Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SH08_RS20925 Genome accession   NZ_CP010440
Coordinates   4201002..4201679 (+) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli K-12 strain K-12 MG1655     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4196002..4206679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH08_RS20890 (SH08_20905) gspG 4196482..4196919 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  SH08_RS20895 (SH08_20910) gspH 4196927..4197436 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  SH08_RS20900 (SH08_20915) gspI 4197433..4197810 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SH08_RS20905 (SH08_20920) gspJ 4197803..4198390 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SH08_RS20910 (SH08_20925) gspK 4198383..4199366 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  SH08_RS20915 (SH08_20930) gspL 4199381..4200544 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  SH08_RS20920 (SH08_20935) gspM 4200541..4201002 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  SH08_RS20925 (SH08_20940) pilD 4201002..4201679 (+) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  SH08_RS20930 (SH08_20945) bfr 4201708..4202184 (-) 477 WP_000675504.1 bacterioferritin -
  SH08_RS20935 (SH08_20950) bfd 4202256..4202450 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SH08_RS20940 (SH08_20955) chiA 4202619..4205312 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=120838 SH08_RS20925 WP_000178154.1 4201002..4201679(+) (pilD) [Escherichia coli K-12 strain K-12 MG1655]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=120838 SH08_RS20925 WP_000178154.1 4201002..4201679(+) (pilD) [Escherichia coli K-12 strain K-12 MG1655]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment