Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   Achr_RS03335 Genome accession   NZ_CP010415
Coordinates   722656..723720 (+) Length   354 a.a.
NCBI ID   WP_039801858.1    Uniprot ID   -
Organism   Azotobacter chroococcum NCIMB 8003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717656..728720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Achr_RS03325 (Achr_6580) - 718682..719950 (+) 1269 WP_039801854.1 malic enzyme-like NAD(P)-binding protein -
  Achr_RS03330 (Achr_6590) - 720010..722475 (-) 2466 WP_193388183.1 penicillin-binding protein 1A -
  Achr_RS03335 (Achr_6600) comM 722656..723720 (+) 1065 WP_039801858.1 pilus assembly protein PilM Machinery gene
  Achr_RS03340 (Achr_6610) - 723721..724377 (+) 657 WP_039801860.1 PilN domain-containing protein -
  Achr_RS03345 (Achr_6620) pilO 724374..724991 (+) 618 WP_039801862.1 type 4a pilus biogenesis protein PilO -
  Achr_RS03350 (Achr_6630) pilP 724994..725518 (+) 525 WP_039801864.1 type 4a pilus biogenesis lipoprotein PilP -
  Achr_RS03355 (Achr_6640) pilQ 725572..727689 (+) 2118 WP_167571668.1 type IV pilus secretin PilQ family protein Machinery gene
  Achr_RS03360 (Achr_6650) aroK 727694..728212 (+) 519 WP_082045358.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37779.54 Da        Isoelectric Point: 4.7373

>NTDB_id=136973 Achr_RS03335 WP_039801858.1 722656..723720(+) (comM) [Azotobacter chroococcum NCIMB 8003]
MLGLFNKKAGSLLGIDISSTTVKLIELSRSGNRYRVEAYAVEPLPANAVVEKNIVDLEAVGQTLSKVLAKAKTGTKNAAV
AVAGSVVITKTVEMDAGLSEDELETQLKLEADQYIPYPLEEVAIDFEIQGPLPRSPGRAEVLLAACRKENVEAREAVLAL
AGLTAKVVDVEAYAMERACGLLVDQLGGDPAGLIVAVVDIGATMMTLSVMHHGRTLYTREQLFGGKQLTDEIQRRYGLSM
AEAGLAKKKGELPADYDSEVLQPFKDAVVQQVSRSLQFFFAAGQYNQVDYILLAGGTSSLPGLDQLIQQKTGTRTLVANP
FAEMALSPKVNASALVSDAPAMMVACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=136973 Achr_RS03335 WP_039801858.1 722656..723720(+) (comM) [Azotobacter chroococcum NCIMB 8003]
GTGCTAGGGCTCTTCAACAAGAAAGCGGGTTCATTACTGGGGATCGATATTAGTTCGACCACGGTCAAGCTTATCGAATT
GAGTCGTTCAGGTAACCGTTACCGGGTAGAGGCCTACGCTGTCGAGCCGCTTCCTGCCAATGCCGTCGTCGAGAAGAACA
TTGTCGATCTCGAGGCTGTCGGCCAGACCTTGAGCAAGGTGCTGGCCAAGGCCAAGACCGGAACCAAGAACGCGGCCGTG
GCCGTGGCGGGTTCCGTGGTGATTACCAAGACCGTCGAGATGGATGCCGGGCTTTCCGAGGATGAACTGGAAACCCAGTT
GAAGCTCGAGGCCGACCAATACATTCCCTATCCCCTCGAGGAAGTGGCGATCGACTTCGAGATCCAGGGGCCGCTGCCAC
GCAGCCCCGGACGCGCCGAAGTGCTGCTCGCCGCCTGCCGCAAGGAGAACGTCGAGGCCCGCGAGGCGGTGCTGGCACTG
GCCGGCCTGACGGCCAAGGTCGTCGATGTCGAGGCCTATGCCATGGAGCGCGCCTGCGGCCTGCTGGTCGACCAGTTGGG
CGGCGATCCGGCGGGGTTGATCGTGGCCGTCGTCGATATCGGCGCGACCATGATGACGCTCAGCGTAATGCATCACGGTC
GCACCCTCTATACCCGCGAGCAACTGTTCGGCGGCAAGCAGCTCACCGACGAAATCCAGCGCCGCTATGGCCTGTCCATG
GCCGAAGCCGGTCTGGCCAAGAAGAAGGGCGAACTTCCAGCAGACTATGACAGCGAGGTTTTGCAGCCGTTCAAGGACGC
TGTCGTACAGCAGGTTTCCCGTTCGCTGCAGTTCTTTTTCGCGGCGGGCCAGTACAACCAGGTCGACTACATCCTGCTGG
CGGGAGGCACCTCTTCCCTGCCGGGGCTTGATCAACTGATCCAGCAGAAGACCGGGACCCGGACGCTGGTGGCCAACCCG
TTTGCCGAGATGGCGTTGAGCCCCAAGGTGAATGCCTCGGCGCTGGTCAGCGATGCTCCAGCCATGATGGTTGCCTGCGG
TCTGGCGATGAGGAGTTTCGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.211

100

0.554
  pilM Acinetobacter baumannii D1279779

54.93

100

0.551
  comM Acinetobacter baylyi ADP1

52.958

100

0.531
  pilM Legionella pneumophila strain ERS1305867

44.633

100

0.446

Multiple sequence alignment