Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACKPCU_RS00335 Genome accession   NZ_OY781932
Coordinates   77137..79206 (-) Length   689 a.a.
NCBI ID   WP_321349095.1    Uniprot ID   -
Organism   MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 72137..84206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCU_RS00320 (U2971_RS00320) - 73908..75455 (-) 1548 WP_321349092.1 AAA family ATPase -
  ACKPCU_RS00325 (U2971_RS00325) aroB 75452..76531 (-) 1080 WP_321349093.1 3-dehydroquinate synthase -
  ACKPCU_RS00330 (U2971_RS00330) aroK 76622..77131 (-) 510 WP_321349094.1 shikimate kinase AroK -
  ACKPCU_RS00335 (U2971_RS00335) pilQ 77137..79206 (-) 2070 WP_321349095.1 type IV pilus secretin PilQ Machinery gene
  ACKPCU_RS00340 (U2971_RS00340) pilP 79263..79775 (-) 513 WP_321351903.1 type 4a pilus biogenesis lipoprotein PilP -
  ACKPCU_RS00345 (U2971_RS00345) - 79790..80413 (-) 624 WP_321349096.1 type 4a pilus biogenesis protein PilO -
  ACKPCU_RS00350 (U2971_RS00350) - 80410..80988 (-) 579 WP_321349097.1 PilN domain-containing protein -
  ACKPCU_RS00355 (U2971_RS00355) pilM 80988..82052 (-) 1065 WP_104738104.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 689 a.a.        Molecular weight: 74028.67 Da        Isoelectric Point: 4.4408

>NTDB_id=1161836 ACKPCU_RS00335 WP_321349095.1 77137..79206(-) (pilQ) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MSLTRIMPLAALITLLSPLALAADLQSMDVASLPGDRVELKLSFDEPVTGSPKGYTIDQPARIALDLPGVKNKLGERGRE
LGLGNARSVAVVEAGDRTRLIINLTSLAPYTTRAEGNDLYVVVGASAAAQSSAASSPVVTNSNWRTDSEPAVSSPATVSG
RAISSLDFQRGSDGSGNVIIDLTDPTIKVDVEEQGGRVRLNFPNTRLPDELRVQLDVKDFATPVNFVNASGSGNNATIVI
EPTGYYEHLVYQADNRLTVSFKPLTRAEVEQRRADAFTYTGEKLSLNFQDIEVRSVLQLIADFTDLNLVASDTVQGSITL
RLQNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFASVT
AADADSQRGSLTVDERTNSIIALETQQKLDELRRIVTQLDIPVRQVMIEARIVEANVGFDKALGVNWSGELSLGNSDKYR
LYGDDGLASTANSPFVDLGVSGATSGIGIGFVTDNTVLDLQLSAMESTGNGEVISQPKVVTSDKETAKILKGAEIPYQEA
SSSGATSTSFADAVLSLEVTPQITPDNQVIMEVIVTKDEPDFSQVVNGVPIIRKNEVNANILVADGETIVIGGVFQSETS
TSQEKVPFLGDLPLLGKMFRRDISAESKTELLIFLTPRIINNGAITLAR

Nucleotide


Download         Length: 2070 bp        

>NTDB_id=1161836 ACKPCU_RS00335 WP_321349095.1 77137..79206(-) (pilQ) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGTCTCTGACCAGAATTATGCCGCTCGCAGCATTGATAACACTGTTGAGTCCGCTGGCCCTTGCGGCCGACCTGCAAAG
CATGGATGTCGCGTCCCTGCCGGGCGATAGGGTTGAGCTGAAATTGAGTTTTGATGAACCGGTTACCGGTTCGCCCAAGG
GCTATACCATTGATCAACCTGCCCGGATTGCACTTGACCTGCCCGGGGTCAAGAACAAGCTCGGTGAGCGCGGTCGCGAG
TTGGGTCTTGGTAATGCCCGGAGCGTTGCGGTTGTCGAAGCGGGCGATCGAACTCGTCTGATTATCAACCTGACCAGTCT
CGCGCCTTACACCACACGTGCTGAAGGTAATGACCTCTACGTAGTGGTGGGTGCTTCCGCAGCAGCTCAGTCATCGGCGG
CTAGCTCTCCGGTCGTCACCAACAGCAATTGGCGAACCGACAGCGAGCCGGCAGTGTCCAGCCCTGCTACGGTTAGCGGC
CGGGCTATCAGTAGTCTGGATTTTCAACGTGGCAGCGACGGTTCTGGCAACGTCATTATTGATCTGACTGATCCGACCAT
CAAGGTGGATGTGGAAGAGCAGGGGGGGCGCGTTAGGCTCAACTTCCCGAACACCCGTCTGCCTGATGAGCTGCGCGTTC
AGCTTGATGTCAAGGATTTCGCGACACCGGTCAACTTTGTCAATGCCAGCGGCAGTGGCAACAATGCGACCATCGTGATT
GAACCGACCGGCTACTATGAGCATCTTGTCTATCAGGCAGATAACCGCCTGACGGTTAGTTTCAAACCGCTGACCCGCGC
CGAGGTCGAGCAGCGTCGTGCTGATGCCTTCACCTACACCGGCGAAAAGTTGTCCCTTAACTTCCAGGATATTGAAGTGC
GCTCGGTATTGCAGTTGATCGCCGACTTCACGGACCTCAACCTGGTCGCCAGTGACACGGTTCAGGGCAGTATCACCCTG
CGTTTGCAGAACGTGCCCTGGGATCAGGCGCTCGATCTGGTCTTGAAGACCAAAGGATTGGACAAGCGTCAGGTAGGCAA
CGTGCTGCTGGTGGCGCCTGCCGACGAGATTGCTGCACGCGAGCGTCAGGAGCTGGAGGCACAGAAGCAGATTGCCGAGT
TGGCTCCGTTACGCCGTGAGCTGATTCAGGTCAATTACGCCAAGGCGGCAGATATTGCCAAGCTGTTCGCCTCTGTCACG
GCGGCTGATGCGGACTCGCAACGTGGTTCGCTCACGGTTGATGAGCGCACCAACAGCATTATTGCACTTGAGACTCAGCA
GAAGCTCGATGAGCTGCGTCGCATCGTGACCCAGCTCGATATCCCGGTTCGTCAGGTCATGATCGAGGCGCGGATTGTTG
AGGCCAACGTTGGCTTTGACAAGGCACTGGGGGTGAACTGGAGCGGCGAGCTGAGCCTTGGCAACAGTGACAAGTACCGG
CTGTACGGTGACGACGGCCTTGCTTCAACCGCTAATTCCCCGTTTGTTGATCTTGGTGTAAGTGGTGCTACGTCTGGTAT
CGGTATTGGCTTCGTAACGGACAATACTGTTCTCGACTTGCAACTATCCGCCATGGAAAGCACTGGTAATGGTGAGGTTA
TATCCCAGCCGAAGGTGGTAACTTCGGACAAAGAAACCGCTAAGATCCTCAAGGGTGCAGAGATTCCTTATCAGGAAGCA
AGCTCCAGCGGCGCAACCAGTACGTCGTTTGCCGACGCTGTGCTCTCGCTGGAGGTGACTCCGCAGATTACGCCTGACAA
TCAGGTGATCATGGAGGTCATCGTTACCAAGGATGAGCCAGACTTTTCCCAGGTGGTCAACGGTGTGCCGATCATTCGCA
AAAATGAGGTCAATGCGAATATTCTGGTCGCTGATGGCGAAACGATAGTGATTGGCGGCGTATTCCAGAGTGAAACCTCG
ACTTCTCAGGAAAAGGTACCTTTCCTTGGTGACCTGCCCCTGCTGGGCAAGATGTTCCGCCGGGATATCAGTGCCGAAAG
CAAAACTGAGCTGTTGATCTTCCTGACGCCGCGTATTATCAATAACGGTGCAATCACGCTGGCCAGGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(524-680)

AMIN domain (AMIN)

(42-121)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.555

100

0.702
  comQ Acinetobacter baylyi ADP1

42.795

100

0.431
  pilQ Acinetobacter baumannii strain A118

40.59

100

0.419
  pilQ Acinetobacter baumannii D1279779

40.59

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.552

98.258

0.379
  pilQ Legionella pneumophila strain ERS1305867

38.552

98.258

0.379

Multiple sequence alignment