NTDB

Overview

Name	comEA/celA/cilE	Type	Machinery gene
Locus tag	CUGBS98_RS03910	Genome accession	NZ_CP010875
Coordinates	736814..737467 (+)	Length	217 a.a.
NCBI ID	WP_000461744.1	Uniprot ID	Q8E0F2
Organism	Streptococcus agalactiae strain CU_GBS_98
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 731814..742467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUGBS98_RS03885 (CUGBS98_00777)	-	731980..732810 (+)	831	WP_000735507.1	MetQ/NlpA family ABC transporter substrate-binding protein	-
CUGBS98_RS03890 (CUGBS98_00778)	-	733045..734631 (+)	1587	WP_000673089.1	DEAD/DEAH box helicase	-
CUGBS98_RS03895 (CUGBS98_00779)	-	734816..735082 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
CUGBS98_RS03900 (CUGBS98_00780)	-	735075..735839 (-)	765	WP_000567425.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
CUGBS98_RS03905 (CUGBS98_00781)	-	735974..736714 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
CUGBS98_RS03910 (CUGBS98_00782)	comEA/celA/cilE	736814..737467 (+)	654	WP_000461744.1	helix-hairpin-helix domain-containing protein	Machinery gene
CUGBS98_RS03915 (CUGBS98_00783)	comEC/celB	737451..739688 (+)	2238	WP_017647796.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CUGBS98_RS03920 (CUGBS98_00784)	-	739814..740623 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
CUGBS98_RS03925 (CUGBS98_00785)	-	740634..741578 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-

Sequence

Protein

Download Length: 217 a.a. Molecular weight: 23713.07 Da Isoelectric Point: 9.5543

                                >NTDB_id=125042 CUGBS98_RS03910 WP_000461744.1 736814..737467(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CU_GBS_98]

MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA

VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK

ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide

Download Length: 654 bp

                                >NTDB_id=125042 CUGBS98_RS03910 WP_000461744.1 736814..737467(+) (comEA/celA/cilE) [Streptococcus agalactiae strain CU_GBS_98]

ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG

AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACAATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA

TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA

GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA

TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA

AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCTACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA

GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGGGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA

AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG

TTACAATTGACTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(75-128)

Helix-hairpin-helix motif (HHH_3)

(153-215)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8E0F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45	100	0.456
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.694	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae D39	43.694	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae R6	43.694	100	0.447
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.243	100	0.442
comEA/celA/cilE	Streptococcus mitis SK321	43.636	100	0.442
comEA	Lactococcus lactis subsp. cremoris KW2	39.013	100	0.401
comEA	Streptococcus thermophilus LMD-9	54.545	70.968	0.387

Detailed information NTG0125042