Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO356_RS24820 Genome accession   NZ_CP012831
Coordinates   5606515..5607933 (+) Length   472 a.a.
NCBI ID   WP_060742024.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5601515..5612933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS24805 (AO356_24780) gspL 5602239..5603420 (+) 1182 WP_060742021.1 type II secretion system protein GspL -
  AO356_RS24810 (AO356_24785) gspM 5603413..5603988 (+) 576 WP_060742022.1 type II secretion system protein GspM -
  AO356_RS24815 (AO356_24790) gspD 5603985..5606345 (+) 2361 WP_060742023.1 type II secretion system secretin GspD -
  AO356_RS24820 (AO356_24795) pilB 5606515..5607933 (+) 1419 WP_060742024.1 type II secretion system ATPase GspE Machinery gene
  AO356_RS24825 (AO356_24800) gspF 5607933..5609144 (+) 1212 WP_060742025.1 type II secretion system inner membrane protein GspF -
  AO356_RS24830 (AO356_24805) - 5609654..5610823 (+) 1170 WP_060742026.1 substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 51666.16 Da        Isoelectric Point: 5.4842

>NTDB_id=136780 AO356_RS24820 WP_060742024.1 5606515..5607933(+) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
MNTLPYAWAKAQRLVLRQSEEGAVLMVCPSTPGWSISEVRRQFGDVRLERVRDEELDGLLNSAYADTGSAAAVVGAAENE
VDLDRLMQDIPEITDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPYESHSVVRYRVDGTLRDVVSPRKALHGALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLQLETLGMDPDVLDKLDHLIRQPHG
IVLVTGPTGSGKTTSLYAALARLDASVHNILTVEDPVEYDLPGISQIQVNAKIDMTFALALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAINRLIDMGVEPFLLASSMLGVLAQRLVRRLCPQCKQPDPATPGTWRPVGCPS
CNQTGYSGRTGIHELFCIDDDIRTLIHQGAGEQALRAAARQAGMFSMREDGERWVRSGTTAPEEILRVTRDA

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=136780 AO356_RS24820 WP_060742024.1 5606515..5607933(+) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
ATGAACACCCTCCCATACGCCTGGGCCAAGGCCCAGCGCCTGGTGTTGCGCCAGTCCGAAGAGGGGGCGGTGCTGATGGT
GTGCCCGTCCACGCCGGGCTGGTCCATCAGTGAAGTGCGCCGTCAGTTCGGCGACGTGCGGCTGGAGCGGGTGCGCGACG
AGGAGCTCGACGGGCTGCTCAACAGTGCCTATGCCGACACCGGCAGTGCCGCCGCAGTGGTGGGCGCGGCGGAAAACGAA
GTCGACCTCGACCGGCTGATGCAGGACATCCCCGAGATCACCGACCTGCTGGACACCCAGGACGGCGCGCCGGTGATTCG
CATGATCAATGCCTTGCTGACCCAGGCCGCACGGGATGAGGCCAGCGACATCCACATCGAACCCTACGAGAGCCATTCAG
TGGTGCGCTATCGCGTCGACGGCACCCTGCGCGATGTGGTTTCGCCGCGCAAGGCCCTGCACGGTGCGCTGGTGTCGCGG
ATCAAGATCATGGCCCAGCTCGACATCGCCGAAAAACGCTTGCCCCAGGACGGCCGCATCGCTTTGCGCGTCGCCGGTCG
ACCGATCGATATCCGCGTCTCGACGGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGGTTGCTGGACAAACAGGCCG
GCCGCCTGCAACTGGAAACGTTGGGGATGGATCCGGACGTACTGGACAAGCTCGATCACCTGATCCGCCAGCCCCACGGC
ATCGTCCTGGTCACCGGCCCCACGGGCAGCGGCAAGACCACCAGCCTCTACGCCGCCCTGGCCCGGCTGGACGCCAGTGT
CCACAACATCCTCACGGTGGAGGACCCGGTGGAGTACGACCTGCCGGGCATCAGCCAGATCCAGGTCAACGCCAAGATCG
ACATGACCTTTGCCCTGGCGTTGCGGGCGATCCTGCGCCAGGACCCGGACATCATCATGATCGGCGAAATCCGCGACCTG
GAAACCGCACAAATCGCCGTGCAGGCGTCCCTCACCGGTCACCTGGTGCTGGCGACGCTGCACACCAACGACGCGGTGTC
GGCCATCAACCGCCTGATCGACATGGGGGTCGAGCCGTTCCTGCTGGCCTCGTCGATGCTCGGCGTGCTGGCCCAGCGAC
TGGTGCGCAGGTTGTGCCCGCAGTGCAAGCAACCAGATCCCGCCACGCCCGGCACCTGGCGCCCGGTAGGTTGCCCGAGC
TGTAACCAGACCGGCTACAGCGGCCGCACCGGGATCCATGAACTGTTCTGCATCGACGATGACATCCGCACGCTGATCCA
CCAGGGGGCAGGGGAGCAAGCACTGCGCGCCGCCGCCCGCCAGGCCGGCATGTTCAGCATGCGCGAGGATGGCGAGCGCT
GGGTGCGCAGTGGCACCACGGCCCCTGAAGAAATCCTGCGCGTGACACGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.22

100

0.432
  pilB Acinetobacter baumannii D1279779

41.463

100

0.432
  pilF Neisseria gonorrhoeae MS11

44.843

94.492

0.424
  pilB Vibrio campbellii strain DS40M4

48.608

83.686

0.407
  pilB Vibrio parahaemolyticus RIMD 2210633

48.223

83.475

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.436

82.627

0.392
  pilB Legionella pneumophila strain ERS1305867

44.878

86.864

0.39
  pilB Acinetobacter baylyi ADP1

46.907

82.203

0.386
  pilF Thermus thermophilus HB27

48.404

79.661

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

42.08

89.619

0.377
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.627

90.89

0.36

Multiple sequence alignment