Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABNT83_RS01890 Genome accession   NZ_OZ026884
Coordinates   395760..397472 (-) Length   570 a.a.
NCBI ID   WP_348758753.1    Uniprot ID   -
Organism   Candidatus Methylocalor cossyra isolate MeCH1-AG     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390760..402472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNT83_RS01865 (MECH1_V1_0386) - 391912..392115 (-) 204 WP_348758748.1 DNA gyrase inhibitor YacG -
  ABNT83_RS01870 (MECH1_V1_0387) zapD 392140..392946 (-) 807 WP_348758749.1 cell division protein ZapD -
  ABNT83_RS01875 (MECH1_V1_0388) coaE 393000..393638 (-) 639 WP_348758750.1 dephospho-CoA kinase -
  ABNT83_RS01880 (MECH1_V1_0389) - 393648..394523 (-) 876 WP_348758751.1 prepilin peptidase -
  ABNT83_RS01885 (MECH1_V1_0390) pilC 394543..395754 (-) 1212 WP_348758752.1 type II secretion system F family protein Machinery gene
  ABNT83_RS01890 (MECH1_V1_0391) pilB 395760..397472 (-) 1713 WP_348758753.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNT83_RS01895 (MECH1_V1_0392) - 397566..398282 (-) 717 WP_348758754.1 retropepsin-like aspartic protease -
  ABNT83_RS01900 (MECH1_V1_0393) glgA 398294..400444 (-) 2151 WP_348758755.1 glycogen synthase GlgA -
  ABNT83_RS01905 (MECH1_V1_0394) - 400912..402285 (-) 1374 WP_348758756.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62349.05 Da        Isoelectric Point: 6.4438

>NTDB_id=1163282 ABNT83_RS01890 WP_348758753.1 395760..397472(-) (pilB) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
MATSAPTPQLGGLAKSLVKERLLSEAEAQSHFEAALRNNVPFVSYLVNNRILSSSAIAETASKEFGLPLFDLSVIAPEVI
PAKVISEKLIRRHHVLPIFKRGNRLFLAVSDPTNLQALDEVKFQSRLMPESILVEEDKLAKTIDLVLEAADSALKDLLDS
GLDELNVTGGEDLAAVEGELSEADDAPIVRFVNKVLQDAIRKGASDIHFEPYEKHFRIRFRHDGVLHEIASPPVALAGRI
AARLKVMARLDIAERRVPQDGRIKLALSRSHSIDFRVNTCPTLFGEKIVLRILDPSAAQIGIERLGFEPEQQELFLKAIS
KPYGMILVTGPTGSGKTVTLYTALNILNQPNVNISTAEDPVEITVPGINQVNVNIKTGLTFAECLRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLVQMGIPPFNIASSVLLIMAQRLARRLCPHCKREEKLPPEVLLQA
GFRSSELDGLTIYGPVGCDECVKGYKGRVGIYQVMPITDAINRLILEGGNAVRISEQAKAEGVSDLRESGLKKVKAGITS
LAEIDRVTRD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1163282 ABNT83_RS01890 WP_348758753.1 395760..397472(-) (pilB) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
ATGGCCACCTCGGCTCCCACGCCGCAGCTCGGCGGGCTGGCGAAAAGCCTTGTCAAGGAACGGCTTTTATCGGAAGCGGA
GGCGCAGTCCCATTTCGAAGCGGCGCTTAGGAACAACGTTCCCTTTGTGAGCTACCTGGTCAACAACCGCATTCTTTCGA
GCAGTGCCATCGCCGAGACCGCCTCGAAGGAATTCGGCCTTCCCCTGTTCGATCTCAGCGTCATCGCGCCGGAAGTGATC
CCAGCCAAGGTGATCAGCGAGAAGCTCATCCGCCGGCACCATGTGCTCCCCATCTTTAAGCGGGGTAACCGGCTGTTTCT
GGCGGTTTCCGATCCCACCAACCTACAGGCCCTTGACGAGGTCAAGTTTCAATCCCGCCTCATGCCGGAGAGCATTCTGG
TGGAGGAGGACAAGCTCGCCAAAACCATCGATCTGGTGCTGGAGGCGGCGGACAGCGCTCTCAAGGACCTGCTCGATTCC
GGCCTCGACGAACTGAACGTGACCGGCGGTGAGGATCTCGCAGCGGTGGAAGGGGAGCTTAGCGAGGCCGACGACGCTCC
CATTGTGCGTTTCGTCAACAAAGTGCTGCAGGACGCCATCCGGAAGGGCGCCTCGGACATACATTTCGAGCCGTACGAGA
AGCATTTCCGGATTCGCTTCCGTCACGACGGGGTTCTCCACGAGATCGCCTCCCCGCCGGTGGCTCTCGCCGGGCGCATC
GCGGCGCGCCTTAAGGTCATGGCGCGCTTGGATATCGCCGAGCGTCGAGTGCCCCAAGACGGCCGCATCAAGCTGGCCCT
GTCCCGGTCCCACTCCATCGACTTCCGGGTGAACACCTGCCCCACGCTGTTCGGGGAAAAAATCGTGCTGCGCATCCTGG
ATCCCAGCGCGGCCCAGATCGGTATCGAACGGCTTGGCTTCGAGCCGGAGCAGCAGGAGCTGTTCCTCAAGGCCATCAGC
AAGCCCTACGGCATGATCCTGGTGACGGGCCCGACCGGCAGCGGCAAGACCGTGACGCTCTACACCGCCCTCAATATCCT
GAACCAGCCGAACGTCAACATTTCCACGGCGGAGGATCCGGTGGAGATCACCGTGCCGGGCATCAATCAGGTGAACGTCA
ACATCAAGACCGGCCTCACCTTCGCCGAGTGCCTGCGGGCCTTCCTGCGGCAGGATCCGGACATCATCATGGTGGGGGAG
ATCCGTGACCTGGAAACCGCTGAAATCGCCGTCAAGGCGGCGCAGACCGGGCACCTAGTGCTGTCCACCTTGCACACCAA
TGATGCGCCGCAAACCCTGAACCGGCTGGTGCAAATGGGTATACCGCCGTTCAACATCGCCTCATCGGTGCTGTTGATCA
TGGCCCAGCGGTTAGCCCGCCGCCTGTGCCCGCACTGCAAGCGGGAGGAAAAGCTACCGCCGGAAGTCCTGTTGCAGGCC
GGGTTCCGTTCGTCCGAACTGGACGGTCTGACCATCTACGGTCCGGTCGGGTGCGACGAATGCGTGAAGGGGTACAAGGG
GCGCGTGGGTATTTATCAGGTCATGCCCATCACTGACGCTATCAACCGTCTCATCCTCGAGGGGGGGAATGCGGTGCGGA
TCAGCGAGCAGGCGAAAGCGGAGGGCGTCAGCGACCTCCGGGAATCAGGGTTGAAAAAAGTCAAAGCGGGTATCACCAGC
TTGGCGGAAATCGATCGTGTCACCCGGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.56
  pilB Acinetobacter baumannii D1279779

55.035

100

0.556
  pilB Acinetobacter baylyi ADP1

54.181

100

0.546
  pilB Vibrio cholerae strain A1552

53.551

91.404

0.489
  pilF Neisseria gonorrhoeae MS11

49.201

98.772

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

48.122

98.07

0.472
  pilB Vibrio campbellii strain DS40M4

47.331

98.596

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

40.653

96.667

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.315

92.456

0.391
  pilF Thermus thermophilus HB27

41.746

92.456

0.386

Multiple sequence alignment