Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOFKY_RS14740 Genome accession   NZ_OZ241355
Coordinates   3186860..3188569 (+) Length   569 a.a.
NCBI ID   WP_419819691.1    Uniprot ID   -
Organism   MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3181860..3193569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKY_RS14700 nusA 3182604..3184088 (-) 1485 WP_336952303.1 transcription termination factor NusA -
  ACOFKY_RS14705 rimP 3184123..3184647 (-) 525 WP_419359009.1 ribosome maturation factor RimP -
  ACOFKY_RS14730 secG 3185438..3185779 (-) 342 WP_419819689.1 preprotein translocase subunit SecG -
  ACOFKY_RS14735 tpiA 3185789..3186583 (-) 795 WP_419819690.1 triose-phosphate isomerase -
  ACOFKY_RS14740 pilB 3186860..3188569 (+) 1710 WP_419819691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOFKY_RS14745 - 3188601..3189833 (+) 1233 Protein_2897 type II secretion system F family protein -
  ACOFKY_RS14750 pilD 3189836..3190717 (+) 882 WP_419819692.1 prepilin peptidase Machinery gene
  ACOFKY_RS14755 coaE 3190744..3191343 (+) 600 WP_419819693.1 dephospho-CoA kinase -
  ACOFKY_RS14760 - 3191738..3192658 (-) 921 WP_336952313.1 DMT family transporter -
  ACOFKY_RS14765 rlmB 3192697..3193452 (-) 756 WP_336952314.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63280.87 Da        Isoelectric Point: 5.0202

>NTDB_id=1170782 ACOFKY_RS14740 WP_419819691.1 3186860..3188569(+) (pilB) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDAFSNAQKNKVSMVAYLSEKLNIPPLIIAEMISVEFGEPIFDLDSFNPEML
LKEIVDPSIVTKHKVIPLVQRGNILYVATSDPTNIEALDAIRFNCGLNIEPIIVEHHKLEKLLEKHFTVEESFNFGDEDF
DVDVIATDDQPPSEDDDASEEEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLASRLASR
LKVMSRMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEKQKALFMEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPVEIPRQSLLEMGFT
EQDLSNPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLIKVINGLTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1170782 ACOFKY_RS14740 WP_419819691.1 3186860..3188569(+) (pilB) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGATATATCCGTCGCTTAGTCGACGAAGGTTTTTTATCTGCCCAGCA
AATGAAAGATGCGTTCAGTAATGCTCAAAAAAATAAAGTAAGTATGGTTGCTTATCTCAGTGAAAAATTAAATATACCAC
CACTGATCATTGCCGAAATGATCTCCGTTGAGTTTGGTGAGCCTATTTTTGATCTGGACAGCTTTAATCCTGAAATGCTA
CTCAAGGAGATTGTTGATCCGAGCATAGTTACTAAGCATAAAGTGATCCCTTTGGTGCAACGCGGTAATATTTTATATGT
TGCCACCAGTGATCCGACCAATATAGAAGCGCTGGATGCCATTCGTTTTAACTGTGGTCTGAATATTGAACCGATTATTG
TTGAACACCATAAATTAGAAAAACTATTAGAAAAACACTTTACGGTAGAAGAAAGTTTTAACTTTGGTGATGAAGATTTT
GATGTTGATGTCATTGCTACCGATGACCAACCTCCATCTGAAGATGATGATGCATCTGAAGAAGAAGCGCCTATTGTTAA
ATATATCAATAAACTCTTGGTCGATGCGATTCGCATGGGGGCATCAGATTTACACTTTGAGCCTTATGAGAAAAGTTATC
GAGTGCGTTATCGAGTAGATGGTGTGCTTAGAACCATTACCAATCCGCCCTTACAACTGGCAAGTCGTTTGGCATCACGC
CTAAAAGTCATGTCCAGAATGGATATTTCAGAAAAGCGTGTCCCGCAAGATGGTCGGATCAAATTAAAACTCTCAAAAAC
CAAAGCGATTGATTTTCGTGTGAATTCATTACCTACCTTGTTTGGTGAAAAATTGGTGCTGCGTATTCTTGATCCATCGA
GTGCCATGCTGGGTATTGATGCGCTCGGATATGAAGAAAAGCAAAAAGCTTTATTTATGGAGGCTTTAGAGAAACCTCAA
GGCATGTTACTCATCACGGGGCCAACAGGTTCTGGTAAAACTGTTTCCCTTTATACTGGCTTAAATATCCTGAATACAGA
AAGTTCAAATATTTCTACAGCTGAAGATCCAGTTGAAATTAACTTGGAAGGGATTAATCAAGTCAATGTTAACCTCAAGG
CAGGTCTCACCTTTTCAGTCGCATTAAAATCATTCCTACGTCAAGACCCTGACATTATCATGGTCGGAGAGATTCGTGAC
CTTGAAACTGCTGAAATTGCAATTAAGGCTGCTCAAACGGGTCATATGGTGATGTCTACGCTACATACCAACAGTGCGCC
AGAAACGTTGACCCGTTTACGCAATATGGGAGTCCCCTCATTTAACATTGCCACTTCGGTGAATCTTGTGATTGCACAAC
GATTGGCAAGACGCTTATGTAATCATTGTAAAGCACCTGTTGAAATTCCTAGACAGAGTTTGTTAGAAATGGGCTTTACT
GAACAAGACTTAAGTAATCCTGAGTTACAAATTTTTCAACCTGTAGGCTGTAGTGAATGCCGTGAAGGCTATAAAGGGCG
TGTCGGGATCTATGAAGTCATGAAAGTAACACCTGAGATTTCAAAAATTATCATGGAAGATGGCAATGCACTTCAAATTG
CAGCGGCATCGGAGAAATTGGGGTTTGATAATCTAAGACGTTCAGGCTTAATTAAGGTCATCAATGGCTTAACTTCTTTA
CAAGAAATTAACCGTGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.322

100

0.787
  pilB Acinetobacter baylyi ADP1

75.088

100

0.752
  pilB Legionella pneumophila strain ERS1305867

50.523

100

0.51
  pilF Neisseria gonorrhoeae MS11

49.198

98.594

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.571

98.418

0.478
  pilB Vibrio campbellii strain DS40M4

47.576

97.891

0.466
  pilB Vibrio cholerae strain A1552

50.485

90.51

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

34.095

100

0.367

Multiple sequence alignment