Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSA3335_RS26395 Genome accession   NZ_CP008742
Coordinates   5521680..5523785 (-) Length   701 a.a.
NCBI ID   WP_002551656.1    Uniprot ID   A0AB35R090
Organism   Pseudomonas savastanoi pv. savastanoi NCPPB 3335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5516680..5528785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSA3335_RS26380 (PSA3335_25265) - 5518314..5519927 (-) 1614 WP_002551659.1 SPOR domain-containing protein -
  PSA3335_RS26385 (PSA3335_25270) aroB 5519938..5521041 (-) 1104 WP_002551658.1 3-dehydroquinate synthase -
  PSA3335_RS26390 (PSA3335_25275) aroK 5521157..5521675 (-) 519 WP_002551657.1 shikimate kinase AroK -
  PSA3335_RS26395 (PSA3335_25280) pilQ 5521680..5523785 (-) 2106 WP_002551656.1 type IV pilus secretin PilQ Machinery gene
  PSA3335_RS26400 (PSA3335_25285) pilP 5523833..5524360 (-) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  PSA3335_RS26405 (PSA3335_25290) pilO 5524357..5524980 (-) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  PSA3335_RS26410 (PSA3335_25295) - 5524977..5525576 (-) 600 WP_002551653.1 PilN domain-containing protein -
  PSA3335_RS26415 (PSA3335_25300) pilM 5525576..5526640 (-) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75543.91 Da        Isoelectric Point: 5.0976

>NTDB_id=123998 PSA3335_RS26395 WP_002551656.1 5521680..5523785(-) (pilQ) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEASNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNALLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=123998 PSA3335_RS26395 WP_002551656.1 5521680..5523785(-) (pilQ) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTAAA
GACTCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGAGAA
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATCAACCTGACCACGCT
TTCGCCCTACAGCTCCCGCGTCGACGGCAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAGACCGTTTATCCCCGCG
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGATTTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATTCGCGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGCGACAAGGCCAGTATC
CTGATCGAACCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGTGTACGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGACACGGTTCAGGGCGGTATT
ACCCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCCGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
AACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAAGTAACACTGGCAGCAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACTGTGAGCTTCAAGGAGGCCTCGCT
GTCGCTTGAGGTAACGCCACAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGTTGCTTGGTGTACCACCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.784

100

0.74
  comQ Acinetobacter baylyi ADP1

40.599

100

0.425
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.405
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.405
  pilQ Legionella pneumophila strain Lp02

38.495

98.573

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.406

98.431

0.378

Multiple sequence alignment