Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JM49_RS27555 Genome accession   NZ_CP009290
Coordinates   6160044..6161096 (-) Length   350 a.a.
NCBI ID   WP_038582730.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis subsp. aurantiaca strain JD37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6155044..6166096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM49_RS27530 (JM49_28090) aroK 6155740..6156258 (-) 519 WP_009041824.1 shikimate kinase AroK -
  JM49_RS27535 (JM49_28095) pilQ 6156263..6158326 (-) 2064 WP_038582720.1 type IV pilus secretin PilQ Machinery gene
  JM49_RS27540 (JM49_28100) - 6158340..6158867 (-) 528 WP_038582723.1 pilus assembly protein PilP -
  JM49_RS27545 (JM49_28105) - 6158864..6159481 (-) 618 WP_038582725.1 type 4a pilus biogenesis protein PilO -
  JM49_RS27550 (JM49_28110) - 6159478..6160044 (-) 567 WP_038582728.1 PilN domain-containing protein -
  JM49_RS27555 (JM49_28115) pilM 6160044..6161096 (-) 1053 WP_038582730.1 pilus assembly protein PilM Machinery gene
  JM49_RS27560 (JM49_28120) - 6161313..6163748 (+) 2436 WP_164485365.1 penicillin-binding protein 1A -
  JM49_RS27565 (JM49_28125) - 6163876..6165144 (-) 1269 WP_007925851.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 37105.59 Da        Isoelectric Point: 5.1221

>NTDB_id=128319 JM49_RS27555 WP_038582730.1 6160044..6161096(-) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain JD37]
MLGLFSKKASSLLGIDISSTSVKLLELSRVGGRFRVEAYGVEPLPANAVVEKNIVEPEGVGHALSRLLARARPGAKHAAL
AMAGSAVISKVIEMEAGLCDDDRESQLKVDADQYIPYPLDEAAIDFDVLGYSARNPERVDVLLAACRKEHIEVREAALEL
AGLTARVVDLEVCALERSFALAAGASVARTVALVDIGATLTTLSVVHEGQIIYSREQLFGGHQLTEHIQRRYGLSKEEAG
LAKKQGGLPDDYTSEVLRPFQEAVVQQVSRSLQFFQASGPQRQVDSILLAGGSASVPGLAPLIEQRLGLPVLIANPFAGM
SIGGKVDAAALASEAPSLMIACGLALRSFD

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=128319 JM49_RS27555 WP_038582730.1 6160044..6161096(-) (pilM) [Pseudomonas chlororaphis subsp. aurantiaca strain JD37]
GTGCTAGGACTCTTCAGTAAAAAGGCGAGTTCCCTTCTGGGGATCGATATCAGCTCCACCTCGGTCAAGCTTCTTGAGTT
GAGCCGTGTCGGCGGCCGTTTTCGGGTCGAGGCCTATGGGGTGGAACCGTTGCCGGCCAACGCCGTGGTCGAAAAAAACA
TCGTCGAGCCCGAAGGGGTGGGGCATGCCTTGTCTCGCCTGCTGGCCAGGGCCCGGCCGGGAGCAAAGCACGCGGCGCTG
GCGATGGCCGGTTCGGCGGTGATCAGCAAGGTCATCGAGATGGAGGCCGGGCTTTGCGATGACGACCGGGAGAGCCAGCT
CAAGGTCGACGCCGACCAGTACATCCCTTATCCGCTGGATGAGGCCGCCATCGATTTCGACGTCCTCGGCTATTCGGCGC
GCAATCCCGAACGCGTCGATGTGCTGTTGGCGGCCTGCCGCAAGGAACACATCGAAGTTCGCGAGGCGGCGCTGGAGCTC
GCCGGGCTGACGGCGCGGGTGGTGGACCTGGAAGTCTGCGCGCTGGAGCGCTCGTTCGCCCTGGCGGCCGGGGCGTCGGT
TGCCCGGACTGTGGCGCTGGTCGATATCGGAGCCACCCTGACCACCCTGAGCGTTGTGCATGAAGGGCAAATCATCTATT
CCCGCGAGCAGTTGTTTGGTGGGCACCAACTGACCGAGCACATCCAGCGTCGTTACGGGCTGTCGAAAGAAGAGGCCGGC
CTGGCAAAAAAACAGGGCGGCCTGCCGGACGATTACACCAGTGAGGTGCTGCGGCCCTTTCAGGAGGCGGTCGTGCAGCA
GGTTTCGCGCTCGTTGCAGTTTTTCCAGGCCTCCGGCCCGCAGCGCCAGGTCGACTCCATCCTGTTGGCGGGCGGCAGCG
CCTCGGTTCCGGGACTTGCGCCGTTGATCGAGCAGCGCCTGGGCTTGCCCGTGCTGATCGCCAACCCCTTCGCTGGCATG
AGCATCGGCGGCAAGGTCGACGCCGCGGCCCTGGCCAGCGAGGCTCCATCCTTGATGATCGCCTGTGGGCTGGCCTTGCG
GAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.398

100

0.557
  comM Acinetobacter nosocomialis M2

55.114

100

0.554
  comM Acinetobacter baylyi ADP1

52.841

100

0.531
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.46

Multiple sequence alignment