Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TO66_RS02190 Genome accession   NZ_CP010892
Coordinates   494221..496278 (+) Length   685 a.a.
NCBI ID   WP_044460776.1    Uniprot ID   -
Organism   Pseudomonas sp. MRSN 12121 strain MRSN12121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489221..501278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO66_RS02170 (TO66_02170) comM 491451..492503 (+) 1053 WP_044460772.1 pilus assembly protein PilM Machinery gene
  TO66_RS02175 (TO66_02175) - 492503..493069 (+) 567 WP_044460773.1 PilN domain-containing protein -
  TO66_RS02180 (TO66_02180) - 493066..493683 (+) 618 WP_044460774.1 type 4a pilus biogenesis protein PilO -
  TO66_RS02185 (TO66_02185) - 493680..494207 (+) 528 WP_044460775.1 pilus assembly protein PilP -
  TO66_RS02190 (TO66_02190) pilQ 494221..496278 (+) 2058 WP_044460776.1 type IV pilus secretin PilQ Machinery gene
  TO66_RS02195 (TO66_02195) aroK 496283..496801 (+) 519 WP_044460777.1 shikimate kinase AroK -
  TO66_RS02200 (TO66_02200) aroB 496949..498049 (+) 1101 WP_044460778.1 3-dehydroquinate synthase -
  TO66_RS02205 (TO66_02205) - 498060..499649 (+) 1590 WP_044460779.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 685 a.a.        Molecular weight: 74442.54 Da        Isoelectric Point: 5.3090

>NTDB_id=140662 TO66_RS02190 WP_044460776.1 494221..496278(+) (pilQ) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
MNRIFSALGIALGMALLSPMAMAATLKALDVATLSGDRLELKLTFDGPVPEPRGYSTDQPARIALDLPDVVSGLKSKSRD
LGSGNARSVTVVESGQRTRVVVNLLALAPYSTRIAGNQLFVVIGQGATVAHDVPQRPAASGRVGAGRSIRSVDFQRGERG
EGNVLIDLSDAQMSPDIQERDGKLVIAFARARLPEPLRVRLDVKDFATPVQFVSARAESDKAIITIEPGGTFDYSTYQTD
NKLTVSVRPMSVDDLQKRNAERSTYDGEKLSLNFQDIEVRSVLQLIADFTHLNLVASDTVQGGITLRLQNVPWDQALDLV
LKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDERGSITV
DERTNNIIAYQTRDRLDELRRIVSQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGANGSSTIG
TPGSTSSNAPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAASSG
ATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQSKVVD
KVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2058 bp        

>NTDB_id=140662 TO66_RS02190 WP_044460776.1 494221..496278(+) (pilQ) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
ATGAACAGGATCTTTTCAGCCCTCGGTATTGCGCTAGGGATGGCGCTGCTTTCGCCGATGGCCATGGCGGCCACTCTCAA
GGCCCTGGATGTCGCGACGCTGTCGGGAGACCGCCTGGAGTTGAAGCTGACCTTCGATGGGCCGGTTCCCGAGCCCCGTG
GCTATAGCACCGACCAGCCGGCACGGATCGCCCTCGACTTGCCGGATGTCGTCAGTGGGCTCAAGAGCAAAAGCCGTGAC
CTGGGGAGCGGCAATGCGCGCAGCGTCACCGTGGTCGAGTCGGGGCAGCGCACCCGTGTGGTGGTCAATCTGCTGGCGCT
GGCGCCCTATAGCACCCGGATCGCGGGGAACCAGCTGTTCGTGGTGATCGGTCAGGGCGCGACGGTGGCGCACGATGTGC
CGCAGCGTCCTGCTGCCTCCGGACGAGTCGGGGCGGGCCGGTCGATTCGCAGCGTGGATTTTCAGCGGGGGGAGCGGGGT
GAAGGCAATGTCCTGATCGACCTTTCCGATGCGCAGATGAGCCCCGATATCCAGGAGCGCGACGGCAAGCTGGTGATCGC
CTTTGCCCGGGCCCGCCTGCCCGAACCCTTGCGCGTCAGGCTGGACGTCAAGGATTTCGCTACGCCTGTGCAGTTTGTCA
GCGCCCGCGCGGAATCGGACAAGGCCATCATTACCATCGAGCCCGGTGGCACCTTCGATTACTCGACCTACCAGACGGAC
AACAAATTGACGGTCAGCGTCAGGCCGATGAGTGTTGACGACCTGCAGAAACGTAATGCCGAGCGCTCGACCTATGACGG
TGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTACTGCAACTGATTGCCGACTTCACCCATCTCAACC
TGGTGGCCAGCGATACGGTCCAGGGCGGGATTACCCTGCGTCTGCAGAATGTGCCGTGGGACCAGGCCCTGGACCTGGTG
CTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTGCTGCTAGTGGCGCCGGCCGATGAAATCGCCGCTCGTGA
ACGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGATCTCGAGCCGTTGCGCCGCGAACTGTTGCAGGTCAATTACGCCA
AGGCTGCGGAGATCGCCAAGCTGTTCCAGTCAGTCACCCGCGCCGAGGATAAATCCGATGAGCGTGGTTCGATCACGGTC
GATGAGCGGACCAACAACATCATTGCCTATCAGACCCGGGATCGCCTGGACGAACTGCGGCGCATCGTCAGCCAGCTGGA
TGTTCCGGTACGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCGAACGTCGACTATGACAAGAGCCTGGGCGTGCGCT
GGGGTGGCTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAATGGCGCCAATGGTTCCTCCACCATCGGC
ACGCCGGGCAGCACCAGCAGTAATGCACCGTTCGTCGACTTGGGCACTACCGCCAATACCTCCGGGCTGGGGATCGCCTT
CATCACCGACAACGTGTTGCTGGACCTTGAGCTGACGGCGATGGAAAAGACCGGCAACGGGGAAATCGTCTCCCAGCCCA
AGGTCGTCACTTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACGGAAATCCCCTATCAGGAAGCCGCGTCCAGCGGG
GCGACCTCGGTGTCTTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACGCCGCAGATCACTCCGGACAACCGCATCATCAT
GGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGATGTACCGCCCATCAAGAAGAACGAGGTCA
ACGCCAAGGTCCTGATCAACGACGGAGAGACCATCGTGATCGGTGGGGTTTTCTCCAATACGCAAAGCAAGGTTGTAGAT
AAAGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTGTCGGAGAAAAAATCCGAGCT
GCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(367-432)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(293-339)

Bacterial type II and III secretion system protein (Secretin)

(520-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.371

100

0.724
  pilQ Acinetobacter baumannii strain A118

37.772

100

0.406
  pilQ Acinetobacter baumannii D1279779

37.772

100

0.406
  comQ Acinetobacter baylyi ADP1

40.348

100

0.406
  pilQ Legionella pneumophila strain Lp02

38.336

98.248

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.301

97.956

0.375

Multiple sequence alignment