Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   B723_RS07455 Genome accession   NZ_CP010945
Coordinates   1614157..1615221 (+) Length   354 a.a.
NCBI ID   WP_017336120.1    Uniprot ID   A0A0K1QKG0
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1609157..1620221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS07445 (B723_07465) - 1610062..1611330 (+) 1269 WP_017336118.1 malic enzyme-like NAD(P)-binding protein -
  B723_RS07450 (B723_07470) - 1611501..1613939 (-) 2439 WP_031318294.1 penicillin-binding protein 1A -
  B723_RS07455 (B723_07475) comM 1614157..1615221 (+) 1065 WP_017336120.1 pilus assembly protein PilM Machinery gene
  B723_RS07460 (B723_07480) - 1615221..1615787 (+) 567 WP_017336121.1 PilN domain-containing protein -
  B723_RS07465 (B723_07485) pilO 1615784..1616407 (+) 624 WP_017336122.1 type 4a pilus biogenesis protein PilO -
  B723_RS07470 (B723_07490) - 1616404..1616931 (+) 528 WP_017336123.1 pilus assembly protein PilP -
  B723_RS07475 (B723_07495) pilQ 1616945..1619038 (+) 2094 WP_017336124.1 type IV pilus secretin PilQ Machinery gene
  B723_RS07480 (B723_07500) aroK 1619043..1619561 (+) 519 WP_017336125.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38083.53 Da        Isoelectric Point: 4.7245

>NTDB_id=140801 B723_RS07455 WP_017336120.1 1614157..1615221(+) (comM) [Pseudomonas fluorescens NCIMB 11764]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALTRVLLKARTSLRNVAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGASARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAGQLAAAQDRLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FSNMTLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=140801 B723_RS07455 WP_017336120.1 1614157..1615221(+) (comM) [Pseudomonas fluorescens NCIMB 11764]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGCTTTTGGGGATCGACATCAGCTCTACGTCGGTGAAGCTTCTGGAGTT
AAGCCGCCAGGGCGACCGCTACCGGGTCGAAGCCTATGCGGTAGAGCCGTTGCCTGCCAGCGCGGTGGTCGAGAAAAATA
TCGCCGAGCTCGAAGGCGTGGGTCAGGCACTCACGCGCGTGCTGCTCAAGGCCAGAACCAGTCTCAGGAACGTCGCAGTG
GCCGTTGCCGGCTCGGCGGTGATCACCAAGACCATCGAAATGGATGCCGGTCTTTCCGACGACGAGTTGGAAAACCAGCT
GAAAATCGAGGCTGATCAATACATCCCTTATCCGCTGGACGAAGTGGCCATCGACTTCGAAGTCCAGGGTGCATCGGCGC
GCAACCCCGAACGGGTCAATGTGTTGCTGGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAGGCGGCGCTCGCACTG
GCCGGCCTGACCGCTCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGATCCTTCGGTTTGCTGGCCGGACAGCTGGC
AGCCGCTCAGGACCGTCTGACGGTTGCCGTGGTCGACATCGGTGCGACCATGACCACGCTCAGCGTCCTGTACAACGGAC
GCATCATCTACACCCGCGAACAATTGTTCGGTGGTCGTCAACTGACGGAAGAAATCCAGCGACGTTATGGCCTGACCCTC
GAGCAAGCAGGGTTGGCAAAGAAGCAGGGCGGTTTGCCGGACGACTACGTCAGCGAGGTGCTGCAGCCATTTCGTGATGC
GCTGGTGCAGCAGGTGTCGCGATCGTTGCAGTTTTTCTTCGCCTCCGGGCAGTACAACGCTGTGGATCACATCCTGTTGG
CCGGTGGTACCGCATCGGTGCCGGGGCTGGACCGGTTGATCGAGCAGCGTTTGAGCACGCCGACGCAGGTGGCCAATCCC
TTCTCGAACATGACCCTGAGCAGCAAGGTCAACGCTGGCGCCCTGGCCAGCGATGCGCCGGCGCTGATGATCGCTTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QKG0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.732

100

0.489

Multiple sequence alignment