Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HW00_RS08710 Genome accession   NZ_CP008867
Coordinates   1895108..1897240 (-) Length   710 a.a.
NCBI ID   WP_059317118.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain T52373     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1890108..1902240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW00_RS08695 (HW00_08715) - 1891766..1893415 (-) 1650 WP_023103572.1 AAA family ATPase -
  HW00_RS08700 (HW00_08720) aroB 1893422..1894528 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  HW00_RS08705 (HW00_08725) aroK 1894578..1895096 (-) 519 WP_003095833.1 shikimate kinase AroK -
  HW00_RS08710 (HW00_08730) pilQ 1895108..1897240 (-) 2133 WP_059317118.1 type 4a pilus secretin PilQ Machinery gene
  HW00_RS08715 (HW00_08735) pilP 1897294..1897818 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  HW00_RS08720 (HW00_08740) pilO 1897815..1898438 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  HW00_RS08725 (HW00_08745) pilN 1898435..1899031 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  HW00_RS08730 (HW00_08750) pilM 1899031..1900095 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 77416.97 Da        Isoelectric Point: 5.4402

>NTDB_id=125128 HW00_RS08710 WP_059317118.1 1895108..1897240(-) (pilQ) [Pseudomonas aeruginosa strain T52373]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTRLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTMEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SSGEEKENSRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHNGNWNS
YGKNGNMGIKDKEGYNCGPFQGQCTFPTRDNSPTPFVDMGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEVVSQPKV
VTSDKETAKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFQNALNGVPPINKNEVNA
KILVNDGETIVIGGVFSNTQTKAVDKVPFLGDLPYLGRLFRRDTVSDTKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=125128 HW00_RS08710 WP_059317118.1 1895108..1897240(-) (pilQ) [Pseudomonas aeruginosa strain T52373]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCGTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGTTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
TTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACGCGGTTGATCATCAACCTGACCAGGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCTGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATTCAAGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAACCGATGACCATGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCTGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATTGCCAAGCTGTTCCAGTCGGTTACT
AGTAGTGGCGAAGAGAAAGAAAATTCTCGTGGTTCGATTACCGTAGATGACCGTACCAACAGCATCATTGCCTATCAGCC
GCAGGAGCGGCTGGACGAGCTACGCCGAATCGTTTCCCAGTTGGACATCCCGGTTCGTCAGGTGATGATCGAGGCCCGTA
TCGTCGAGGCCAACGTGGGGTACGACAAGAGTCTTGGTGTGCGTTGGGGTGGTGCCTACCACAATGGCAACTGGAACAGC
TACGGCAAGAACGGCAACATGGGCATCAAGGATAAGGAAGGTTACAACTGCGGGCCGTTCCAGGGGCAGTGTACGTTCCC
GACCAGAGATAATTCTCCGACCCCATTTGTGGATATGGGGGCCAAGGATGCTACTTCCGGTATCGGTATTGGCTTTATCA
CCGATAACATCATTCTTGACTTGCAATTATCGGCCATGGAGAAAACCGGGAACGGCGAAGTGGTTTCGCAACCCAAGGTC
GTAACTTCTGACAAGGAAACCGCGAAGATCCTAAAGGGATCGGAGATCCCATATCAGGAGGCCAGTTCCAGTGGTGCGAC
CAGCACCTCGTTCAAGGAGGCCGCTCTTTCTCTGGAGGTGACGCCGCAGATCACTCCAGACAATCGGATCATCGTTGAGG
TGAAGGTGACCAAGGATGCTCCCGACTTCCAGAATGCTTTGAATGGAGTTCCTCCGATCAACAAGAACGAGGTTAACGCC
AAGATTCTGGTCAACGATGGTGAAACCATCGTTATCGGCGGTGTATTCAGCAACACCCAGACAAAGGCTGTCGACAAGGT
ACCCTTCCTCGGTGACCTGCCTTACTTGGGCCGTTTGTTCCGCCGCGATACGGTCTCCGACACGAAAAACGAACTTCTGG
TTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(545-701)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-447)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(307-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

94.678

100

0.952
  comQ Acinetobacter baylyi ADP1

42.368

98.732

0.418
  pilQ Acinetobacter baumannii strain A118

40.621

99.859

0.406
  pilQ Acinetobacter baumannii D1279779

40.621

99.859

0.406
  pilQ Legionella pneumophila strain Lp02

38.248

96.479

0.369
  pilQ Legionella pneumophila strain ERS1305867

38.248

96.479

0.369

Multiple sequence alignment