Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JM49_RS27535 Genome accession   NZ_CP009290
Coordinates   6156263..6158326 (-) Length   687 a.a.
NCBI ID   WP_038582720.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain JD37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6151263..6163326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM49_RS27520 (JM49_28080) - 6152891..6154480 (-) 1590 WP_038582717.1 AAA family ATPase -
  JM49_RS27525 (JM49_28085) aroB 6154491..6155591 (-) 1101 WP_016704447.1 3-dehydroquinate synthase -
  JM49_RS27530 (JM49_28090) aroK 6155740..6156258 (-) 519 WP_009041824.1 shikimate kinase AroK -
  JM49_RS27535 (JM49_28095) pilQ 6156263..6158326 (-) 2064 WP_038582720.1 type IV pilus secretin PilQ Machinery gene
  JM49_RS27540 (JM49_28100) - 6158340..6158867 (-) 528 WP_038582723.1 pilus assembly protein PilP -
  JM49_RS27545 (JM49_28105) - 6158864..6159481 (-) 618 WP_038582725.1 type 4a pilus biogenesis protein PilO -
  JM49_RS27550 (JM49_28110) - 6159478..6160044 (-) 567 WP_038582728.1 PilN domain-containing protein -
  JM49_RS27555 (JM49_28115) pilM 6160044..6161096 (-) 1053 WP_038582730.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 74416.50 Da        Isoelectric Point: 5.4337

>NTDB_id=128318 JM49_RS27535 WP_038582720.1 6156263..6158326(-) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain JD37]
MNRIFSAFGIALWMALLSPMAMAASLKALDVAALPGDRLELKLTFDGPVPEPRGYTTDQPARIALDLPGVVSQLTNKSRD
LGNGNARSVTVVESGQRTRVVVNLVALAPYSTRVAGNQLFVVIGQGATAAQSAPGQPAVASGRAKAAASAGRSIRSVDFQ
RGEQGEGNVLIDLSDSGMSPDIQERDGKLVLTFAKARLPEPLRVRLDVKDFATPVQFVSARAESGKAIVTIEPGGTFDYS
TYQTDNKLTVSVRPMTSGDLQKRNTERSAYNGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQ
ALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLEPLRRELLQVNYAKAAEIAKLFQSVTRAEDKSDER
GSITVDERTNNIIAYQTRERLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNQGNWNASGVSNGSST
IGTPGSTSSNSPFVDLGTTANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAAS
SGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLINDGETIVIGGVFSNTQSKV
VDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=128318 JM49_RS27535 WP_038582720.1 6156263..6158326(-) (pilQ) [Pseudomonas chlororaphis subsp. aurantiaca strain JD37]
ATGAACAGGATCTTTTCAGCCTTCGGTATTGCGCTATGGATGGCGCTGCTTTCACCGATGGCCATGGCGGCCAGCCTCAA
GGCCCTGGATGTCGCGGCACTGCCGGGGGATCGCCTGGAGTTGAAACTCACCTTCGATGGGCCGGTGCCCGAGCCCCGGG
GTTACACCACCGACCAGCCGGCGCGGATCGCCCTCGATCTGCCGGGTGTCGTCAGCCAGCTGACGAACAAGAGCCGCGAT
CTGGGCAATGGCAATGCACGCAGCGTGACAGTAGTCGAATCGGGGCAGCGTACCCGGGTGGTGGTCAACCTGGTCGCGCT
GGCGCCCTACAGTACTCGAGTTGCCGGCAATCAGCTGTTTGTGGTGATCGGCCAGGGGGCGACGGCGGCGCAGAGTGCTC
CGGGGCAGCCGGCTGTCGCATCCGGGCGGGCCAAGGCCGCGGCTTCGGCGGGGCGGTCGATTCGCAGCGTGGATTTCCAG
CGCGGCGAGCAGGGTGAGGGCAACGTACTGATCGACCTCTCCGACTCGGGAATGAGCCCGGATATCCAGGAGCGCGACGG
CAAGCTCGTCCTTACCTTCGCCAAGGCCCGCCTGCCAGAGCCCCTGCGGGTCAGGCTGGACGTCAAGGACTTCGCCACCC
CCGTGCAATTCGTCAGCGCCCGGGCGGAGTCCGGCAAGGCCATCGTCACCATCGAGCCCGGCGGCACCTTCGATTACTCG
ACCTACCAGACGGACAACAAGCTCACGGTAAGCGTCAGGCCCATGACTTCGGGCGATCTGCAGAAACGCAACACCGAGCG
CTCGGCCTATAACGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGAGGTCCGCTCGGTGCTGCAACTGATCGCCGACT
TCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATTACCTTGCGCCTGCAAAATGTGCCGTGGGACCAG
GCTCTCGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTGGGCAATGTGTTGCTGGTGGCGCCAGCCGATGA
AATCGCCGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGATCTCGAGCCGCTGCGGCGTGAACTGCTGC
AGGTCAACTACGCCAAGGCCGCGGAGATCGCCAAGCTGTTCCAGTCGGTCACCCGCGCTGAGGACAAGTCTGACGAGCGT
GGTTCGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCGGGAGCGCCTGGACGAGCTGCGGCGGAT
TGTCAGCCAGCTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCGACTACGACAAAA
GCCTGGGCGTGCGCTGGGGTGGTTCGATCCAGAACCAGGGCAACTGGAATGCCTCGGGGGTCAGCAACGGCTCTTCCACC
ATAGGCACGCCAGGCAGTACCAGCAGCAACTCACCGTTCGTCGACCTGGGCACCACCGCCAATACCTCCGGCCTGGGTAT
CGCCTTCATCACCGACAACGTGTTGCTGGACCTTGAACTGACAGCCATGGAAAAGACCGGTAACGGGGAAATCGTCTCCC
AGCCCAAGGTGGTCACCTCCGACAAAGAGACGGCGAAGATTCTCAAGGGTACGGAAATCCCCTATCAGGAAGCCGCCTCC
AGCGGGGCGACTTCGGTGTCGTTCAAGGAGGCGTCGCTGTCCCTGGAGGTGACGCCGCAGATCACCCCGGATAACCGCAT
CATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTCCAGGACGTGCCGCCGATCAAGAAGAACG
AGGTCAACGCCAAGGTCCTGATCAACGACGGAGAAACCATCGTTATCGGTGGGGTTTTCTCCAATACGCAAAGCAAGGTT
GTAGATAAGGTGCCATTTCTAGGCGATGTGCCGTATCTTGGCCGCCTTTTCAGGCGTGACGTGGTGTCGGAGAAAAAATC
CGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-122)

Secretin and TonB N terminus short domain (STN)

(298-344)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-437)

Bacterial type II and III secretion system protein (Secretin)

(522-678)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.629

100

0.735
  comQ Acinetobacter baylyi ADP1

40.028

100

0.424
  pilQ Acinetobacter baumannii strain A118

37.652

100

0.406
  pilQ Acinetobacter baumannii D1279779

37.652

100

0.406
  pilQ Legionella pneumophila strain Lp02

38.039

97.962

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.039

97.962

0.373

Multiple sequence alignment