Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACMTA2_RS03295 Genome accession   NZ_OZ221631
Coordinates   747417..749504 (+) Length   695 a.a.
NCBI ID   WP_413793697.1    Uniprot ID   -
Organism   Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742417..754504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMTA2_RS03275 pilM 744626..745690 (+) 1065 WP_413793693.1 pilus assembly protein PilM Machinery gene
  ACMTA2_RS03280 - 745690..746256 (+) 567 WP_413793694.1 PilN domain-containing protein -
  ACMTA2_RS03285 pilO 746253..746876 (+) 624 WP_413793695.1 type 4a pilus biogenesis protein PilO -
  ACMTA2_RS03290 - 746873..747403 (+) 531 WP_413793696.1 pilus assembly protein PilP -
  ACMTA2_RS03295 pilQ 747417..749504 (+) 2088 WP_413793697.1 type IV pilus secretin PilQ Machinery gene
  ACMTA2_RS03300 aroK 749509..750027 (+) 519 WP_024616288.1 shikimate kinase AroK -
  ACMTA2_RS03305 aroB 750176..751276 (+) 1101 WP_413793698.1 3-dehydroquinate synthase -
  ACMTA2_RS03310 - 751287..752861 (+) 1575 WP_413793699.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75091.27 Da        Isoelectric Point: 5.5632

>NTDB_id=1170542 ACMTA2_RS03295 WP_413793697.1 747417..749504(+) (pilQ) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MNRIFPALGISLWIALLSPMVHGASLKTLAVAALPGDRVELKLTFDEPPPTPRGYTTEQPARIALDLPGVTNQLASRRHD
LGGGNARSATLVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGMTSNSPASKSTAQAPRPAVASAAPARAGAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGTSTTIGTPGSTGTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=1170542 ACMTA2_RS03295 WP_413793697.1 747417..749504(+) (pilQ) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
ATGAACAGGATTTTTCCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTATCGCCGATGGTTCATGGGGCCAGCCTGAA
GACGCTGGCGGTGGCGGCGTTGCCGGGGGATCGTGTCGAGCTGAAGCTCACCTTCGACGAGCCACCACCGACACCCCGGG
GCTATACCACCGAACAACCGGCCCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTGGCCAGCAGGCGCCACGAC
CTGGGCGGGGGCAATGCACGCAGTGCCACCCTGGTCGAGGCAGGGGATCGCACCCGCCTGATCATCGGCCTGACCCAACT
GGCGCCGTATGACACCCGGATCGAAGGCAACAACCTGTTCGTGGTGGTTGGCAAGGGCATGACCTCCAATAGCCCTGCTT
CGAAGAGCACGGCCCAGGCCCCGCGCCCGGCGGTGGCCAGCGCTGCGCCCGCGAGGGCCGGCGCGCCGGTGGGCAAGGCC
ATTCGTGGGGTGGATTTCCAGCGGGGGACCCAGGGCGAAGGCAACGTGGTGATCGATTTGTCCGATCCGTCCATCGCCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCGGAGCCACTGCGGGTGCGTCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGTGCCGGCAGCGATCGGGCGACCATCAGCATCGAGCCC
AGCGGCACTTTCGATTATTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGCAATGCCGACCGCGCCGCCTACAGCGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAAGTGCGATCGG
TCCTGCAGTTGATTGCCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGGTTG
CAGAACGTGCCTTGGGACCAGGCACTGGACCTGGTACTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAACGTGCT
GTTGGTGGCTCCGGCCGATGAAATTGCCGCCCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGGCGC
CGCTGCGGCGTGAGTTGTTGCAGGTCAACTATGCGAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCC
GAGGCGAAAGCCGATGAGCGCGGCACGATCACGGTTGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
CCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CTAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGTGGGTCGATACAGAACAAGGGCAACTGGAACTCCTCGGGG
GTGACCACCGGCACCTCCACGACCATCGGCACGCCGGGCAGCACCGGCACCAATCAACCGTTCGTCGACCTGGGCGCCTC
GGGCAACACCTCGGGGATCGGCATTGCGTTCATCACCGACAATGTGTTGCTGGACCTCGAACTGACGGCCATGGAGAAAA
CCGGTAACGGGGAAATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAA
ATTCCTTATCAGGAAGCCAGCTCCAGCGGTGCTACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTGACCCC
GCAGATCACCCCGGACAACCGTATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGTGTT
TTCTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTCTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.538

100

0.753
  pilQ Acinetobacter baumannii D1279779

38.503

100

0.414
  pilQ Acinetobacter baumannii strain A118

38.503

100

0.414
  comQ Acinetobacter baylyi ADP1

41.439

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.824

97.842

0.38
  pilQ Legionella pneumophila strain ERS1305867

38.7

97.41

0.377

Multiple sequence alignment