Detailed information NTG1170376

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: ACLV7D_RS06365
• Genome accession: NZ_OZ217345
• Coordinates: 1300332..1300982 (-)
• Length: 216 a.a.
• NCBI ID: WP_218764116.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis F22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1295332..1305982

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7D_RS06345 (SMIF22_12450)	-	1295886..1297067 (-)	1182	WP_218764120.1	hypothetical protein	-
ACLV7D_RS06350 (SMIF22_12460)	-	1297071..1297658 (-)	588	WP_218764119.1	ATP-binding cassette domain-containing protein	-
ACLV7D_RS06355 (SMIF22_12470)	-	1297691..1297879 (-)	189	WP_411865970.1	hypothetical protein	-
ACLV7D_RS06360 (SMIF22_12480)	comEC/celB	1298108..1300348 (-)	2241	WP_218764117.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLV7D_RS06365 (SMIF22_12490)	comEA/celA/cilE	1300332..1300982 (-)	651	WP_218764116.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLV7D_RS06370 (SMIF22_12500)	-	1301050..1301619 (-)	570	WP_218764115.1	GNAT family N-acetyltransferase	-
ACLV7D_RS06375 (SMIF22_12510)	ald	1301791..1302903 (+)	1113	WP_112443604.1	alanine dehydrogenase	-
ACLV7D_RS06380 (SMIF22_12520)	-	1302960..1303955 (-)	996	WP_218764114.1	PhoH family protein	-
ACLV7D_RS06385 (SMIF22_12530)	-	1304040..1304255 (-)	216	WP_001232085.1	YozE family protein	-
ACLV7D_RS06390 (SMIF22_12540)	cvfB	1304264..1305118 (-)	855	WP_218764113.1	RNA-binding virulence regulatory protein CvfB	-
ACLV7D_RS06395 (SMIF22_12550)	frr	1305178..1305735 (-)	558	WP_001262225.1	ribosome recycling factor	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23146.34 Da
• Isoelectric Point: 6.0661

>NTDB_id=1170376 ACLV7D_RS06365 WP_218764116.1 1300332..1300982(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis F22]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQAEVAAVSKDSSTEKEVNKEEKEEALEQDLITVDVKG
AVKSPGIYDLPVGSRVNNAVQKAGGLTDQADSKSLNLAQKVSDEALVYVPTKGEETASQQTASGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1170376 ACLV7D_RS06365 WP_218764116.1 1300332..1300982(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis F22]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTACTGGTCTGGGCTTGCTTGTTGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACATCTGTAAAAGAAACGAATTTACAGGCAGAAGTTGCAGCTGTTTCCAAGGATT
CATCGACCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAAGCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCTCCGGGTATTTACGATTTACCGGTAGGTAGTAGAGTCAATAATGCCGTGCAGAAAGCAGGTGGCTTGAC
AGATCAAGCAGATAGCAAGTCGCTCAATCTAGCTCAGAAAGTCAGTGATGAGGCTCTGGTTTACGTTCCAACTAAGGGGG
AAGAAACAGCTAGTCAACAGACTGCTTCTGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGATATTATCGACCATCGTGAGGCAAATGG
GAAATTTAAGTCGGTTGATGAACTCAAGAAAGTTTCTGGTATTGGTGCCAAGACCATAGAAAAGCTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis NCTC 12261	94.444	100	0.944
comEA/celA/cilE	Streptococcus pneumoniae Rx1	93.981	100	0.94
comEA/celA/cilE	Streptococcus pneumoniae D39	93.981	100	0.94
comEA/celA/cilE	Streptococcus pneumoniae R6	93.981	100	0.94
comEA/celA/cilE	Streptococcus mitis SK321	91.204	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	35.193	100	0.38