Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   EP54_RS10355 Genome accession   NZ_CP007670
Coordinates   2036088..2036513 (-) Length   141 a.a.
NCBI ID   WP_047335183.1    Uniprot ID   A0A2C9TRG5
Organism   Staphylococcus aureus strain M121     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2000279..2044987 2036088..2036513 within 0


Gene organization within MGE regions


Location: 2000279..2044987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP54_RS10090 (EP54_09580) - 2000279..2000794 (-) 516 WP_000163283.1 type 1 glutamine amidotransferase domain-containing protein -
  EP54_RS16240 - 2000925..2001086 (-) 162 WP_001005407.1 SE1561 family protein -
  EP54_RS17025 - 2001433..2001513 (+) 81 WP_100250272.1 hypothetical protein -
  EP54_RS10100 (EP54_09590) - 2002157..2003602 (-) 1446 WP_001148112.1 SH3 domain-containing protein -
  EP54_RS10105 (EP54_09595) - 2003583..2004020 (-) 438 WP_000354132.1 phage holin -
  EP54_RS10110 (EP54_09600) - 2004076..2004471 (-) 396 WP_001604146.1 hypothetical protein -
  EP54_RS10115 (EP54_09605) - 2004476..2005714 (-) 1239 WP_023487053.1 BppU family phage baseplate upper protein -
  EP54_RS10120 (EP54_09610) - 2005727..2007601 (-) 1875 WP_047335170.1 glucosaminidase domain-containing protein -
  EP54_RS10125 (EP54_09615) - 2007738..2008037 (-) 300 WP_000466784.1 DUF2951 domain-containing protein -
  EP54_RS10130 - 2008078..2008251 (-) 174 WP_015990323.1 XkdX family protein -
  EP54_RS10135 (EP54_09625) - 2008261..2008638 (-) 378 WP_024928064.1 DUF2977 domain-containing protein -
  EP54_RS10140 (EP54_09630) - 2008638..2010461 (-) 1824 WP_031925170.1 phage baseplate upper protein -
  EP54_RS10145 (EP54_09635) - 2010461..2012359 (-) 1899 WP_047335171.1 hypothetical protein -
  EP54_RS10150 (EP54_09640) - 2012372..2014258 (-) 1887 WP_001604154.1 SGNH/GDSL hydrolase family protein -
  EP54_RS10155 (EP54_09645) - 2014269..2015210 (-) 942 WP_047335172.1 phage tail domain-containing protein -
  EP54_RS10160 (EP54_09650) - 2015225..2018110 (-) 2886 WP_047335173.1 terminase -
  EP54_RS10165 (EP54_09655) - 2018114..2018398 (-) 285 WP_000880587.1 hypothetical protein -
  EP54_RS10170 (EP54_09660) - 2018443..2018949 (-) 507 WP_000134337.1 tail assembly chaperone -
  EP54_RS10175 (EP54_09665) - 2019016..2019573 (-) 558 WP_000057582.1 tail protein -
  EP54_RS10180 (EP54_09670) - 2019574..2019999 (-) 426 WP_000270190.1 DUF3168 domain-containing protein -
  EP54_RS10185 - 2020012..2020419 (-) 408 WP_001151323.1 HK97-gp10 family putative phage morphogenesis protein -
  EP54_RS10190 (EP54_09680) - 2020406..2020741 (-) 336 WP_000483041.1 phage head closure protein -
  EP54_RS10195 (EP54_09685) - 2020753..2021103 (-) 351 WP_000177351.1 phage head-tail adapter protein -
  EP54_RS17140 - 2021109..2021252 (-) 144 WP_000002931.1 hypothetical protein -
  EP54_RS10200 (EP54_09695) - 2021264..2022178 (-) 915 WP_000235168.1 phage major capsid protein -
  EP54_RS10205 (EP54_09700) - 2022195..2022779 (-) 585 WP_001019221.1 DUF4355 domain-containing protein -
  EP54_RS10210 (EP54_09705) - 2022875..2023825 (-) 951 WP_001604161.1 phage head morphogenesis protein -
  EP54_RS10215 (EP54_09710) - 2023794..2025218 (-) 1425 WP_000177426.1 phage portal protein -
  EP54_RS10220 (EP54_09715) - 2025215..2026438 (-) 1224 WP_001037578.1 PBSX family phage terminase large subunit -
  EP54_RS10225 (EP54_09720) - 2026431..2026925 (-) 495 WP_000594079.1 terminase small subunit -
  EP54_RS10235 - 2027280..2027681 (-) 402 WP_000286968.1 hypothetical protein -
  EP54_RS10240 (EP54_09730) rinB 2027682..2027855 (-) 174 WP_001619900.1 transcriptional activator RinB -
  EP54_RS10245 (EP54_09735) - 2027852..2028241 (-) 390 WP_001662395.1 hypothetical protein -
  EP54_RS10250 (EP54_09740) - 2028231..2028467 (-) 237 WP_000608281.1 hypothetical protein -
  EP54_RS10255 (EP54_09745) - 2028460..2028663 (-) 204 WP_001072795.1 hypothetical protein -
  EP54_RS10260 (EP54_09750) - 2028660..2028854 (-) 195 WP_000195798.1 DUF1381 domain-containing protein -
  EP54_RS10265 (EP54_09755) - 2028851..2029096 (-) 246 WP_000120375.1 hypothetical protein -
  EP54_RS10270 (EP54_09760) - 2029133..2029642 (-) 510 WP_000185637.1 dUTP diphosphatase -
  EP54_RS16250 - 2029635..2029805 (-) 171 WP_000714409.1 hypothetical protein -
  EP54_RS10280 (EP54_09770) - 2029792..2030046 (-) 255 WP_047335174.1 DUF1024 family protein -
  EP54_RS10285 (EP54_09775) - 2030039..2030428 (-) 390 WP_031899414.1 hypothetical protein -
  EP54_RS10290 (EP54_09780) - 2030425..2030772 (-) 348 WP_047335175.1 YopX family protein -
  EP54_RS10295 (EP54_09785) - 2030769..2031173 (-) 405 WP_000695756.1 hypothetical protein -
  EP54_RS10300 (EP54_09790) - 2031176..2031382 (-) 207 WP_000693989.1 hypothetical protein -
  EP54_RS10305 (EP54_09795) - 2031396..2031638 (-) 243 WP_047335176.1 SAV1978 family virulence-associated passenger protein -
  EP54_RS10310 (EP54_09800) - 2031642..2031989 (-) 348 Protein_1951 SA1788 family PVL leukocidin-associated protein -
  EP54_RS10315 (EP54_09805) - 2031990..2032175 (-) 186 WP_047335177.1 DUF3113 family protein -
  EP54_RS10320 (EP54_09810) - 2032180..2032584 (-) 405 WP_015973976.1 DUF1064 domain-containing protein -
  EP54_RS10325 (EP54_09815) - 2032595..2032816 (-) 222 WP_047335178.1 DUF3269 family protein -
  EP54_RS10330 (EP54_09820) - 2032819..2033034 (-) 216 WP_021285875.1 hypothetical protein -
  EP54_RS10335 (EP54_09825) - 2033031..2034272 (-) 1242 WP_047335179.1 DnaB helicase C-terminal domain-containing protein -
  EP54_RS10340 (EP54_09830) - 2034269..2034625 (-) 357 WP_047335180.1 hypothetical protein -
  EP54_RS10345 (EP54_09835) - 2034625..2035407 (-) 783 WP_047335181.1 phage replisome organizer N-terminal domain-containing protein -
  EP54_RS10350 (EP54_09840) - 2035379..2036074 (-) 696 WP_047335182.1 putative HNHc nuclease -
  EP54_RS10355 (EP54_09845) ssbA 2036088..2036513 (-) 426 WP_047335183.1 single-stranded DNA-binding protein Machinery gene
  EP54_RS10360 (EP54_09850) - 2036513..2037136 (-) 624 WP_047335184.1 DUF1071 domain-containing protein -
  EP54_RS10365 (EP54_09855) - 2037137..2037673 (-) 537 WP_047335185.1 host-nuclease inhibitor Gam family protein -
  EP54_RS10370 (EP54_09860) - 2037666..2037902 (-) 237 WP_047335186.1 hypothetical protein -
  EP54_RS10375 (EP54_09865) - 2037910..2038170 (-) 261 WP_000291075.1 DUF1108 family protein -
  EP54_RS10380 (EP54_09870) - 2038264..2038584 (-) 321 WP_000219666.1 hypothetical protein -
  EP54_RS16255 - 2038585..2038752 (-) 168 WP_047335187.1 DUF1270 domain-containing protein -
  EP54_RS10390 (EP54_09880) - 2038745..2038960 (-) 216 WP_025175692.1 hypothetical protein -
  EP54_RS10395 - 2039031..2039696 (+) 666 WP_031764600.1 hypothetical protein -
  EP54_RS17220 - 2039717..2039785 (-) 69 Protein_1969 hypothetical protein -
  EP54_RS10400 (EP54_09895) - 2039801..2040550 (-) 750 WP_001148587.1 phage antirepressor KilAC domain-containing protein -
  EP54_RS10405 (EP54_09900) - 2040552..2040737 (-) 186 WP_000933365.1 helix-turn-helix domain-containing protein -
  EP54_RS17280 - 2041180..2041314 (+) 135 WP_001119050.1 hypothetical protein -
  EP54_RS10410 (EP54_09910) - 2041311..2041514 (-) 204 WP_031764597.1 hypothetical protein -
  EP54_RS10415 (EP54_09915) - 2041528..2041764 (-) 237 WP_001121116.1 helix-turn-helix domain-containing protein -
  EP54_RS10420 (EP54_09920) - 2041915..2042229 (+) 315 WP_000333630.1 helix-turn-helix domain-containing protein -
  EP54_RS10425 (EP54_09925) - 2042242..2042703 (+) 462 WP_001573840.1 phage protein -
  EP54_RS10435 (EP54_09935) - 2042887..2043408 (+) 522 WP_001102444.1 hypothetical protein -
  EP54_RS10440 (EP54_09940) - 2043401..2043871 (+) 471 WP_000183249.1 hypothetical protein -
  EP54_RS10445 (EP54_09945) - 2043941..2044987 (+) 1047 WP_001145732.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15876.39 Da        Isoelectric Point: 5.1746

>NTDB_id=122318 EP54_RS10355 WP_047335183.1 2036088..2036513(-) (ssbA) [Staphylococcus aureus strain M121]
MLNRVVLVGRLTKDPELRSTPNGVNVGTFTLAVNRTFTNAQGEREADFINCVTFRKQADNVNNYLSKGSLAGVDGRLQSR
SYENQEGRRVFVTEVVCDSVQFLEPKNNNQQPNNNYHQQGQTQTGNNPFDNTETDFSDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=122318 EP54_RS10355 WP_047335183.1 2036088..2036513(-) (ssbA) [Staphylococcus aureus strain M121]
ATGTTAAACAGAGTAGTTTTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCACGCCAAATGGCGTAAATGTAGG
GACGTTCACATTAGCAGTAAACAGAACATTCACAAACGCGCAAGGAGAACGTGAGGCAGATTTTATTAATTGTGTAACTT
TTAGAAAACAAGCAGATAACGTGAATAACTATTTATCAAAAGGATCATTAGCTGGTGTTGACGGACGCCTACAATCACGT
AGTTATGAAAATCAAGAAGGTCGTCGTGTATTTGTTACCGAAGTTGTATGTGACAGTGTCCAATTCCTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAGGACAAACTCAAACTGGTAATAATCCATTTGACAATACTGAAA
CAGATTTTTCAGACCTCCCATTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2C9TRG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

75.424

83.688

0.631

  ssb Latilactobacillus sakei subsp. sakei 23K

54.605

100

0.589

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

75.177

0.454

  ssbB Streptococcus sobrinus strain NIDR 6715-7

38.298

100

0.383

  ssbA Streptococcus mutans UA159

44.348

81.56

0.362

  ssbB/cilA Streptococcus mitis NCTC 12261

43.22

83.688

0.362

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.22

83.688

0.362


Multiple sequence alignment