Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VAA049_RS02415 Genome accession   NZ_CP010811
Coordinates   493595..495283 (-) Length   562 a.a.
NCBI ID   WP_000957203.1    Uniprot ID   A0A5Q6PKK1
Organism   Vibrio cholerae strain 1154-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488595..500283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAA049_RS02390 (VAA049_466) yacG 489677..489874 (-) 198 WP_046121364.1 DNA gyrase inhibitor YacG -
  VAA049_RS02395 (VAA049_467) zapD 490016..490756 (-) 741 WP_000207194.1 cell division protein ZapD -
  VAA049_RS02400 (VAA049_468) coaE 490788..491396 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  VAA049_RS02405 (VAA049_469) pilD 491393..492268 (-) 876 WP_046121365.1 A24 family peptidase Machinery gene
  VAA049_RS02410 (VAA049_470) pilC 492326..493552 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  VAA049_RS02415 (VAA049_471) pilB 493595..495283 (-) 1689 WP_000957203.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VAA049_RS02420 (VAA049_472) - 495292..495756 (-) 465 WP_000649341.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VAA049_RS02425 (VAA049_473) nadC 496001..496891 (-) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VAA049_RS02430 (VAA049_474) ampD 497189..497734 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VAA049_RS02435 (VAA049_475) fldB 497885..498403 (-) 519 WP_000690112.1 flavodoxin FldB -
  VAA049_RS02440 (VAA049_476) xerD 498541..499449 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  VAA049_RS02445 (VAA049_477) dsbC 499466..500218 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62018.16 Da        Isoelectric Point: 6.2600

>NTDB_id=139937 VAA049_RS02415 WP_000957203.1 493595..495283(-) (pilB) [Vibrio cholerae strain 1154-74]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYAPLCKQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=139937 VAA049_RS02415 WP_000957203.1 493595..495283(-) (pilB) [Vibrio cholerae strain 1154-74]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGAACGTCGGTGCCTGAAGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAACTGACCGAACAACTGA
GCCATATTTTCGGTTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGTAAACAGCTTGGGCTGCGTGAA
CTGATAACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTCGCGGTCTCAGACCCGACCTTGCT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCATTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAAATCAGCCAAGATGAGCTAGCCACA
CTGGTCAAGGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGTATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTTTCGACACTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAACTCTACCTCAACGCCCTGAAAAGACCGCAAGGAATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCAAAAATCGGCTTTG
GCTTTGCCGAGGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCAGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACGCTGCACACCAACTCGGCCGCCGAAACCGT
GATTCGCTTAGCCAATATGGGGGTCGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTATATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q6PKK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

73.665

100

0.737
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

51.81

93.416

0.484
  pilB Acinetobacter baylyi ADP1

50.677

91.993

0.466
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.417

100

0.402
  pilF Thermus thermophilus HB27

40.296

96.263

0.388
  pilB Haemophilus influenzae 86-028NP

39.089

93.772

0.367

Multiple sequence alignment