Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   TO66_RS02170 Genome accession   NZ_CP010892
Coordinates   491451..492503 (+) Length   350 a.a.
NCBI ID   WP_044460772.1    Uniprot ID   A0A0C5EDP7
Organism   Pseudomonas sp. MRSN 12121 strain MRSN12121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 486451..497503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO66_RS02160 (TO66_02160) - 487410..488678 (+) 1269 WP_044460770.1 malic enzyme-like NAD(P)-binding protein -
  TO66_RS02165 (TO66_02165) - 488800..491235 (-) 2436 WP_177330424.1 penicillin-binding protein 1A -
  TO66_RS02170 (TO66_02170) comM 491451..492503 (+) 1053 WP_044460772.1 pilus assembly protein PilM Machinery gene
  TO66_RS02175 (TO66_02175) - 492503..493069 (+) 567 WP_044460773.1 PilN domain-containing protein -
  TO66_RS02180 (TO66_02180) - 493066..493683 (+) 618 WP_044460774.1 type 4a pilus biogenesis protein PilO -
  TO66_RS02185 (TO66_02185) - 493680..494207 (+) 528 WP_044460775.1 pilus assembly protein PilP -
  TO66_RS02190 (TO66_02190) pilQ 494221..496278 (+) 2058 WP_044460776.1 type IV pilus secretin PilQ Machinery gene
  TO66_RS02195 (TO66_02195) aroK 496283..496801 (+) 519 WP_044460777.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 36895.42 Da        Isoelectric Point: 5.1049

>NTDB_id=140661 TO66_RS02170 WP_044460772.1 491451..492503(+) (comM) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
MLGLFSKKASSLLGIDISSTSVKLVELSRIGQRFQVEACGVEPLPANAVVEKNIVEPEGVGHALSRLLARTRPGVKNAAL
ALAGSAVISKVIEMDAGLSDDDRESQLKVEADQYIPYPLDEAALDFDVLGYSARHPERVDVLLAACRREHIEIREVALEL
AGLTPRVVDLETCALERSLALAAGPVASGSVALVDIGATLTTLNVVQAGQIIYSREQLFGGEQLTEQIQRRYGLSKEEAG
LAKKKGGLPDGYAQAVLQPFQETAVEQVSRSLQLFQASASQHRIGSLLLAGGSAGLPGLASLLQQRLGLPVRIANPFAGM
SLGSKVDAAVLASEAPALMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=140661 TO66_RS02170 WP_044460772.1 491451..492503(+) (comM) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
GTGCTAGGACTCTTCAGCAAAAAGGCGAGTTCCCTTCTCGGGATCGATATCAGTTCCACCTCGGTCAAGCTCGTTGAGTT
GAGCCGCATCGGCCAGCGTTTTCAGGTCGAGGCCTGCGGGGTGGAGCCGTTGCCGGCCAATGCCGTGGTCGAAAAGAACA
TCGTCGAGCCCGAAGGAGTGGGGCATGCCCTGTCCCGCCTGCTGGCCCGGACCCGGCCGGGTGTGAAGAACGCGGCGCTG
GCGCTGGCCGGTTCGGCGGTGATCAGCAAAGTCATCGAGATGGACGCCGGGCTGTCCGACGACGATCGGGAAAGCCAGCT
CAAGGTCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAGGCCGCCCTCGATTTCGACGTGCTCGGCTATTCGGCCC
GTCATCCCGAGCGTGTCGACGTCCTGCTGGCGGCCTGCCGCAGGGAGCACATTGAAATTCGCGAGGTGGCGCTGGAACTC
GCGGGGCTGACGCCCCGGGTGGTGGACCTGGAGACCTGCGCGCTCGAGCGCTCGCTCGCCCTGGCGGCCGGGCCGGTAGC
GTCGGGCTCCGTGGCGCTGGTCGATATCGGCGCCACCCTCACCACCCTGAACGTCGTGCAGGCAGGGCAAATCATCTATT
CCCGCGAGCAGTTGTTCGGCGGCGAACAACTGACCGAGCAGATCCAGCGCCGTTACGGCTTGTCGAAAGAGGAAGCGGGC
CTGGCGAAAAAAAAGGGCGGCTTGCCGGACGGCTATGCGCAAGCCGTGCTGCAACCTTTTCAAGAAACGGCGGTTGAGCA
GGTCTCACGTTCGTTGCAGTTGTTCCAGGCGTCGGCATCGCAGCACCGGATCGGCTCCCTGTTGTTGGCGGGTGGCAGCG
CCGGCCTGCCCGGGCTGGCCTCGCTGCTCCAGCAACGCCTGGGCCTGCCGGTACGGATCGCCAATCCCTTCGCCGGCATG
AGCCTGGGCAGCAAGGTCGATGCCGCAGTGCTGGCTAGCGAAGCGCCGGCCCTGATGATTGCCTGCGGATTGGCCCTGAG
GAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5EDP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

52.557

100

0.529
  pilM Acinetobacter baumannii D1279779

52.273

100

0.526
  comM Acinetobacter baylyi ADP1

50.284

100

0.506
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.457

Multiple sequence alignment