Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOOFO_RS09125 Genome accession   NZ_OZ244162
Coordinates   1982127..1983869 (+) Length   580 a.a.
NCBI ID   WP_420426473.1    Uniprot ID   -
Organism   MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1977127..1988869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOOFO_RS09105 csrA 1977864..1978061 (+) 198 WP_420426470.1 carbon storage regulator CsrA -
  ACOOFO_RS09115 - 1978879..1981116 (+) 2238 WP_420426471.1 hypothetical protein -
  ACOOFO_RS09120 - 1981161..1982018 (-) 858 WP_420426472.1 DUF4382 domain-containing protein -
  ACOOFO_RS09125 pilB 1982127..1983869 (+) 1743 WP_420426473.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOOFO_RS09130 pilC 1983895..1985112 (+) 1218 WP_420426474.1 type II secretion system F family protein Machinery gene
  ACOOFO_RS09135 - 1985116..1985964 (+) 849 WP_420426475.1 prepilin peptidase -
  ACOOFO_RS09140 coaE 1985964..1986605 (+) 642 WP_420426476.1 dephospho-CoA kinase -
  ACOOFO_RS09145 zapD 1986660..1987433 (+) 774 WP_420426477.1 cell division protein ZapD -
  ACOOFO_RS09150 - 1987430..1987654 (+) 225 WP_420426478.1 DNA gyrase inhibitor YacG -
  ACOOFO_RS09155 tadA 1987675..1988142 (+) 468 WP_420426479.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63237.98 Da        Isoelectric Point: 5.1903

>NTDB_id=1171038 ACOOFO_RS09125 WP_420426473.1 1982127..1983869(+) (pilB) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
MAVNPQNIPVSKGYLGGLARRIVAEGLLSEEQARTAQQKAQRSGQSLVSYAVEQKWAAARGLAMAASREFGVPLADLSAL
EVDNALASEVGEKVLRKHRALPLYRRGKRLFVAISDPSFNQALEEIKFATGQPTEGVLVEDDKLGKAMEAALSAVDAASL
SGDESEFDDIDLEAGGREEQAVDASATQSDADDAPVVRYVNKVLLDAIRRGASDIHFEPYEKYYRVRLRVDGELREFARP
PVQIAARLAARVKVLSRLDLAERRVPQDGRIKLKLSAQRAIDFRVSTCPTLWGEKIVLRILDSSSAQLGIESLGYEPEQR
ALYENALSKPQGMILVTGPTGSGKTVSLYTGLNILNTEGLNISTAEDPAEINLPGINQVNVNPRVGLTFAAALKAFLRQD
PDVIMVGEVRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLLNMGVPAYNIASSVSLIIAQRLAKRLCVHCRKEADI
PRVELLKEGFTEEQVDSEGFTIYEAQGCDECHSGYKGRTGIYQVMPVSETIGRMIMEGKNAIDVADQAEREGVLDLRQSA
LKKVRDGIIDLKEANRVTVE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1171038 ACOOFO_RS09125 WP_420426473.1 1982127..1983869(+) (pilB) [MAG: Algiphilus sp. isolate dd919ab0-ff71-4ff6-8152-ac5c3678e526]
ATGGCGGTCAATCCGCAGAATATCCCAGTCAGCAAAGGCTATCTTGGCGGTCTTGCGCGGCGTATCGTCGCCGAGGGACT
GCTGAGCGAAGAACAGGCGCGTACGGCCCAGCAGAAGGCGCAGCGGAGCGGGCAGTCGCTGGTCAGCTATGCGGTGGAGC
AGAAATGGGCAGCCGCCCGCGGCCTGGCCATGGCAGCCAGCCGCGAATTCGGCGTACCACTGGCCGATCTCTCGGCGCTT
GAGGTGGACAACGCACTGGCGAGCGAGGTGGGCGAGAAGGTCCTGCGCAAGCACCGCGCCCTGCCGCTGTACCGCCGCGG
TAAACGTCTGTTCGTGGCCATTTCGGATCCTTCCTTCAATCAGGCCCTGGAAGAGATCAAATTCGCCACAGGCCAGCCCA
CAGAGGGGGTGCTGGTCGAGGACGACAAGCTCGGCAAGGCCATGGAGGCCGCGCTGTCGGCGGTGGACGCGGCCTCGCTT
TCGGGCGACGAAAGTGAATTCGACGACATCGACCTGGAGGCGGGCGGGCGCGAGGAACAGGCGGTGGACGCCTCGGCGAC
CCAAAGCGACGCCGACGATGCGCCTGTGGTGCGCTACGTCAACAAGGTGCTGCTCGACGCGATCCGCCGCGGTGCCTCGG
ACATCCATTTCGAACCCTACGAAAAGTACTACCGCGTGCGCTTGCGCGTCGACGGCGAGTTGCGTGAGTTCGCCCGGCCA
CCGGTGCAGATCGCGGCCAGACTGGCGGCGCGCGTCAAGGTGCTGTCGCGCCTCGACCTGGCCGAGCGGCGCGTGCCGCA
GGACGGGCGCATCAAGCTCAAGCTGTCGGCACAGCGTGCCATTGATTTCCGTGTCAGCACCTGCCCCACGCTCTGGGGCG
AGAAGATCGTGCTGCGTATTCTCGATTCCAGCAGCGCACAGCTCGGCATCGAATCGCTGGGCTACGAGCCCGAGCAGCGG
GCACTCTATGAGAATGCGCTGTCCAAGCCGCAGGGCATGATTCTGGTGACCGGGCCGACCGGCTCCGGCAAGACCGTCTC
GCTGTACACCGGTTTGAATATCCTCAATACCGAGGGGCTGAATATCTCCACCGCCGAGGACCCGGCGGAAATCAACCTTC
CCGGCATCAATCAGGTGAATGTCAATCCGCGTGTGGGGCTGACCTTTGCCGCGGCATTGAAGGCCTTTCTCCGCCAGGAT
CCGGACGTGATCATGGTGGGTGAGGTTCGGGACCTGGAAACGGCCGAGATCGCAATCAAGGCGGCACAGACCGGGCACCT
TGTGTTGTCCACACTGCATACCAACGATGCGCCGCAGACGCTGACCCGCCTGCTCAATATGGGGGTACCGGCCTACAACA
TCGCCTCCAGCGTGTCGCTGATCATCGCGCAGCGGCTGGCGAAACGGCTGTGCGTGCATTGCCGCAAGGAAGCGGATATT
CCGCGCGTGGAGCTGCTCAAGGAGGGTTTCACCGAGGAACAGGTAGACAGCGAAGGGTTCACGATCTACGAAGCCCAGGG
CTGCGACGAATGCCACAGCGGCTACAAGGGGCGTACCGGTATCTATCAGGTGATGCCGGTCTCCGAGACCATCGGTCGCA
TGATCATGGAAGGCAAGAACGCTATCGATGTCGCCGACCAGGCCGAGCGCGAGGGCGTTCTGGATCTGCGCCAGTCGGCC
TTGAAGAAAGTGCGGGACGGCATTATTGACCTGAAAGAAGCCAACCGCGTGACGGTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.27

97.241

0.557
  pilB Acinetobacter baylyi ADP1

54.947

97.586

0.536
  pilB Legionella pneumophila strain ERS1305867

52.281

98.276

0.514
  pilB Vibrio cholerae strain A1552

52.072

95.69

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.241

0.486
  pilF Neisseria gonorrhoeae MS11

49.11

96.897

0.476
  pilB Vibrio campbellii strain DS40M4

48.833

96.034

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

36.232

100

0.388
  pilF Thermus thermophilus HB27

37.522

97.414

0.366

Multiple sequence alignment