Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Z042_RS22960 Genome accession   NZ_CP007044
Coordinates   5155535..5156968 (+) Length   477 a.a.
NCBI ID   WP_024910384.1    Uniprot ID   W0LG13
Organism   Chania multitudinisentens RB-25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5150535..5161968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Z042_RS22935 (Z042_17680) erpA 5150959..5151303 (-) 345 WP_024910389.1 iron-sulfur cluster insertion protein ErpA -
  Z042_RS22940 (Z042_17675) hemL 5151590..5152879 (+) 1290 WP_024910388.1 glutamate-1-semialdehyde 2,1-aminomutase -
  Z042_RS22945 (Z042_17670) ampE 5152918..5153763 (-) 846 WP_024910387.1 beta-lactamase regulator AmpE -
  Z042_RS22950 (Z042_17665) nadC 5153994..5154884 (+) 891 WP_024910386.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Z042_RS22955 (Z042_17660) ppdD 5155087..5155524 (+) 438 WP_024910385.1 prepilin peptidase-dependent pilin -
  Z042_RS22960 (Z042_17655) pilB 5155535..5156968 (+) 1434 WP_024910384.1 type II secretion system protein GspE Machinery gene
  Z042_RS22965 (Z042_17650) hofC 5156968..5158167 (+) 1200 WP_024910383.1 protein transport protein HofC -
  Z042_RS22970 (Z042_17645) - 5158225..5159268 (-) 1044 WP_024910382.1 GMP reductase -
  Z042_RS22975 (Z042_26450) coaE 5159633..5160238 (+) 606 WP_037405599.1 dephospho-CoA kinase -
  Z042_RS22980 (Z042_17635) zapD 5160253..5161005 (+) 753 WP_024910381.1 cell division protein ZapD -
  Z042_RS22985 (Z042_17630) yacG 5161047..5161241 (+) 195 WP_024910380.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 52571.32 Da        Isoelectric Point: 5.4590

>NTDB_id=116314 Z042_RS22960 WP_024910384.1 5155535..5156968(+) (pilB) [Chania multitudinisentens RB-25]
MEISDEIHALCQRYQALALSESSQQLTVALHGDIPPTLVQALRFAAGKSIVVEQWPLARLEQAQNQWRAPEIALQPEALE
DSAPSLNDDSDTPVVQFINQALRHAIQRRASDVHFEPYQQTLRVRLRIDGVLQAITSPPLSLAARITARLKIMGQLDITE
RRLPQDGQLSVQLDNTSFSMRISTLPTLYGEKVVLRILQTDRQELLLEQLGMNQAELGLYAKALASPQGMILVTGPTGSG
KTVTLYSGLRQLNDVQSNLCSVEDPIEIPVFGINQTQVNSKTELDFARVLRALLRQDPDVIMIGEIRDRETAEIAVKAAQ
TGHLVLSTLHTNSTSETLTRLTQMGIPGYLLAACLKLIIAQRLVRRLCSHCRSPHSETMRFPVNLWPEPLVPWSAAGCEH
CFGGYYGRIGLYELLTITPEVQNALLQNASAVQLADIAQQQGQMTLLQAGLALVAQGITSLEEVYRVVGTTTDSEAI

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=116314 Z042_RS22960 WP_024910384.1 5155535..5156968(+) (pilB) [Chania multitudinisentens RB-25]
ATGGAAATCAGTGATGAAATCCACGCGCTATGCCAGCGCTATCAGGCGCTGGCGCTCAGCGAAAGCTCACAACAGCTGAC
GGTCGCTCTGCACGGCGATATCCCCCCTACTCTGGTTCAAGCACTGCGTTTCGCCGCTGGCAAAAGTATTGTGGTTGAAC
AATGGCCTCTTGCTCGCCTGGAACAGGCACAAAATCAGTGGCGCGCCCCCGAGATCGCCCTACAGCCAGAGGCTCTAGAA
GACAGCGCGCCTTCGCTTAACGATGACAGCGACACCCCAGTAGTACAATTTATCAATCAGGCCCTTCGCCATGCCATTCA
ACGCAGGGCCTCTGACGTGCATTTTGAACCCTACCAACAGACACTCCGCGTCCGCTTAAGGATCGATGGTGTTTTACAGG
CCATTACTTCCCCACCTTTGTCACTGGCAGCACGTATTACTGCCAGGTTGAAAATCATGGGGCAATTGGATATTACCGAA
CGTCGTCTACCCCAAGACGGCCAACTCAGCGTCCAGTTGGACAATACCAGTTTCTCAATGCGTATTTCGACTCTGCCAAC
GTTGTACGGGGAAAAGGTGGTACTGAGGATCTTGCAAACCGATCGCCAAGAACTGTTGCTCGAACAGCTTGGAATGAACC
AGGCAGAACTTGGGCTTTATGCCAAAGCTCTTGCCAGCCCACAGGGGATGATTCTGGTCACCGGCCCCACCGGCAGTGGC
AAAACCGTTACGCTCTACAGCGGCCTACGCCAGTTGAATGATGTGCAGAGCAATCTGTGCAGCGTGGAAGATCCCATCGA
AATCCCGGTGTTTGGCATTAATCAGACCCAGGTCAACAGCAAAACCGAACTGGATTTTGCCCGTGTGTTACGGGCTCTGC
TGCGCCAAGACCCTGATGTCATCATGATTGGTGAGATCCGTGATAGAGAAACTGCCGAGATCGCAGTAAAAGCCGCACAA
ACCGGGCACCTGGTGCTTTCTACTCTGCACACCAACTCCACCAGTGAAACCCTGACGCGCCTGACCCAAATGGGCATTCC
CGGTTACCTGCTGGCGGCTTGCCTGAAGCTGATCATCGCGCAACGGTTAGTACGGCGTTTATGTTCACACTGCCGTTCTC
CACACAGTGAAACCATGCGTTTCCCGGTAAATCTCTGGCCTGAACCGTTGGTTCCCTGGAGTGCCGCTGGTTGTGAACAT
TGCTTTGGCGGCTATTACGGCCGTATTGGCCTCTACGAACTGTTGACGATCACCCCTGAGGTACAGAACGCACTGCTACA
AAATGCCTCAGCGGTTCAATTGGCTGACATTGCACAGCAGCAGGGGCAAATGACACTACTGCAAGCCGGTTTAGCTCTGG
TGGCACAAGGCATTACCTCCCTCGAAGAAGTGTACCGCGTGGTAGGTACAACAACAGACAGCGAGGCAATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(100-366)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0LG13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

47.531

100

0.484
  pilB Vibrio campbellii strain DS40M4

43.952

100

0.457
  pilB Acinetobacter baylyi ADP1

43.737

100

0.447
  pilB Acinetobacter baumannii D1279779

44.167

100

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

46.667

91.195

0.426
  pilB Legionella pneumophila strain ERS1305867

49.492

82.6

0.409
  pilB Haemophilus influenzae Rd KW20

40.842

99.581

0.407
  pilB Haemophilus influenzae 86-028NP

40.169

99.161

0.398
  pilB Glaesserella parasuis strain SC1401

40

98.532

0.394
  pilF Neisseria gonorrhoeae MS11

47.273

80.713

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

41.388

87.631

0.363

Multiple sequence alignment