Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NJ8700_RS06725 Genome accession   NZ_CP009230
Coordinates   1470413..1471822 (+) Length   469 a.a.
NCBI ID   WP_005701263.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus NJ8700     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1465413..1476822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NJ8700_RS06705 (NJ8700_06865) pnp 1465918..1468047 (-) 2130 WP_005701259.1 polyribonucleotide nucleotidyltransferase -
  NJ8700_RS06710 (NJ8700_06870) nanQ 1468317..1468787 (+) 471 WP_005701260.1 N-acetylneuraminate anomerase -
  NJ8700_RS06715 (NJ8700_06875) ampD 1469260..1469814 (-) 555 WP_012771713.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NJ8700_RS06720 (NJ8700_06880) pilA 1469939..1470388 (+) 450 WP_005701262.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NJ8700_RS06725 (NJ8700_06885) pilB 1470413..1471822 (+) 1410 WP_005701263.1 GspE/PulE family protein Machinery gene
  NJ8700_RS06730 (NJ8700_06890) pilC 1471815..1473053 (+) 1239 WP_005701265.1 type II secretion system F family protein Machinery gene
  NJ8700_RS06735 (NJ8700_06895) - 1473050..1473742 (+) 693 WP_005701266.1 A24 family peptidase -
  NJ8700_RS06740 (NJ8700_06900) coaE 1473795..1474415 (+) 621 WP_005701267.1 dephospho-CoA kinase -
  NJ8700_RS06745 (NJ8700_06905) yacG 1474408..1474620 (+) 213 WP_005701268.1 DNA gyrase inhibitor YacG -
  NJ8700_RS06750 (NJ8700_06910) - 1474620..1474886 (+) 267 WP_005701270.1 GNAT family N-acetyltransferase -
  NJ8700_RS06755 (NJ8700_06915) - 1475253..1476626 (+) 1374 WP_005701272.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52839.28 Da        Isoelectric Point: 6.0101

>NTDB_id=127406 NJ8700_RS06725 WP_005701263.1 1470413..1471822(+) (pilB) [Aggregatibacter aphrophilus NJ8700]
MQHSMEQPYSICSQQGDIFMITPELWQQNQQQQTVLLRYLALPLKEEPQKLWLGLDSLTNLAACEAFSFLTGKNIEPVLI
ESAVLKTALQDLAPHQEKLDENQPLFYSVTPQEQQEQSSDEPTIQLLNQIFENATTKKASDIHLEPQTDFLQVRFRIDGV
LQVQNTIAQTLANRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLATHFGEKAVLRLQQNRPVQLAFSEL
GMTEQQQQTFRHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIQLPGIIQSQVNPQIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAASAISRLQQLGIQQHEIDSSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRIGVYQFLQPNVSQISGYQLDFQNLYASALEKVKSQVTDLAEVQRVLGQPNA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=127406 NJ8700_RS06725 WP_005701263.1 1470413..1471822(+) (pilB) [Aggregatibacter aphrophilus NJ8700]
ATGCAACATTCGATGGAACAACCTTATTCTATCTGTAGCCAACAGGGCGACATATTTATGATTACGCCCGAGTTATGGCA
ACAAAACCAACAGCAACAAACCGTCCTTTTGCGTTATTTGGCGCTGCCATTAAAAGAAGAACCACAAAAATTATGGCTGG
GATTGGATTCCTTAACCAACCTTGCCGCTTGTGAAGCTTTTTCCTTTTTAACGGGGAAAAATATCGAGCCGGTACTCATT
GAAAGTGCCGTGTTAAAAACGGCACTGCAAGATTTGGCGCCACATCAAGAAAAACTCGATGAAAATCAACCGCTCTTTTA
TTCCGTCACACCGCAGGAGCAACAAGAACAATCCTCTGATGAGCCGACAATTCAATTGCTTAACCAAATTTTCGAAAACG
CTACAACAAAAAAAGCCTCCGATATTCATTTAGAACCGCAAACGGATTTTTTACAAGTCCGTTTTCGTATTGACGGCGTA
TTACAAGTGCAAAACACCATTGCGCAGACACTCGCCAATCGACTGATTTCCCGTCTGAAATTACTGGCCAAATTAGACAT
CAGCGAAACCCGCCTGCCACAAGACGGACGGTTTCAGTTTAAAACGACATTTTCCGATATTTTGGATTTTCGCTTATCCA
CACTCGCGACGCATTTTGGCGAAAAAGCCGTATTGCGTTTACAACAGAACCGCCCCGTACAACTGGCCTTTAGCGAACTG
GGTATGACGGAACAACAGCAACAAACATTTCGTCATGCGCTCAGCCAACCGCAAGGGCTGATTTTAGTCACCGGTCCCAC
GGGAAGTGGCAAAAGTATTTCTTTATATACGGCATTACAATGGCTCAATACCCCCGACAAGCATATTATGACGGCAGAAG
ATCCCATTGAAATTCAATTACCGGGCATTATCCAAAGCCAAGTGAATCCTCAAATCGGCTTGGATTTCAGTCGGTTGTTA
CGCACTTTTTTGCGTCAAGATCCCGACATTATTATGTTGGGTGAAATTCGTGACGAAGAAAGTGCCGCCATGGCTTTACG
GGCGGCGCAAACCGGGCATTTGGTATTATCGACGTTACATACCAATGACGCCGCCTCAGCCATCTCACGCCTACAACAGC
TTGGCATTCAACAACACGAAATCGACAGTAGTTTATTATTGGTTATCGCCCAACGCTTAGTGCGTAAACGATGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGTGATTGTCATCAAGGCTACAAAGGTCGAATCGGTGTTTATCAATTCCTTCAGCC
TAATGTGTCGCAAATCTCAGGTTATCAATTGGATTTTCAGAATTTATATGCCAGTGCCTTAGAAAAAGTAAAGTCGCAAG
TGACGGATTTAGCAGAAGTACAACGGGTATTGGGGCAACCGAATGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

73.638

97.868

0.721
  pilB Haemophilus influenzae Rd KW20

72.985

97.868

0.714
  pilB Glaesserella parasuis strain SC1401

58.874

98.507

0.58
  pilB Vibrio campbellii strain DS40M4

41.962

100

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

41.962

100

0.429
  pilB Vibrio cholerae strain A1552

41.458

100

0.424
  pilB Acinetobacter baumannii D1279779

40.825

100

0.422
  pilB Legionella pneumophila strain ERS1305867

38.645

100

0.414
  pilB Acinetobacter baylyi ADP1

38.6

100

0.412
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

89.552

0.362

Multiple sequence alignment