Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JL26_RS08185 Genome accession   NZ_CP009158
Coordinates   1635502..1636887 (+) Length   461 a.a.
NCBI ID   WP_038514696.1    Uniprot ID   -
Organism   Glaesserella parasuis strain SH03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1630502..1641887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JL26_RS08160 (JL26_08400) gpt 1630874..1631353 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  JL26_RS08165 (JL26_08405) - 1631713..1632684 (+) 972 WP_012621529.1 IS110 family transposase -
  JL26_RS08170 (JL26_08410) gmk 1632753..1633379 (+) 627 WP_021113158.1 guanylate kinase -
  JL26_RS08175 (JL26_08415) radA 1633432..1634808 (-) 1377 WP_021114521.1 DNA repair protein RadA -
  JL26_RS08180 (JL26_08420) pilA 1634996..1635454 (+) 459 WP_038514693.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  JL26_RS08185 (JL26_08425) pilB 1635502..1636887 (+) 1386 WP_038514696.1 GspE/PulE family protein Machinery gene
  JL26_RS11470 (JL26_08430) - 1636880..1638075 (+) 1196 Protein_1640 type II secretion system F family protein -
  JL26_RS08200 (JL26_08435) - 1638072..1638740 (+) 669 WP_038514704.1 prepilin peptidase -
  JL26_RS08205 (JL26_08440) dapE 1638824..1639957 (+) 1134 WP_038514707.1 succinyl-diaminopimelate desuccinylase -
  JL26_RS08210 (JL26_08445) clpX 1640031..1641281 (-) 1251 WP_038514710.1 ATP-dependent protease ATP-binding subunit ClpX -
  JL26_RS08215 (JL26_08450) clpP 1641288..1641869 (-) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52716.54 Da        Isoelectric Point: 4.9814

>NTDB_id=127128 JL26_RS08185 WP_038514696.1 1635502..1636887(+) (pilB) [Glaesserella parasuis strain SH03]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFVEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGIGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=127128 JL26_RS08185 WP_038514696.1 1635502..1636887(+) (pilB) [Glaesserella parasuis strain SH03]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGTTGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAACAAAAATTGATTATTCGTCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCATAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGACTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

97.18

100

0.972
  pilB Haemophilus influenzae Rd KW20

57.802

98.698

0.57
  pilB Haemophilus influenzae 86-028NP

57.081

99.566

0.568
  pilB Vibrio parahaemolyticus RIMD 2210633

40.125

100

0.419
  pilB Vibrio cholerae strain A1552

38.755

100

0.419
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.414
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Acinetobacter baumannii D1279779

35.992

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.675

100

0.393
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilF Neisseria gonorrhoeae MS11

36.402

100

0.377

Multiple sequence alignment