Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JV59_RS01450 Genome accession   NZ_CP009264
Coordinates   272046..273692 (-) Length   548 a.a.
NCBI ID   WP_038509654.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain OCN014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 267046..278692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JV59_RS01420 (JV59_26570) rplS 267638..267991 (+) 354 WP_006958143.1 50S ribosomal protein L19 -
  JV59_RS01425 (JV59_26575) yacG 268253..268447 (-) 195 WP_019277521.1 DNA gyrase inhibitor YacG -
  JV59_RS01430 (JV59_01410) zapD 268499..269239 (-) 741 WP_038509645.1 cell division protein ZapD -
  JV59_RS01435 (JV59_01415) coaE 269273..269881 (-) 609 WP_038509649.1 dephospho-CoA kinase -
  JV59_RS01440 (JV59_01420) pilD 269883..270752 (-) 870 WP_006958139.1 prepilin peptidase Machinery gene
  JV59_RS01445 (JV59_01425) - 270802..272037 (-) 1236 WP_038509652.1 type II secretion system F family protein -
  JV59_RS01450 (JV59_01430) pilB 272046..273692 (-) 1647 WP_038509654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JV59_RS01455 (JV59_26580) - 273695..274135 (-) 441 WP_031343346.1 pilin -
  JV59_RS01460 (JV59_01440) nadC 274363..275250 (-) 888 WP_019277527.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JV59_RS01465 (JV59_01445) ampD 275382..275927 (+) 546 WP_038509658.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JV59_RS01470 (JV59_01450) fldB 276055..276576 (-) 522 WP_038514565.1 flavodoxin FldB -
  JV59_RS01475 (JV59_01455) xerD 276740..277636 (+) 897 WP_031343348.1 site-specific tyrosine recombinase XerD -
  JV59_RS01480 (JV59_01460) - 277676..278428 (+) 753 WP_006958128.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 60756.16 Da        Isoelectric Point: 4.8436

>NTDB_id=127848 JV59_RS01450 WP_038509654.1 272046..273692(-) (pilB) [Vibrio coralliilyticus strain OCN014]
MSELPFLLKKANKISEEQALQAISATETRQIEAYSALLEISDLTSKQLTAAISELYALPILSLDDFDYRTCCEQLGIGDL
IRSHTALPLEVERQHLTLALADPSISEIEQEFRFATGLAIKLVLAELPTLARAINNLYGAQSASDSLGYKEVSALDLEQL
VEAEEHELIDSEDLNHDSSPISRYIQQILLDAVRKQASDIHFEPHESWFRVRFRCDGLLVEVQRPPQHLCQRLTARLKIL
AKLDIAERRLPQDGRITLNVTQTDSIDIRVSTLPTIWGEKIVLRLLDNRMTSFDLSSLGYNHIQNESYRQALTKPQGLIL
ITGPTGSGKTLSLYSGLNIINQNEINIATAEDPVEIHLTGINQVQIQPQIGFDFAQALRAFLRQDPDVVMVGEIRDRETA
EIATKAAQTGHLVLSTLHTNSAAEAILRLQNMGIEAFNLAASLSLIIAQRLIRCLCPQCKRPTKVQTGQYEASSKGCQDC
NQGYSGRTGIYEVLTVTPEIAQAINDDATAHQIEDLAIQQGMQTLPHSGLEKLSQGITSQAELQRVLI

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=127848 JV59_RS01450 WP_038509654.1 272046..273692(-) (pilB) [Vibrio coralliilyticus strain OCN014]
ATGAGTGAACTGCCCTTCTTGCTGAAAAAAGCCAATAAAATTAGTGAAGAACAAGCCCTGCAAGCAATATCAGCTACAGA
AACCCGACAAATTGAGGCGTATTCTGCTCTGCTAGAGATTAGTGACCTGACATCCAAACAGCTGACAGCAGCTATCAGTG
AACTCTATGCTCTCCCTATCTTGTCGTTAGATGACTTCGATTATCGAACTTGTTGCGAGCAGCTCGGCATTGGCGACTTA
ATTCGCAGCCACACGGCACTGCCACTAGAAGTAGAGCGACAACATCTTACTCTAGCGCTCGCCGATCCTTCCATTTCCGA
GATAGAACAAGAATTTCGTTTTGCAACTGGCCTAGCAATCAAACTCGTATTGGCCGAGTTACCGACTTTAGCCCGTGCGA
TAAACAACCTATATGGAGCTCAAAGCGCCAGTGACTCTTTAGGCTACAAAGAGGTTAGCGCCCTAGACCTTGAGCAGTTA
GTCGAAGCTGAAGAACATGAATTAATAGACAGTGAGGATCTCAATCACGATAGCTCGCCAATCAGTCGCTATATCCAGCA
GATACTGCTGGATGCCGTACGTAAGCAAGCTTCTGATATCCATTTTGAACCTCATGAATCATGGTTTCGCGTCAGGTTTC
GTTGTGATGGCTTGCTTGTTGAAGTGCAGCGACCTCCTCAGCACTTATGCCAAAGGTTAACCGCCAGACTCAAAATCCTC
GCTAAGCTGGACATCGCCGAGCGCAGACTCCCGCAAGATGGCCGTATCACTCTCAATGTGACTCAAACTGATTCTATCGA
CATACGTGTATCGACTCTGCCCACCATATGGGGTGAAAAAATTGTCCTTAGATTGCTCGACAACAGAATGACCAGCTTTG
ACTTAAGCAGCCTTGGCTACAATCACATTCAAAACGAAAGCTATCGTCAGGCGCTGACCAAGCCTCAGGGACTCATTCTA
ATCACTGGCCCCACGGGAAGCGGTAAGACTCTGTCTTTATACAGTGGACTTAACATCATCAATCAGAATGAGATCAACAT
CGCGACGGCAGAAGATCCCGTAGAGATTCACCTAACTGGAATCAATCAGGTGCAAATTCAACCTCAAATCGGCTTTGATT
TCGCTCAGGCACTCAGAGCCTTTCTACGTCAGGATCCCGATGTGGTTATGGTGGGTGAAATTCGCGATCGAGAAACTGCC
GAGATAGCCACCAAAGCGGCTCAAACAGGCCATTTAGTCCTTTCAACACTCCATACCAACTCAGCAGCAGAAGCTATTCT
TCGCCTCCAGAATATGGGAATAGAAGCTTTCAACCTTGCTGCTTCTCTTAGCCTCATCATTGCTCAGCGTTTGATCAGGT
GCCTATGCCCTCAATGCAAGCGCCCTACAAAAGTCCAGACAGGTCAATATGAGGCTTCTTCTAAAGGTTGTCAGGACTGC
AACCAAGGCTACTCAGGACGAACTGGTATTTATGAAGTACTCACAGTGACACCTGAAATTGCGCAAGCTATTAACGACGA
CGCCACAGCTCACCAGATTGAGGACTTAGCGATACAACAAGGCATGCAAACATTACCGCATTCGGGCTTAGAAAAGCTGT
CCCAAGGTATCACTAGCCAGGCAGAACTGCAGCGAGTGCTAATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.887

100

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

56.553

100

0.575
  pilB Vibrio campbellii strain DS40M4

56.093

100

0.571
  pilB Legionella pneumophila strain ERS1305867

46.845

95.438

0.447
  pilB Acinetobacter baylyi ADP1

46.198

95.985

0.443
  pilB Acinetobacter baumannii D1279779

44.547

98.723

0.44
  pilF Neisseria gonorrhoeae MS11

41.843

95.073

0.398
  pilB Haemophilus influenzae Rd KW20

44.444

85.401

0.38
  pilB Haemophilus influenzae 86-028NP

43.803

85.401

0.374
  pilB Glaesserella parasuis strain SC1401

41.116

88.321

0.363

Multiple sequence alignment