Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IX92_RS12930 Genome accession   NZ_CP009617
Coordinates   2814567..2816213 (+) Length   548 a.a.
NCBI ID   WP_043009226.1    Uniprot ID   A0AAN0VY95
Organism   Vibrio coralliilyticus strain RE98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2809567..2821213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX92_RS12900 (IX92_12900) - 2809832..2810584 (-) 753 WP_006958128.1 thioredoxin fold domain-containing protein -
  IX92_RS12905 (IX92_12905) xerD 2810624..2811520 (-) 897 WP_031343348.1 site-specific tyrosine recombinase XerD -
  IX92_RS12910 (IX92_12910) fldB 2811684..2812205 (+) 522 WP_038163561.1 flavodoxin FldB -
  IX92_RS12915 (IX92_12915) ampD 2812332..2812877 (-) 546 WP_006958131.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IX92_RS12920 (IX92_12920) nadC 2813009..2813896 (+) 888 WP_021458291.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IX92_RS12925 (IX92_12925) - 2814124..2814564 (+) 441 WP_043009222.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IX92_RS12930 (IX92_12930) pilB 2814567..2816213 (+) 1647 WP_043009226.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IX92_RS12935 (IX92_12935) - 2816222..2817457 (+) 1236 WP_043009229.1 type II secretion system F family protein -
  IX92_RS12940 (IX92_12940) pilD 2817507..2818376 (+) 870 WP_043009231.1 A24 family peptidase Machinery gene
  IX92_RS12945 (IX92_12945) coaE 2818378..2818986 (+) 609 WP_043009232.1 dephospho-CoA kinase -
  IX92_RS12950 (IX92_12950) zapD 2819020..2819760 (+) 741 WP_043009234.1 cell division protein ZapD -
  IX92_RS12955 (IX92_12955) yacG 2819812..2820006 (+) 195 WP_006958142.1 DNA gyrase inhibitor YacG -
  IX92_RS12960 (IX92_12960) rplS 2820252..2820605 (-) 354 WP_006958143.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 60686.13 Da        Isoelectric Point: 4.8031

>NTDB_id=130781 IX92_RS12930 WP_043009226.1 2814567..2816213(+) (pilB) [Vibrio coralliilyticus strain RE98]
MSELPFLLKKANKISEEQALQAISATETRQIEAYSALLEISDLTSKQLTAAISELYALPILSLDDFDYRTCCEQLGIGDL
IRSHTALPLEVERQHLTLALADPSISEIEQEFRFATGLAIKLVLAELPTLARAINNLYGAQSASDSLGYKEVSALDLEQL
VEAEEHELIENEELNQDSSPISRYIQQILLDAVRKRASDIHFEPYDACFRVRFRCDGLLVEVQRPPQHLCQRLTARLKIL
AKLDIAERRLPQDGRITLHVTPTDSIDIRVSTLPTIWGEKVVLRLLDNRMTSFDLDSLGYNRVQSESYRQALTKPQGLIL
ITGPTGSGKTLSLYSGLNIINQNEVNIATAEDPVEIHLTGINQVQIQPQIGFNFAQALRAFLRQDPDVVMVGEIRDRETA
EIATKAAQTGHLVLSTLHTNSAAEAILRLQNMGIETFNLAASLSLIIAQRLVRCLCPQCKRPTKNQTGHYEASSEGCQDC
NQGYSGRTGIYEVLTVTPEIAQAVNEGSTALQIEDLAIQQGMQTLQHAGLEKLAQGITSQAELQRVLI

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=130781 IX92_RS12930 WP_043009226.1 2814567..2816213(+) (pilB) [Vibrio coralliilyticus strain RE98]
ATGAGTGAACTGCCCTTCTTGCTGAAAAAAGCCAATAAAATTAGTGAAGAACAAGCCCTGCAAGCAATATCAGCTACAGA
AACCCGACAAATTGAGGCGTATTCTGCTCTGCTAGAGATTAGTGACCTGACATCCAAACAGCTGACAGCAGCTATCAGTG
AACTCTATGCTCTCCCTATCTTGTCGTTAGATGACTTCGATTATCGAACTTGTTGCGAGCAGCTCGGCATTGGCGACTTA
ATTCGCAGCCACACGGCACTGCCACTAGAAGTAGAGCGACAACATCTTACTCTAGCGCTCGCCGATCCTTCCATTTCCGA
GATAGAACAAGAATTTCGTTTTGCAACTGGCCTAGCAATCAAACTCGTATTGGCCGAGTTACCGACTTTAGCCCGTGCGA
TAAACAACCTATATGGAGCTCAAAGCGCCAGTGACTCTTTAGGCTACAAAGAGGTTAGCGCCCTAGACCTTGAGCAGTTA
GTCGAAGCTGAAGAACATGAATTAATAGAGAATGAGGAGCTCAATCAAGATAGCTCGCCAATCAGTCGCTATATTCAGCA
GATACTGCTGGATGCCGTGCGCAAGCGAGCCTCTGATATCCATTTTGAACCTTATGACGCATGCTTTCGCGTCCGCTTTC
GTTGTGACGGCTTGCTTGTTGAAGTTCAGCGACCGCCTCAGCACCTATGCCAAAGGTTAACCGCTAGACTCAAGATCCTC
GCTAAACTCGACATCGCCGAGCGCAGACTCCCGCAAGATGGTCGCATCACTCTCCATGTGACTCCAACCGATTCCATCGA
CATACGTGTATCGACTCTACCTACCATATGGGGCGAAAAAGTTGTCCTTAGATTGCTCGACAACAGAATGACCAGCTTTG
ACTTAGACAGCCTTGGCTACAATCGCGTCCAAAGCGAAAGCTATCGCCAAGCACTGACTAAGCCTCAGGGACTCATTCTA
ATCACAGGCCCAACGGGAAGCGGTAAGACTCTGTCTTTATACAGTGGGCTTAACATCATTAATCAAAATGAAGTCAACAT
CGCGACAGCCGAAGACCCGGTAGAAATTCACCTGACAGGAATCAATCAGGTGCAAATTCAGCCTCAAATCGGCTTCAATT
TCGCTCAGGCACTCAGAGCGTTTCTACGTCAGGACCCCGATGTGGTTATGGTGGGGGAAATTCGAGATCGAGAAACCGCC
GAGATAGCTACCAAAGCGGCTCAAACAGGCCACTTAGTCCTTTCAACACTCCATACTAACTCAGCAGCAGAAGCCATTCT
TCGCCTCCAAAACATGGGAATAGAAACGTTCAACCTTGCCGCTTCTCTTAGCCTCATCATTGCCCAGCGCTTGGTCAGGT
GCCTATGCCCTCAATGCAAGCGCCCTACAAAAAACCAGACGGGCCACTACGAGGCTTCTTCTGAAGGTTGTCAGGACTGC
AACCAAGGCTACTCAGGACGAACTGGTATTTATGAGGTACTCACCGTAACACCTGAAATTGCACAAGCCGTCAATGAAGG
CTCCACTGCACTCCAAATTGAGGACTTAGCGATACAACAAGGCATGCAAACATTACAGCACGCGGGCTTAGAAAAATTGG
CCCAAGGTATCACTAGCCAGGCAGAACTGCAGCGAGTGCTAATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.172

100

0.573
  pilB Vibrio parahaemolyticus RIMD 2210633

56.014

100

0.569
  pilB Vibrio campbellii strain DS40M4

55.376

100

0.564
  pilB Legionella pneumophila strain ERS1305867

47.419

95.438

0.453
  pilB Acinetobacter baumannii D1279779

44.732

98.723

0.442
  pilB Acinetobacter baylyi ADP1

45.627

95.985

0.438
  pilF Neisseria gonorrhoeae MS11

42.802

95.073

0.407
  pilB Haemophilus influenzae Rd KW20

43.59

85.401

0.372
  pilB Haemophilus influenzae 86-028NP

42.949

85.401

0.367
  pilB Glaesserella parasuis strain SC1401

40.909

88.321

0.361

Multiple sequence alignment