Detailed information NTG0123999

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PSA3335_RS26415
• Genome accession: NZ_CP008742
• Coordinates: 5525576..5526640 (-)
• Length: 354 a.a.
• NCBI ID: WP_002551652.1
• Uniprot ID: A0A7Z6Y2Y4
• Organism: Pseudomonas savastanoi pv. savastanoi NCPPB 3335
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5520576..5531640

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSA3335_RS26390 (PSA3335_25275)	aroK	5521157..5521675 (-)	519	WP_002551657.1	shikimate kinase AroK	-
PSA3335_RS26395 (PSA3335_25280)	pilQ	5521680..5523785 (-)	2106	WP_002551656.1	type IV pilus secretin PilQ	Machinery gene
PSA3335_RS26400 (PSA3335_25285)	pilP	5523833..5524360 (-)	528	WP_002551655.1	type 4a pilus biogenesis lipoprotein PilP	-
PSA3335_RS26405 (PSA3335_25290)	pilO	5524357..5524980 (-)	624	WP_002551654.1	type 4a pilus biogenesis protein PilO	-
PSA3335_RS26410 (PSA3335_25295)	-	5524977..5525576 (-)	600	WP_002551653.1	PilN domain-containing protein	-
PSA3335_RS26415 (PSA3335_25300)	pilM	5525576..5526640 (-)	1065	WP_002551652.1	pilus assembly protein PilM	Machinery gene
PSA3335_RS26420 (PSA3335_25305)	-	5526850..5529291 (+)	2442	WP_369812045.1	penicillin-binding protein 1A	-
PSA3335_RS26425 (PSA3335_25310)	-	5529564..5530832 (-)	1269	WP_002551650.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38078.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=123999 PSA3335_RS26415 WP_002551652.1 5525576..5526640(-) (pilM) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=123999 PSA3335_RS26415 WP_002551652.1 5525576..5526640(-) (pilM) [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCGTTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGCGTGCTCGCCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTC
GCCGTAGCCGGTTCCGCCGTGATCACCAAGACGATTGAAATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAGGCTGATCAGTACATCCCTTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAACGCGTCGAAGTATTGCTGGCTGCCTGCCGCAAAGAGAATGTCGAAGTGCGTGAGGCGGCGCTGGCTCTG
GCCGGGCTGACCGCGCGCGTGGTGGACGTCGAGGCCTATGCCCTGGAGCGCTCGTTCGGCCTGCTGGCTGCGCAACTGGG
TAACGGTCATGACGAGCTTACCGTGGCGGTGGTCGATATCGGTGCCACCATGACCACCCTCAGCGTGCTGCATCATGGCC
GGATCATCTATACCCGCGAGCAACTGTTTGGTGGCCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTTTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTCAGCGAGGTGCTGGACCCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCTCGTTCTTTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAACGACGCATTGGCACCCCGACGATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCTCCAAGGTCAATGCCGGGGCGCTGGCCAGTGACGCTCCCGCGTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A7Z6Y2Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469