Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   Achr_RS03355 Genome accession   NZ_CP010415
Coordinates   725572..727689 (+) Length   705 a.a.
NCBI ID   WP_167571668.1    Uniprot ID   -
Organism   Azotobacter chroococcum NCIMB 8003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 720572..732689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS03335 (Achr_6600) comM 722656..723720 (+) 1065 WP_039801858.1 pilus assembly protein PilM Machinery gene
  Achr_RS03340 (Achr_6610) - 723721..724377 (+) 657 WP_039801860.1 PilN domain-containing protein -
  Achr_RS03345 (Achr_6620) pilO 724374..724991 (+) 618 WP_039801862.1 type 4a pilus biogenesis protein PilO -
  Achr_RS03350 (Achr_6630) pilP 724994..725518 (+) 525 WP_039801864.1 type 4a pilus biogenesis lipoprotein PilP -
  Achr_RS03355 (Achr_6640) pilQ 725572..727689 (+) 2118 WP_167571668.1 type IV pilus secretin PilQ family protein Machinery gene
  Achr_RS03360 (Achr_6650) aroK 727694..728212 (+) 519 WP_082045358.1 shikimate kinase AroK -
  Achr_RS03365 (Achr_6660) aroB 728264..729367 (+) 1104 WP_039801868.1 3-dehydroquinate synthase -
  Achr_RS03370 (Achr_6670) - 729375..731021 (+) 1647 WP_039801870.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75906.14 Da        Isoelectric Point: 4.5580

>NTDB_id=136974 Achr_RS03355 WP_167571668.1 725572..727689(+) (pilQ) [Azotobacter chroococcum NCIMB 8003]
MNSTLSRLGISVLAALLAPELLAADLKALDVAALPGDRVELKLSFDEPVPAPRGYTIEQPARIALDLPGVANKLGTRYRE
LGVGNARSVTLVEAGGRTRMIVNLASLSPYSTRAEGNNLYVVIGESAAVPQQAGTAPSPVVTAAPVPLVKPYAPAGKAIQ
GIDFQRGEQGEGNVVIDLSSPGMNPDVQEEGGKIRLDFPGAQLPESLRARLDVKDFATPVQFVSASSEGGKASILIEPTG
MYDYLVYQTDNRLTVSVKPLTQENAAKRNADRFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGNITLRLQN
VPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQQQIAELAPLRRELVQVNYAKAADIAKLFQSVTSGEE
GEEQRGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSFVNDQWSLYGKDG
AQSIDDGQVFLPGTDQVGNFTLEDGVAPVPFVDMGVANSTSGLGIGFVSDHLILDLQLSAMEKTGNGEVISQPKVLTSDK
ETAKILTGEEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDQADFSKAIGGVPPINKNEVNAKVLIG
DGETIVIGGVFSNTQAKSVDKVPFLGDLPFIGRLFRRDVVADSKSELLVFLTPRILNNQAIAVSR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=136974 Achr_RS03355 WP_167571668.1 725572..727689(+) (pilQ) [Azotobacter chroococcum NCIMB 8003]
ATGAACAGCACCCTTTCGCGTCTTGGTATTTCAGTCCTGGCTGCCCTGCTGGCGCCGGAATTGTTGGCGGCCGACCTCAA
GGCTCTGGATGTGGCCGCCCTGCCTGGCGATCGGGTCGAGCTCAAGTTGTCCTTCGACGAGCCCGTGCCGGCGCCGCGCG
GCTATACCATCGAGCAGCCAGCCCGGATCGCCCTGGATCTGCCCGGTGTAGCCAACAAGCTGGGGACCCGGTATCGCGAA
CTGGGTGTCGGCAATGCCCGCAGCGTCACCCTGGTCGAGGCGGGCGGCCGCACCCGTATGATCGTCAACCTGGCGAGCCT
GTCGCCCTACAGCACGCGAGCCGAGGGCAACAACCTCTATGTGGTGATCGGCGAGTCGGCCGCCGTGCCCCAGCAGGCCG
GCACTGCACCGTCACCAGTCGTCACGGCGGCTCCGGTTCCGCTGGTCAAGCCCTATGCGCCGGCCGGCAAGGCCATCCAG
GGCATCGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAACGTCGTCATCGATCTGTCGAGTCCGGGGATGAATCCCGATGT
CCAGGAGGAGGGTGGCAAGATCCGCCTGGATTTCCCGGGCGCGCAGTTGCCGGAGTCCCTGCGGGCACGTCTGGATGTCA
AGGATTTCGCCACGCCCGTGCAGTTCGTGAGTGCTTCGAGTGAGGGTGGCAAGGCCAGCATCCTGATTGAGCCGACCGGC
ATGTACGACTATCTGGTCTACCAGACCGACAACCGCCTGACCGTCAGCGTCAAGCCGCTGACCCAGGAAAATGCCGCCAA
GCGCAATGCCGACCGCTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGATATCGATGTCCGCTCGGTCCTGC
AGCTGATCGCCGACTTCACCAACCTCAACCTGGTGGCCAGTGACACCGTGCAGGGCAATATCACCCTGCGCCTGCAGAAT
GTGCCCTGGGACCAGGCGCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTCCTGCTGGT
GGCGCCGGCCGACGAAATCGCGGCGCGCGAGCGTCAGGAGCTGGAGGCGCAGCAGCAGATCGCCGAGCTGGCCCCCCTGC
GCCGCGAGCTGGTCCAGGTGAACTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGCGGCGAAGAG
GGTGAGGAGCAGCGCGGTTCCATCACCGTCGACGAGCGGACCAACAACATCATCGCCTACCAGACCCAGGAGCGTCTAGA
CGAGCTGCGGCGGATCGTCGCCCAGCTGGACATTCCGGTGCGCCAGGTGATGATCGAGGCACGCATCGTCGAGGCCAATG
TCGATTACGACAAGAGCTTGGGGGTGCGCTGGGGCGGCAGCTTCGTGAACGACCAGTGGAGCCTGTATGGCAAGGATGGT
GCGCAATCCATCGATGACGGGCAGGTGTTTCTGCCCGGGACCGATCAGGTCGGCAACTTCACCCTGGAGGACGGTGTGGC
TCCCGTTCCCTTCGTCGACATGGGCGTGGCGAACAGCACATCGGGTCTCGGCATCGGCTTCGTTTCCGACCACCTGATTC
TCGACCTTCAGCTGTCGGCCATGGAAAAGACCGGCAATGGTGAGGTCATTTCCCAGCCCAAGGTTCTCACTTCGGACAAG
GAGACGGCGAAGATTCTGACCGGCGAGGAGATCCCCTACCAGGAAGCCAGCTCCAGCGGTGCGACCAGCACCTCCTTCAA
GGAAGCGGCGCTGTCGCTGGAGGTGACTCCGCAGATCACCCCCGACAACCGCATCATCATGGAGGTGAAGGTGACCAAGG
ACCAGGCGGACTTCTCCAAGGCGATAGGTGGAGTGCCGCCGATCAACAAGAACGAGGTCAACGCCAAGGTGCTGATCGGC
GACGGCGAAACCATCGTGATCGGCGGAGTCTTCTCCAATACCCAGGCCAAGTCCGTGGACAAGGTGCCCTTCCTCGGCGA
CCTGCCGTTCATCGGGCGCCTGTTCCGCCGCGACGTGGTCGCCGACAGCAAATCCGAGCTGCTTGTTTTCCTGACTCCGC
GTATTCTTAACAACCAGGCTATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(540-696)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.872

100

0.772
  comQ Acinetobacter baylyi ADP1

42.611

98.865

0.421
  pilQ Acinetobacter baumannii D1279779

40.366

100

0.407
  pilQ Acinetobacter baumannii strain A118

40.366

100

0.407
  pilQ Legionella pneumophila strain ERS1305867

38.257

96.028

0.367
  pilQ Legionella pneumophila strain Lp02

37.962

96.028

0.365

Multiple sequence alignment