Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   GBS85147_RS03815 Genome accession   NZ_CP010319
Coordinates   726785..727438 (+) Length   217 a.a.
NCBI ID   WP_000461740.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721785..732438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS03790 (GBS85147_0768) - 721952..722782 (+) 831 WP_000735512.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  GBS85147_RS03795 (GBS85147_0769) - 723017..724603 (+) 1587 WP_000673086.1 DEAD/DEAH box helicase -
  GBS85147_RS03800 (GBS85147_0770) - 724788..725054 (-) 267 WP_000598736.1 GIY-YIG nuclease family protein -
  GBS85147_RS03805 (GBS85147_0771) - 725047..725811 (-) 765 WP_000567424.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  GBS85147_RS03810 (GBS85147_0772) - 725946..726686 (+) 741 WP_000500221.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  GBS85147_RS03815 (GBS85147_0773) comEA/celA/cilE 726785..727438 (+) 654 WP_000461740.1 helix-hairpin-helix domain-containing protein Machinery gene
  GBS85147_RS03820 (GBS85147_0774) comEC/celB 727422..729659 (+) 2238 WP_000939914.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GBS85147_RS03825 (GBS85147_0775) - 729784..730593 (+) 810 WP_000153216.1 Cof-type HAD-IIB family hydrolase -
  GBS85147_RS03830 (GBS85147_0776) - 730604..731548 (+) 945 WP_000200826.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 23714.05 Da        Isoelectric Point: 9.2366

>NTDB_id=120072 GBS85147_RS03815 WP_000461740.1 726785..727438(+) (comEA/celA/cilE) [Streptococcus agalactiae strain GBS85147]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide


Download         Length: 654 bp        

>NTDB_id=120072 GBS85147_RS03815 WP_000461740.1 726785..727438(+) (comEA/celA/cilE) [Streptococcus agalactiae strain GBS85147]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTGTTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACGATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCCACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGAGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(153-215)

SLBB domain (SLBB)

(75-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

44.595

100

0.456
  comEA/celA/cilE Streptococcus mitis SK321

44.144

100

0.452
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae D39

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae R6

43.694

100

0.447
  comEA/celA/cilE Streptococcus mitis NCTC 12261

43.243

100

0.442
  comEA Lactococcus lactis subsp. cremoris KW2

39.013

100

0.401
  comEA Streptococcus thermophilus LMD-9

54.545

70.968

0.387