Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACOQOE_RS04485 Genome accession   NZ_OZ245004
Coordinates   970416..971261 (-) Length   281 a.a.
NCBI ID   WP_420554988.1    Uniprot ID   -
Organism   MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965416..976261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQOE_RS04465 argJ 967381..968601 (+) 1221 WP_420554984.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACOQOE_RS04470 - 968653..969606 (+) 954 WP_420554985.1 Nudix family hydrolase -
  ACOQOE_RS04475 yacG 969575..969811 (-) 237 WP_420554986.1 DNA gyrase inhibitor YacG -
  ACOQOE_RS04480 coaE 969804..970415 (-) 612 WP_420554987.1 dephospho-CoA kinase -
  ACOQOE_RS04485 pilD 970416..971261 (-) 846 WP_420554988.1 prepilin peptidase Machinery gene
  ACOQOE_RS04490 pilC 971328..972548 (-) 1221 WP_067862381.1 type II secretion system F family protein Machinery gene
  ACOQOE_RS04495 pilB 972567..974300 (-) 1734 WP_420554989.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOQOE_RS04500 - 974474..974899 (+) 426 WP_420554990.1 pilin -
  ACOQOE_RS04505 - 974991..975332 (+) 342 WP_420554991.1 hypothetical protein -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 31005.76 Da        Isoelectric Point: 5.9014

>NTDB_id=1171214 ACOQOE_RS04485 WP_420554988.1 970416..971261(-) (pilD) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
MILNIDPAWAIACAFILSLCVGSFLNVVIHRLPIMMEREWLAEHEAENTEQQEAFNLSAPASTCPQCGHKIRWYENIPVI
SYLYLRAKCASCGLHIPFRYPVIELLTAIAGAIIVYIYGVNLVSLSLTLLTWGLICLIFIDIDHQLLPDRITLPLLWLGL
FVNSFSLFTSLESAVYGAIAGYLSLWSVYWAFKLLTGKEGMGYGDFKLLAALGAWAGWAQLPLIILLSSLVGAILGIAMI
AAKKHEQQAPMPFGPYLAIAGWVALIWGDNITGLYLQFAGL

Nucleotide


Download         Length: 846 bp        

>NTDB_id=1171214 ACOQOE_RS04485 WP_420554988.1 970416..971261(-) (pilD) [MAG: Neptuniibacter marinus isolate 5ffc281b-0633-46d3-a470-07ce90f1f764]
ATGATCCTTAATATCGATCCAGCGTGGGCTATAGCCTGCGCTTTTATTTTATCCCTCTGCGTCGGCAGTTTTTTAAATGT
GGTTATTCACCGCCTACCTATTATGATGGAACGGGAATGGTTAGCTGAACATGAGGCAGAAAATACCGAACAACAGGAAG
CATTTAACCTTTCAGCGCCCGCCTCCACCTGCCCACAATGTGGGCATAAAATTAGATGGTACGAAAATATTCCTGTAATT
AGTTACCTATACCTAAGAGCCAAATGTGCTTCCTGCGGTTTACATATCCCTTTTCGTTACCCCGTTATTGAGCTATTAAC
CGCGATAGCAGGGGCTATTATTGTTTATATCTATGGTGTAAACCTAGTCAGCCTCAGCCTGACACTGCTGACCTGGGGGT
TAATCTGCCTAATCTTTATCGATATTGATCATCAATTATTACCTGACCGCATTACACTACCTTTACTGTGGTTAGGCTTA
TTCGTAAATAGCTTCAGCTTATTCACCTCTCTGGAAAGCGCTGTATATGGTGCTATTGCTGGCTACTTAAGTTTATGGAG
TGTTTATTGGGCGTTTAAATTATTAACAGGCAAAGAGGGTATGGGATACGGTGATTTTAAATTACTGGCCGCTCTCGGAG
CTTGGGCTGGATGGGCACAGTTACCCCTTATTATTCTATTGAGCTCTTTGGTTGGTGCTATATTAGGCATCGCGATGATC
GCAGCGAAAAAGCATGAACAACAAGCACCTATGCCGTTTGGCCCTTATCTCGCCATTGCCGGTTGGGTTGCCCTTATCTG
GGGCGATAATATTACCGGTCTCTACCTTCAATTTGCTGGTTTATAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(129-237)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(16-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

56.015

94.662

0.53
  pilD Acinetobacter nosocomialis M2

56.015

94.662

0.53
  pilD Vibrio cholerae strain A1552

54.074

96.085

0.52
  pilD Vibrio campbellii strain DS40M4

53.309

96.797

0.516
  pilD Neisseria gonorrhoeae MS11

47.445

97.509

0.463

Multiple sequence alignment