Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACFCTL_RS05150 Genome accession   NZ_OZ182546
Coordinates   1107865..1108929 (+) Length   354 a.a.
NCBI ID   WP_013101265.1    Uniprot ID   A0A378K321
Organism   Legionella pneumophila isolate ZIB8567     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1102865..1113929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCTL_RS05140 - 1103907..1106291 (-) 2385 WP_027226227.1 penicillin-binding protein 1A -
  ACFCTL_RS05145 - 1106441..1107448 (-) 1008 WP_013101264.1 hypothetical protein -
  ACFCTL_RS05150 pilM 1107865..1108929 (+) 1065 WP_013101265.1 type IV pilus assembly protein PilM Machinery gene
  ACFCTL_RS05155 pilN 1108933..1109481 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  ACFCTL_RS05160 pilO 1109495..1110094 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFCTL_RS05165 pilP 1110091..1110675 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  ACFCTL_RS05170 pilQ 1110679..1112775 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  ACFCTL_RS05175 aroK 1113222..1113749 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38829.84 Da        Isoelectric Point: 5.0367

>NTDB_id=1167974 ACFCTL_RS05150 WP_013101265.1 1107865..1108929(+) (pilM) [Legionella pneumophila isolate ZIB8567]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPTSALEGNMIKDVDVVANCIKKLVERLRFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1167974 ACFCTL_RS05150 WP_013101265.1 1107865..1108929(+) (pilM) [Legionella pneumophila isolate ZIB8567]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTTTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGGGAAGAAATACCAACCTCTGCTCTTGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAGGTTTACATCTAAAAATGTGGCATTG
GCTGTGCCTGATTCAGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGATTGACTGATCTTGAAATGGAGGAGCTTGT
CGTTATTGAGGCGGATAAGTATATCCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGTTCATCACTGCTTGATGTTTTAATTGTGGCTTCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCTCATAGA
GCAGGGTTGGAACCTAAAATTGTTGATGTTGAATCTTATGCAGTAGAGCGCTCAGTACAACAAATTGCGCAAGAATTACC
AGCTGGGGGACAAGACAAAATTGTGGCAATAGTAGATATTGGAGCCAATTATACTCACTTGTTTGTTTTGCACGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGTGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGTTTTGTAGACCATATTTTATTGG
CAGGAGGAATGGCCAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAACCCG
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAATGATGCGCCTACACTAATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K321

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

98.588

100

0.986
  comM Acinetobacter nosocomialis M2

42.938

100

0.429
  pilM Acinetobacter baumannii D1279779

42.655

100

0.427
  comM Acinetobacter baylyi ADP1

41.457

100

0.418

Multiple sequence alignment