATT
GACCCCGTACACCGCTCGTGTCGAAGGCAATAATCTGTTTGTGGTGGTCGGGCGCGGAACCAGTAACGCAGCACCTGCAC
CTGCAACCGCATCCAAGCCGCCACGCGTCGCCACGGCAACTCCCGCGCCGGCCAAGGCCTATGCGCCGGTGAGCAAGGCG
ATCCGCGGCGTGGATTTCCAGCGCGGCACCCAGGGTGAAGGCAATGTGGTCATCGATCTCTCGGATCCGTCCATCGCCCC
GGACATTCAGGAGCGCGATGGCAGGATTGTCGTCGGTTTCGCCAGGACCCAATTGCCGGAACCGTTGCGGGTCCGTCTGG
ACGTCAAGGATTTCGCCACGCCTGTGCAGTTCGTCAACGCCAGCGCTACGGGAGACCGGGCGACCATCAGCATTGAGCCC
AGCGGCGCGTTCGATTACTCGACCTATCAGACCGACAACAAGCTGACCGTCAGCGTCCGCCCGATGACCGTAGACGACTT
GCAGAAGCGCAACGCCGAGCGTTTTGCCTACACCGGTGAAAAGCTCTCGCTGAACTTCCAGGACATTGACGTGCGCTCGG
TGCTGCAACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATTACCTTGCGCTTG
CAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAACGAAAGATCGGTAACGTGTT
GCTGGTGGCGCCGGCCGATGAGATTGCCGCCCGCGAACGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGC
CTTTGCGTCGCGAGCTGCTGCAAGTCAATTACGCCAAGGCGGCCGATATCGCCAAGCTGTTCCAGTCAGTGACCAGTGCC
GAAGCGAAAGTCGACGAGCGGGGTTCGATCACCGTCGATGAACGAACCAATAACATCATCGCCTACCAGACCCAGGACCG
TCTCGACGAGCTGCGCCGTATTGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCTCGGATCGTCGAAG
CGAACGTCGACTACGACAAGAGCCTCGGTGTGCGTTGGGGTGGTTCGGTGCAGAACAAGGGCAACTGGAACACATCCGGT
GTCAGCAACGGCGCCAACGCGTCGTCGACCATTGGTACGCCGGGCAGCACCAGCACCAACTCACCGTTCGTCGACATGGG
CACCGCCGCCAACACTTCAGGGCTCGGCATCGCCTTCATCACCGATAATGTGCTGCTGGATCTTGAGCTGACCGCGATGG
AAAAAACCGGTAACGGCGAAATCGTCTCGCAACCCAAGGTGGTTACCTCCGACAAGGAAACCGCAAAAATCCTCAAGGGC
ACCGAGATCCCGTACCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTATCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGT
GACGCCGCAGATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGG
TGCAGGATGTACCGCCCATCAAGAAAAACGAGGTCAATGCCAAGGTTCTCGTCAATGACGGCGAGACCATCGTGATCGGT
GGGGTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTT
CCGGCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTG
CTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Bacterial type II and III secretion system protein (Secretin)

(532-688)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.045

100

0.75
  comQ Acinetobacter baylyi ADP1

41.738

100

0.42
  pilQ Acinetobacter baumannii strain A118

37.566

100

0.407
  pilQ Acinetobacter baumannii D1279779

37.566

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.529

97.561

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.529

97.561

0.376

Multiple sequence alignment