Detailed information NTG1161837

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACKPCU_RS00355
• Genome accession: NZ_OY781932
• Coordinates: 80988..82052 (-)
• Length: 354 a.a.
• NCBI ID: WP_104738104.1
• Uniprot ID: A0A2P4EW26
• Organism: MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 75988..87052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACKPCU_RS00330 (U2971_RS00330)	aroK	76622..77131 (-)	510	WP_321349094.1	shikimate kinase AroK	-
ACKPCU_RS00335 (U2971_RS00335)	pilQ	77137..79206 (-)	2070	WP_321349095.1	type IV pilus secretin PilQ	Machinery gene
ACKPCU_RS00340 (U2971_RS00340)	pilP	79263..79775 (-)	513	WP_321351903.1	type 4a pilus biogenesis lipoprotein PilP	-
ACKPCU_RS00345 (U2971_RS00345)	-	79790..80413 (-)	624	WP_321349096.1	type 4a pilus biogenesis protein PilO	-
ACKPCU_RS00350 (U2971_RS00350)	-	80410..80988 (-)	579	WP_321349097.1	PilN domain-containing protein	-
ACKPCU_RS00355 (U2971_RS00355)	pilM	80988..82052 (-)	1065	WP_104738104.1	pilus assembly protein PilM	Machinery gene
ACKPCU_RS00360 (U2971_RS00360)	-	82237..84636 (+)	2400	WP_321349098.1	penicillin-binding protein 1A	-
ACKPCU_RS00365 (U2971_RS00365)	-	84591..85541 (-)	951	WP_104738102.1	LysR family transcriptional regulator	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37863.32 Da
• Isoelectric Point: 4.3101

>NTDB_id=1161837 ACKPCU_RS00355 WP_104738104.1 80988..82052(-) (pilM) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MLGLFKKKANTLLGIDISSTTVKLLELSRSGGRYKVEAYAVEPLPAGSVVEKNIVELEGVGQALEKVLARSKSSLRQGAV
AVAGSAVITKVIEMDAGLDPDEMEEQIKIEADQYIPYPLDEVAIDFEVQGVSPRNPAREEVLLAACRRENVDIREAALAL
AGLTAKIVEVEAYSMERACELVIGQLDGGDDGLTIAVIDIGATMMTLSVLSGGRTIYSREQLFGGKQLTDEIQRRYGLSQ
EEAGLAKKQGGLPDDYEVEVLGPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDQLVQQKIGTTTLVANP
FANMTLSNKVDAVALSNDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=1161837 ACKPCU_RS00355 WP_104738104.1 80988..82052(-) (pilM) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
GTGCTAGGGCTCTTCAAGAAAAAAGCGAATACGCTGCTGGGCATCGACATCAGCTCCACCACCGTGAAGCTGCTCGAACT
CAGCCGCTCCGGTGGCCGCTACAAGGTCGAGGCCTATGCGGTCGAACCTCTCCCCGCCGGGTCGGTGGTTGAGAAAAATA
TCGTCGAACTGGAAGGCGTCGGACAGGCGCTGGAAAAGGTTCTCGCGCGATCAAAGTCATCTCTCAGACAGGGCGCCGTT
GCGGTAGCCGGGTCCGCGGTCATCACCAAGGTGATCGAAATGGATGCCGGGCTTGATCCTGATGAGATGGAAGAACAGAT
CAAGATCGAAGCCGACCAGTACATTCCCTACCCGCTCGATGAAGTGGCAATTGACTTCGAGGTTCAGGGTGTCTCTCCCC
GAAATCCTGCCAGAGAAGAAGTGCTGCTAGCGGCCTGTCGTCGTGAGAATGTCGATATTCGCGAAGCGGCTCTGGCGTTG
GCCGGGCTGACCGCCAAGATCGTTGAAGTTGAAGCCTACTCGATGGAAAGGGCGTGTGAGTTGGTAATCGGTCAGTTGGA
TGGCGGCGATGATGGCCTGACCATCGCGGTGATCGATATCGGCGCCACGATGATGACGCTAAGCGTGCTTAGCGGTGGGC
GCACCATTTATAGTCGCGAGCAGCTGTTCGGCGGCAAGCAACTGACAGATGAGATCCAGCGCCGTTACGGCCTGTCCCAG
GAGGAAGCTGGGCTGGCCAAGAAGCAGGGCGGCCTGCCGGACGACTATGAAGTCGAAGTGTTGGGCCCCTTCAAGGATGC
GGTGGTTCAGCAAGTTTCGCGCTCGCTTCAGTTCTTTTTCGCGGGTGGCCAGTACAACGATGTTGATTACATACTGCTGG
CCGGTGGTACCGCTTCGATTCCAGGGCTGGATCAGCTGGTACAGCAGAAAATTGGTACCACCACTCTGGTTGCCAACCCC
TTTGCAAATATGACCCTGTCCAACAAGGTTGACGCGGTTGCCCTGAGCAACGATGCGCCGGCCCTGATGATTGCGTGTGG
TCTGGCAATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2P4EW26

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.932	100	0.559
comM	Acinetobacter nosocomialis M2	55.932	100	0.559
comM	Acinetobacter baylyi ADP1	52.542	100	0.525
pilM	Legionella pneumophila strain ERS1305867	45.48	100	0.455