Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACFCTL_RS05155 Genome accession   NZ_OZ182546
Coordinates   1108933..1109481 (+) Length   182 a.a.
NCBI ID   WP_013101266.1    Uniprot ID   A0A378K2G2
Organism   Legionella pneumophila isolate ZIB8567     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1103933..1114481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFCTL_RS05145 - 1106441..1107448 (-) 1008 WP_013101264.1 hypothetical protein -
  ACFCTL_RS05150 pilM 1107865..1108929 (+) 1065 WP_013101265.1 type IV pilus assembly protein PilM Machinery gene
  ACFCTL_RS05155 pilN 1108933..1109481 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  ACFCTL_RS05160 pilO 1109495..1110094 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACFCTL_RS05165 pilP 1110091..1110675 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  ACFCTL_RS05170 pilQ 1110679..1112775 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  ACFCTL_RS05175 aroK 1113222..1113749 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21336.10 Da        Isoelectric Point: 10.1636

>NTDB_id=1167975 ACFCTL_RS05155 WP_013101266.1 1108933..1109481(+) (pilN) [Legionella pneumophila isolate ZIB8567]
MTEINLLPWREQRREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLINNQMNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDIVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
DVQKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1167975 ACFCTL_RS05155 WP_013101266.1 1108933..1109481(+) (pilN) [Legionella pneumophila isolate ZIB8567]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAGACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGGATTATCATTGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTCTCTCATTTAATAAATAATCAAATGAACCGAA
ACCAGATTTTACAAAAAGAAATTACTGCTTTAGATGAGCAGATCAAGGAGATTAAGGCACTAAAACAAATCAGGGAAGGT
TTGATTTCGAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGGACTTTAATGGTTCACTTATTTGATGAATTAATAAA
AATTACTCCTCCAGGCGTTTACATAACCAAGATGCAAAGAGAACACGATATAGTAACACTCTGGGGGTATTCTGAGTCGA
ATACAAGTATTTCTATTTTAATGAGAAATATAGAGGCTAATGTCTGGATACAGAATCCTGTCCTAACTGAAATTAAAAAA
GATGTTCAAAAAAAACAGTCTCCAAATAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

96.703

100

0.967

Multiple sequence alignment