Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACMTA2_RS03275 Genome accession   NZ_OZ221631
Coordinates   744626..745690 (+) Length   354 a.a.
NCBI ID   WP_413793693.1    Uniprot ID   -
Organism   Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739626..750690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMTA2_RS03265 - 740406..741674 (+) 1269 WP_063323308.1 malic enzyme-like NAD(P)-binding protein -
  ACMTA2_RS03270 - 741973..744417 (-) 2445 WP_413795584.1 penicillin-binding protein 1A -
  ACMTA2_RS03275 pilM 744626..745690 (+) 1065 WP_413793693.1 pilus assembly protein PilM Machinery gene
  ACMTA2_RS03280 - 745690..746256 (+) 567 WP_413793694.1 PilN domain-containing protein -
  ACMTA2_RS03285 pilO 746253..746876 (+) 624 WP_413793695.1 type 4a pilus biogenesis protein PilO -
  ACMTA2_RS03290 - 746873..747403 (+) 531 WP_413793696.1 pilus assembly protein PilP -
  ACMTA2_RS03295 pilQ 747417..749504 (+) 2088 WP_413793697.1 type IV pilus secretin PilQ Machinery gene
  ACMTA2_RS03300 aroK 749509..750027 (+) 519 WP_024616288.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38038.60 Da        Isoelectric Point: 4.9070

>NTDB_id=1170541 ACMTA2_RS03275 WP_413793693.1 744626..745690(+) (pilM) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVIEKNIAELDGVGQALSRVLAKARTPVRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHSGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLGEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1170541 ACMTA2_RS03275 WP_413793693.1 744626..745690(+) (pilM) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
GTGCTACGACTCTTCAATAAAAAAGCCCACGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTCGAGTT
GAGTCGCCAGGGCGAGCGCTACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAATGCCGTGATCGAGAAGAATA
TCGCCGAGCTCGACGGGGTGGGCCAGGCGTTGTCGCGAGTGTTGGCCAAGGCCAGGACCCCCGTGCGCAGCGTCGCCGTG
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGACGACATGGAGAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTTGAAGTGGTGGGCGTTTCGCCGC
GCAGCGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTTCGCGAGGCGGCGTTGGCACTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGCGCGTTTGGATTGCTCGCCACGCAACTGGC
CGCGTCCCAGGAACGGTTGACCGTGGCGGTCATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAGCGGAC
GCATCATCTATACCCGTGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATTCAGCGCCGCTATGGCCTGACGCCC
GAGCAGGCCGGGCTGGCAAAGAAGCAGGGGGGGCTGCCGGACGATTATCTCGGCGAAGTGCTGCAACCTTTTCGCGAAGC
CCTGGTGCAGCAAGTCTCGCGGTCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGCGCGGTGGACCACATCCTGCTGG
CTGGAGGCACGGCTTCGGTGGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTCACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCAGTGACGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.085

100

0.551
  comM Acinetobacter nosocomialis M2

55.085

100

0.551
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment