Detailed information NTG1170293

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: ACLV7A_RS05850
• Genome accession: NZ_OZ217344
• Coordinates: 1196075..1196725 (-)
• Length: 216 a.a.
• NCBI ID: WP_411863847.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis E22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1191075..1201725

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7A_RS05830 (SMIE22_11520)	-	1191690..1192874 (-)	1185	WP_411863844.1	hypothetical protein	-
ACLV7A_RS05835 (SMIE22_11530)	-	1192878..1193465 (-)	588	WP_411863845.1	ATP-binding cassette domain-containing protein	-
ACLV7A_RS05840	-	1193498..1193693 (-)	196	Protein_1108	hypothetical protein	-
ACLV7A_RS05845 (SMIE22_11540)	comEC/celB	1193851..1196091 (-)	2241	WP_411863846.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLV7A_RS05850 (SMIE22_11550)	comEA/celA/cilE	1196075..1196725 (-)	651	WP_411863847.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLV7A_RS05855 (SMIE22_11560)	-	1196792..1197361 (-)	570	WP_411863848.1	GNAT family N-acetyltransferase	-
ACLV7A_RS05860 (SMIE22_11570)	-	1197468..1198472 (-)	1005	WP_411863849.1	PhoH family protein	-
ACLV7A_RS05865 (SMIE22_11580)	-	1198557..1198772 (-)	216	WP_095726103.1	YozE family protein	-
ACLV7A_RS05870 (SMIE22_11590)	cvfB	1198781..1199635 (-)	855	WP_001095456.1	RNA-binding virulence regulatory protein CvfB	-
ACLV7A_RS05875 (SMIE22_11600)	frr	1199695..1200252 (-)	558	WP_001262225.1	ribosome recycling factor	-
ACLV7A_RS05880 (SMIE22_11610)	pyrH	1200261..1200998 (-)	738	WP_000002996.1	UMP kinase	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23298.54 Da
• Isoelectric Point: 5.5209

>NTDB_id=1170293 ACLV7A_RS05850 WP_411863847.1 1196075..1196725(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis E22]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPDSQTPVKETNLQAEVAAVSKDSVSEKEVKKEEKEEPFEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAASQHTSSGTASSTSKDKKINLNKA
SLEELKQVKGLGGKRAQDIIDYREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1170293 ACLV7A_RS05850 WP_411863847.1 1196075..1196725(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis E22]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGATTCACAAACACCTGTCAAGGAAACGAATTTGCAGGCAGAAGTTGCAGCTGTTTCCAAGGATT
CGGTATCCGAAAAGGAAGTGAAGAAGGAAGAAAAGGAAGAACCCTTTGAACAAGACCTAATCACAGTAGATGTTAAGGGG
GCTGTTAAATCGCCAGGGATTTATGATTTGCCGGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGATAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCCACTAAGGGAG
AAGAAGCAGCTAGTCAACATACTAGTTCGGGGACAGCTTCTTCAACAAGCAAGGACAAGAAGATCAATCTCAACAAGGCC
AGTCTGGAAGAGCTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGATATTATTGACTATCGTGAGGCAAATGG
GAAATTTAAGTCGGTTGATGAACTCAAGAAGGTCTCTGGCATTGGCGCCAAGACTATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (152-214)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	94.907	100	0.949
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	94.444	100	0.944
comEA/celA/cilE	Streptococcus pneumoniae D39	93.519	100	0.935
comEA/celA/cilE	Streptococcus pneumoniae R6	93.519	100	0.935
comEA/celA/cilE	Streptococcus pneumoniae Rx1	93.519	100	0.935
comEA/celA/cilE	Streptococcus mitis SK321	92.593	100	0.926
comEA	Lactococcus lactis subsp. cremoris KW2	42.342	100	0.435
comEA	Latilactobacillus sakei subsp. sakei 23K	34.615	100	0.375