Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOQOE_RS04495 Genome accession   NZ_OZ245004
Coordinates   972567..974300 (-) Length   577 a.a.
NCBI ID   WP_420554989.1    Uniprot ID   -
Organism   MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 967567..979300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQOE_RS04470 - 968653..969606 (+) 954 WP_420554985.1 Nudix family hydrolase -
  ACOQOE_RS04475 yacG 969575..969811 (-) 237 WP_420554986.1 DNA gyrase inhibitor YacG -
  ACOQOE_RS04480 coaE 969804..970415 (-) 612 WP_420554987.1 dephospho-CoA kinase -
  ACOQOE_RS04485 pilD 970416..971261 (-) 846 WP_420554988.1 prepilin peptidase Machinery gene
  ACOQOE_RS04490 pilC 971328..972548 (-) 1221 WP_067862381.1 type II secretion system F family protein Machinery gene
  ACOQOE_RS04495 pilB 972567..974300 (-) 1734 WP_420554989.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOQOE_RS04500 - 974474..974899 (+) 426 WP_420554990.1 pilin -
  ACOQOE_RS04505 - 974991..975332 (+) 342 WP_420554991.1 hypothetical protein -
  ACOQOE_RS04510 nadC 975458..976303 (-) 846 WP_420554992.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOQOE_RS04515 ampD 976418..976987 (+) 570 WP_420555460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOQOE_RS04520 - 976973..977683 (-) 711 WP_067862372.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACOQOE_RS04525 - 977885..978922 (+) 1038 WP_231867910.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63708.79 Da        Isoelectric Point: 4.8689

>NTDB_id=1171216 ACOQOE_RS04495 WP_420554989.1 972567..974300(-) (pilB) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
MIVRGYILQPSQPLSGLAKRLVNEGVCSAEVAADAALQCREKQQTFIAYVIEQRLVSAYRAAAAASEEFGIPLLDLDAMD
MATLPQGLINDSLISKHHALPLQKRDNRLFVAISDPTNIQALDEFKFQSGITTEAIIVEEDKLTKAIESFLDSQDSALDD
LEDSDLDNLEIDDSLNNAPQEESAEDAANDAPIVRFVNKILLDSIKNGASDIHFEPYEKTYRIRSRIDGILQEIAKPPSN
LSSRLSARLKVMAQMDISERRVPQDGRIKMKISKNRAIDFRVNTLPTLWGEKIVLRILDPSSAQMGVEALGFADFQKEMY
LSTLHKPQGMVLVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVHVSSKVGLTFAEALRAFLRQDPDV
VMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLGRRLCESCKQPITLPEV
TLREEGFTDEEIKNMTLFEANHEGCNKCNRGYKGRVGIYEMLAMSPEIAEVIMQNGNSLDILKVANTQGFQSLRRSGLKK
VAEGVTSLEEMNRVIKV

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1171216 ACOQOE_RS04495 WP_420554989.1 972567..974300(-) (pilB) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
ATGATAGTAAGGGGTTATATTTTGCAGCCTAGCCAACCATTAAGCGGACTAGCAAAAAGGTTAGTTAATGAGGGTGTTTG
CTCTGCAGAGGTCGCGGCCGATGCGGCGCTTCAGTGCCGAGAAAAACAGCAAACCTTTATCGCTTATGTTATTGAACAGC
GCCTTGTTAGTGCCTATAGAGCCGCCGCAGCCGCATCCGAAGAGTTTGGCATTCCACTATTAGATTTGGATGCCATGGAC
ATGGCCACGCTTCCCCAAGGGCTAATTAATGACTCGCTGATCAGTAAGCATCACGCACTCCCGCTACAAAAAAGAGATAA
TCGCTTATTTGTAGCTATATCTGACCCAACCAATATTCAGGCATTAGACGAATTTAAGTTTCAAAGTGGTATCACCACTG
AAGCGATTATTGTTGAAGAAGACAAGCTTACCAAAGCGATAGAGTCCTTTCTCGACAGTCAAGATTCAGCCTTAGATGAC
TTAGAAGATAGCGACCTTGATAATTTAGAGATTGATGATTCGCTAAATAATGCGCCACAAGAAGAATCCGCAGAAGATGC
TGCCAATGACGCCCCCATTGTTCGCTTTGTAAATAAAATATTGCTGGATTCCATTAAGAACGGCGCCTCAGATATACACT
TTGAACCCTACGAAAAAACCTATCGTATACGAAGCCGTATCGATGGAATTTTACAAGAAATTGCGAAACCTCCATCTAAT
TTATCATCACGTCTATCGGCTCGTTTAAAAGTTATGGCCCAGATGGATATTTCTGAACGCCGCGTCCCCCAAGATGGACG
CATCAAGATGAAGATATCCAAAAATAGAGCGATCGATTTTCGTGTTAACACCCTACCCACCCTCTGGGGTGAAAAGATCG
TACTACGTATATTAGACCCGAGCAGTGCTCAAATGGGGGTTGAGGCTTTAGGGTTCGCTGATTTCCAAAAAGAGATGTAC
CTTTCCACCTTACATAAACCACAAGGCATGGTACTCGTCACAGGCCCTACCGGAAGTGGTAAAACCGTTAGTCTTTATAC
AGGTCTAAATATACTTAATACTGAAGAGCGCAATATATCTACGGCTGAAGACCCTGTAGAGATTAACCTAGAAGGCATCA
ACCAAGTTCATGTAAGCTCAAAAGTAGGCCTTACCTTTGCGGAAGCGCTACGCGCCTTCCTCCGCCAAGACCCTGATGTA
GTGATGGTGGGTGAGATTCGAGATCTTGAAACCGCAGAAATTGCGATTAAAGCCGCACAAACCGGCCATATGGTGCTCTC
CACGCTACACACAAACAGCGCCCCAGAAACCCTAACCCGACTAAGAAACATGGGCGTACCCGCCTTTAATATTGCCACTT
CAGTGAGTTTAATTATCGCCCAACGATTGGGCCGACGCCTCTGCGAAAGCTGTAAACAACCCATTACACTACCTGAAGTT
ACCCTACGTGAAGAGGGCTTTACCGACGAAGAGATCAAGAATATGACCCTCTTCGAAGCTAATCATGAGGGTTGCAATAA
GTGTAACAGAGGTTATAAAGGTAGGGTTGGTATCTACGAGATGCTTGCTATGTCCCCAGAGATTGCTGAGGTCATTATGC
AAAACGGAAACTCTCTGGATATACTAAAGGTCGCCAACACTCAAGGATTCCAAAGCTTAAGACGTTCAGGTCTGAAGAAA
GTTGCCGAAGGTGTGACTAGCCTCGAAGAGATGAATCGCGTCATTAAGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-156)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.469

98.614

0.567
  pilB Acinetobacter baylyi ADP1

57.726

97.574

0.563
  pilB Legionella pneumophila strain ERS1305867

54.24

98.094

0.532
  pilB Vibrio cholerae strain A1552

54.932

89.601

0.492
  pilB Vibrio campbellii strain DS40M4

53.204

89.255

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

52.93

88.735

0.47
  pilF Neisseria gonorrhoeae MS11

47.527

98.094

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

40.546

88.908

0.36

Multiple sequence alignment